1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Menadione

Menadione  (Synonyms: Vitamin K3)

Cat. No.: HY-B0332 Purity: 99.53%
COA Handling Instructions

Menadione is a naphthoquinone that is converted into active vitamin K2 in the body. Menadione is a potential anticancer agent and radiosensitizer.

For research use only. We do not sell to patients.

Menadione Chemical Structure

Menadione Chemical Structure

CAS No. : 58-27-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
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Solid
500 mg USD 79 In-stock
1 g USD 119 In-stock
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Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of Menadione:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Menadione is a naphthoquinone that is converted into active vitamin K2 in the body. Menadione is a potential anticancer agent and radiosensitizer[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Menadione (0-100 μM, 24-72 h) induces necrosis at high concentrations (100 μM) and apoptosis at low concentrations (10-20 μM) in AR4-2J cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: AR4-2J cells
Concentration: 0-100 μM
Incubation Time: 24-72 h
Result: Inhibited cell proliferation in time- and dose-dependently manner at lower concentrations (1-20 μM).

Western Blot Analysis[2]

Cell Line: AR4-2J cells
Concentration: 0-100 μM
Incubation Time: 24 h
Result: Induced wild-type P53 at the dose of 1-20 μM.
Induced a low level of wild-type P53 at the dose of 100 μM.
In Vivo

Menadione (25-150 mg/kg, i.v., once time or every other day for five times) produces more severe lesionsin male Wister rats with a single injection than five injections [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wister rats[1]
Dosage: 25-150 mg/kg
Administration: i.v., once time or every other day for five times
Result: Showed microgranular degeneration of renal tubular cells at the dose of 25 mg/kg.
Observed microgranular degeneration of renal tubular cells and mild pulmonary hemorrhage at the dose of 50 mg/kg.
Produced lesions in the kidney, heart, liver and lung at the dose of 100-150 mg/kg.
Showed kidney apoptosis at the dose of 100-150 mg/kg.
Clinical Trial
Molecular Weight

172.18

Formula

C11H8O2

CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1C(C)=CC(C2=C1C=CC=C2)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (725.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 9.09 mg/mL (52.79 mM; Need ultrasonic)

H2O : 0.1 mg/mL (0.58 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.8079 mL 29.0394 mL 58.0788 mL
5 mM 1.1616 mL 5.8079 mL 11.6158 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (12.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (12.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.53%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 5.8079 mL 29.0394 mL 58.0788 mL 145.1969 mL
5 mM 1.1616 mL 5.8079 mL 11.6158 mL 29.0394 mL
10 mM 0.5808 mL 2.9039 mL 5.8079 mL 14.5197 mL
15 mM 0.3872 mL 1.9360 mL 3.8719 mL 9.6798 mL
20 mM 0.2904 mL 1.4520 mL 2.9039 mL 7.2598 mL
25 mM 0.2323 mL 1.1616 mL 2.3232 mL 5.8079 mL
30 mM 0.1936 mL 0.9680 mL 1.9360 mL 4.8399 mL
40 mM 0.1452 mL 0.7260 mL 1.4520 mL 3.6299 mL
50 mM 0.1162 mL 0.5808 mL 1.1616 mL 2.9039 mL
DMSO 60 mM 0.0968 mL 0.4840 mL 0.9680 mL 2.4199 mL
80 mM 0.0726 mL 0.3630 mL 0.7260 mL 1.8150 mL
100 mM 0.0581 mL 0.2904 mL 0.5808 mL 1.4520 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Menadione
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