1. Others
  2. Estrogen Receptor/ERR

PHTPP 

Cat. No.: HY-103456 Purity: 99.07%
Handling Instructions

PHTPP is a selective ERβ antagonist.

For research use only. We do not sell to patients.
PHTPP Chemical Structure

PHTPP Chemical Structure

CAS No. : 805239-56-9

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 92 In-stock
5 mg USD 84 In-stock
10 mg USD 144 In-stock
25 mg USD 312 In-stock
50 mg USD 552 In-stock
100 mg USD 912 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

PHTPP is a selective ERβ antagonist.

IC50 & Target

ERβ[1]

In Vitro

PHTPP is a selective ERβ antagonist. PHTPP reduces FSH-mediated cAMP production by 80% (p<0.01) while it has no effect on basal cAMP[1]. PHTPP (10-6 M) inhibits E2-stimulated ERβ activity, but does not suppress E2-stimulated ERα activity. A high dose of PHTPP (10-6 M) slightly increases class 1 Igf1 mRNA expression, and facilitates the DPN-induced increase in class 1 Igf1 mRNA expression[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.3623 mL 11.8117 mL 23.6233 mL
5 mM 0.4725 mL 2.3623 mL 4.7247 mL
10 mM 0.2362 mL 1.1812 mL 2.3623 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Cells are plated at a density of 3 to 4×105 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco’s Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37°C, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

423.31

Formula

C₂₀H₁₁F₆N₃O

CAS No.

805239-56-9

SMILES

OC1=CC=C(C2=C3N=C(C(F)(F)F)C=C(C(F)(F)F)N3N=C2C4=CC=CC=C4)C=C1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
PHTPP
Cat. No.:
HY-103456
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