2. Immunology/Inflammation
  3. COX
  4. Macelignan

Macelignan  (Synonyms: (+)-Anwulignan; Anwuligan)

Cat. No.: HY-N0064 Purity: 99.78%
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Macelignan ((+)-Anwulignan; Anwuligan)?is an orally active lignan isolated from Myristica fragrans. Macelignan possesses many pharmacological activities, including anti-inflammatory, anti-cancer, anti-diabetes, and neuroprotective activities.

For research use only. We do not sell to patients.

CAS No. : 107534-93-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Macelignan:

Macelignan Related Antibodies

Top Publications Citing Use of Products

    Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144.  [Abstract]

    RAW264.7 cells and THP-1-derived macrophages were treated with Macelignan (0 - 80 μM) for 24 h, then CCK8 assay was performed to observed cell viability.

    Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144.  [Abstract]

    Levels of CD206, Arg1, and CD163 were measured by WB treated with Macelignan (Mace) (2.5, 5, 10 μM).

    Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144.  [Abstract]

    The proportion of CD206-positive cells was determined by flow cytometer. RAW264.7 cells and THP-1-derived macrophages were exposed to LPS with or without Macelignan (5 μM, 10 μM) for 24 h.

    Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144.  [Abstract]

    Transwell assays were used to measure the migration and invasion of CRC cells treated with Macelignan (Mace) (5, 10 μM).

    Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144.  [Abstract]

    Tumor weight and volume treated with Macelignan (Mace) (5 mg/kg, p.o.).

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Macelignan ((+)-Anwulignan; Anwuligan)?is an orally active lignan isolated from Myristica fragrans. Macelignan possesses many pharmacological activities, including anti-inflammatory, anti-cancer, anti-diabetes, and neuroprotective activities[1][2][3].

    IC50 & Target[1]

    COX-2

     

    In Vitro

    Macelignan (1-50 μM; 72 hours) does not reduce cell viability alone, however, UVB treatment, even at the lowest dose of 30 mJ/cm2, reduces HaCaT cell viability in a dose-dependent mannner,it reduces approximately 80% of control values at 100 μM in hacat cells[1].
    Macelignan (0.1-1 μM; 24 hours) decreases COX-2 expression in a concentration-dependent manner,and at the highest concentration of macelignan (1 μM) , COX-2 expression is inhibited by almost 50% in hacat cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Macelignan Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Hacat cells
    Concentration: 1 μM; 2.5 μM; 5 μM; 10 μM; 15 μM; 50 μM
    Incubation Time: 72 hours
    Result: Induced cell death by UVB irradiation at 30 mJ/cm2 from 10 μM.

    Western Blot Analysis[1]

    Cell Line: Hacat cells
    Concentration: 0.1 μM; 0.5 μM;1 μM
    Incubation Time: 24 hours
    Result: Reduced UVB-induced COX-2 expression in cells.
    In Vivo

    Macelignan (oral administration; 15 mg/kg; every day for three weeks) exhibits?in vivo anti-diabetic effects. The baseline (day 0) fasting blood glucose levels does not differ between groups; at the end of the experiment, the values of the Macelignan-treated group are significantly lower compared to the diabetic control group in C57BL/KsJ-db/db?mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/KsJ-db/db mice[2]
    Dosage: 15 mg/kg
    Administration: Oral administration; 15 mg/kg; every day for three weeks
    Result: Significantly reduced the blood glucose levels in mice.
    Molecular Weight

    328.40

    Formula

    C20H24O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC1=CC=C(C[C@H](C)[C@H](C)CC2=CC=C(OCO3)C3=C2)C=C1OC
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (304.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0451 mL 15.2253 mL 30.4507 mL
    5 mM 0.6090 mL 3.0451 mL 6.0901 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.85%

    SDS (393 KB)

    COA (246 KB) HNMR (192 KB) LCMS (253 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.0451 mL 15.2253 mL 30.4507 mL 76.1267 mL
    5 mM 0.6090 mL 3.0451 mL 6.0901 mL 15.2253 mL
    10 mM 0.3045 mL 1.5225 mL 3.0451 mL 7.6127 mL
    15 mM 0.2030 mL 1.0150 mL 2.0300 mL 5.0751 mL
    20 mM 0.1523 mL 0.7613 mL 1.5225 mL 3.8063 mL
    25 mM 0.1218 mL 0.6090 mL 1.2180 mL 3.0451 mL
    30 mM 0.1015 mL 0.5075 mL 1.0150 mL 2.5376 mL
    40 mM 0.0761 mL 0.3806 mL 0.7613 mL 1.9032 mL
    50 mM 0.0609 mL 0.3045 mL 0.6090 mL 1.5225 mL
    60 mM 0.0508 mL 0.2538 mL 0.5075 mL 1.2688 mL
    80 mM 0.0381 mL 0.1903 mL 0.3806 mL 0.9516 mL
    100 mM 0.0305 mL 0.1523 mL 0.3045 mL 0.7613 mL
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    Macelignan Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Macelignan107534-93-0(+)-Anwulignan AnwuliganCOXCyclooxygenaseanti-inflammatoryanti-canceranti-diabetesneuroprotectivelignanhacatInhibitorinhibitorinhibit

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