Cat. No.: HY-N0064

Macelignan

(Synonyms: (+)-Anwulignan; Anwuligan)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Macelignan ((+)-Anwulignan; Anwuligan)?is an orally active lignan isolated from Myristica fragrans. Macelignan possesses many pharmacological activities, including anti-inflammatory, anti-cancer, anti-diabetes, and neuroprotective activities.

For research use only. We do not sell to patients.

Purity: 99.78%
CAS No.: 107534-93-0
Formula: C₂₀H₂₄O₄
Molecular Weight:328.40

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Macelignan Chemical Structure

CAS No.: 107534-93-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

COX-2

All COX Isoforms :

In Vitro

Macelignan (1-50 μM; 72 hours) does not reduce cell viability alone, however, UVB treatment, even at the lowest dose of 30 mJ/cm², reduces HaCaT cell viability in a dose-dependent mannner,it reduces approximately 80% of control values at 100 μM in hacat cells^[1].
Macelignan (0.1-1 μM; 24 hours) decreases COX-2 expression in a concentration-dependent manner,and at the highest concentration of macelignan (1 μM) , COX-2 expression is inhibited by almost 50% in hacat cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Hacat cells
Concentration:

1 μM; 2.5 μM; 5 μM; 10 μM; 15 μM; 50 μM
Incubation Time:

72 hours
Result:

Induced cell death by UVB irradiation at 30 mJ/cm² from 10 μM.

Western Blot Analysis^[1]

Cell Line:

Hacat cells
Concentration:

0.1 μM; 0.5 μM;1 μM
Incubation Time:

24 hours
Result:

Reduced UVB-induced COX-2 expression in cells.

In Vivo

Macelignan (oral administration; 15 mg/kg; every day for three weeks) exhibits?in vivo anti-diabetic effects. The baseline (day 0) fasting blood glucose levels does not differ between groups; at the end of the experiment, the values of the Macelignan-treated group are significantly lower compared to the diabetic control group in C57BL/KsJ-db/db?mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/KsJ-db/db mice^[2]
Dosage:

15 mg/kg
Administration:

Oral administration; 15 mg/kg; every day for three weeks
Result:

Significantly reduced the blood glucose levels in mice.

Chemical Information

CAS No. 107534-93-0
Appearance Solid
Molecular Weight 328.40
Formula C₂₀H₂₄O₄
Color White to off-white
SMILES

OC1=CC=C(C[C@H](C)[C@H](C)CC2=CC=C(OCO3)C3=C2)C=C1OC
Synonyms

(+)-Anwulignan; Anwuligan
Structure Classification
- Phenylpropanoids
- Lignans
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Proliferation/Viability AssayWBFlow CytometryCell Migration/Invasion AssayIn Vivo Efficacy Study

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Journal Impact Factor

Phytomedicine

Macelignan prevents colorectal cancer metastasis by inhibiting M2 macrophage polarization. [Abstract]

2024 Jan:122:155144. PMID: 37925889

Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144. [Abstract]

RAW264.7 cells and THP-1-derived macrophages were treated with Macelignan (0 - 80 μM) for 24 h, then CCK8 assay was performed to observed cell viability.

Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144. [Abstract]

Levels of CD206, Arg1, and CD163 were measured by WB treated with Macelignan (Mace) (2.5, 5, 10 μM).

Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144. [Abstract]

The proportion of CD206-positive cells was determined by flow cytometer. RAW264.7 cells and THP-1-derived macrophages were exposed to LPS with or without Macelignan (5 μM, 10 μM) for 24 h.

Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144. [Abstract]

Transwell assays were used to measure the migration and invasion of CRC cells treated with Macelignan (Mace) (5, 10 μM).

Macelignan purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:122:155144. [Abstract]

Tumor weight and volume treated with Macelignan (Mace) (5 mg/kg, p.o.).
Phytother Res

Macelignan Improves Functional Recovery After Spinal Cord Injury by Augmenting Autophagy via the AKT-mTOR-TFEB Signaling Pathway. [Abstract]

2025 May;39(5):2091-2109. PMID: 40062822
J Ethnopharmacol

Macelignan, a lignan from Myristica fragrans Houtt., rescues mitochondrial homeostasis and prevents cognitive decline in vascular dementia by modulating the mTOR-Mitophagy axis. [Abstract]

2025 Sep 12:120603. PMID: 40946820
Eur J Pharmacol

Macelignan mitigates oxidative and inflammatory damage in hypoxic neurons through PPARγ-dependent pathways: implications for Alzheimer's disease. [Abstract]

2026 Jan 20:1012:178483. PMID: 41419088
Bioorg Chem

Macelignan targets SLC7A11 to induce ferroptosis and modulate macrophage polarization via STAT6/PPAR-γ/KLF4 signaling. [Abstract]

2026 Feb:169:109422. PMID: 41456429
J Neuroimmunol

Macelignan inhibits the inflammatory response of microglia and regulates neuronal survival. [Abstract]

2020 Feb 15:339:577123. PMID: 31838278

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (304.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0451 mL

15.2253 mL

30.4507 mL

5 mM

0.6090 mL

3.0451 mL

6.0901 mL

10 mM

0.3045 mL

1.5225 mL

3.0451 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Anggakusuma, et al. Effects of macelignan isolated from Myristica fragrans Houtt. on UVB-induced matrix metalloproteinase-9 and cyclooxygenase-2 in HaCaT cells. J Dermatol Sci [Content Brief]

[2]. Jiyoung Yeo, et al. Effects of a multi-herbal extract on type 2 diabetes. Chin Med. 2011 Mar 4;6:10. [Content Brief]

[3]. Chun-Ai Cui, et al. Macelignan attenuates LPS-induced inflammation and reduces LPS-induced spatial learning impairments in rats. Neurosci Lett. 2008 Dec 19;448(1):110-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0451 mL	15.2253 mL	30.4507 mL	76.1267 mL
	5 mM	0.6090 mL	3.0451 mL	6.0901 mL	15.2253 mL
	10 mM	0.3045 mL	1.5225 mL	3.0451 mL	7.6127 mL
	15 mM	0.2030 mL	1.0150 mL	2.0300 mL	5.0751 mL
	20 mM	0.1523 mL	0.7613 mL	1.5225 mL	3.8063 mL
	25 mM	0.1218 mL	0.6090 mL	1.2180 mL	3.0451 mL
	30 mM	0.1015 mL	0.5075 mL	1.0150 mL	2.5376 mL
	40 mM	0.0761 mL	0.3806 mL	0.7613 mL	1.9032 mL
	50 mM	0.0609 mL	0.3045 mL	0.6090 mL	1.5225 mL
	60 mM	0.0508 mL	0.2538 mL	0.5075 mL	1.2688 mL
	80 mM	0.0381 mL	0.1903 mL	0.3806 mL	0.9516 mL
	100 mM	0.0305 mL	0.1523 mL	0.3045 mL	0.7613 mL

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Macelignan

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Publications Citing Use of MedChemExpress (MCE) Macelignan

All COX Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Macelignan Related Classifications

HY-N0064 Related Classifications