Propyl pyrazole triol (Synonyms: PPT)

Name: Propyl pyrazole triol
Brand: MedChemExpress
SKU: HY-100689
Rating: 5.0 (18 reviews)

Cat. No.: HY-100689 Purity: 99.11%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Propyl pyrazole triol (PPT) is a selective estrogen receptor alpha (ERα) agonist. The relative binding affinity of Propyl pyrazole triol for ERα (ERα: 49%) around 410 times higher compared with estrogen receptor beta (ERβ: 0.12%).

For research use only. We do not sell to patients.

CAS No. : 263717-53-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 18 publication(s) in Google Scholar

Other Forms of Propyl pyrazole triol:

Propyl pyrazole triol (Standard) Get quote

18 Publications Citing Use of MCE Propyl pyrazole triol

Bio/Physico-chemical Assay

Flow Cytometry

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

: Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Feb 21;18(5):1961-1973.; Propyl pyrazole triol (PPT) (5 nM) promoted the expansion of CD4⁺CD25⁺ peritoneal regulatory T cells (Tregs).

: Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Feb 21;18(5):1961-1973.; Propyl pyrazole triol (PPT) (5 nM) notably increased the production of IL-10 and TGF-β1 in CD4⁺CD25⁺ peritoneal regulatory T cells (Tregs).

: Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]; ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) enhanced luciferase activity in MCF-7 cells.

: Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]; ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) induced proliferation of MCF-7 cells.

: Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]; ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) induced migration and invasion of MCF-7 cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

ERα

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	27.2 μM Compound: PPT	Cytotoxicity in hormone-dependent human MCF7 cells assessed as growth inhibition incubated for 4 day s by crystal violet assay Cytotoxicity in hormone-dependent human MCF7 cells assessed as growth inhibition incubated for 4 day s by crystal violet assay	[PMID: 23043242]
U2OS	EC₅₀	0.1 nM Compound: PPT	Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control	[PMID: 23043242]

In Vitro

The EC₅₀ of Propyl pyrazole triol for the ERα-dependent response is 140 pM in ERα-U2OS-Luc cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

386.44

Formula

C₂₄H₂₂N₂O₃

CAS No.

263717-53-9

Appearance

Solid

Color

White to off-white

SMILES

CCCC1=C(C2=CC=C(O)C=C2)N(C3=CC=C(O)C=C3)N=C1C4=CC=C(O)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (258.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5877 mL	12.9386 mL	25.8772 mL
5 mM	0.5175 mL	2.5877 mL	5.1754 mL
10 mM	0.2588 mL	1.2939 mL	2.5877 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.11%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (253 KB) RP-HPLC (332 KB) Elemental Analysis Report (111 KB)

Handling Instructions (2659 KB)

References

[1]. Influence of cellular ERalpha/ERbeta ratio on the ERalpha-agonist induced proliferation of human T47D breast cancer cells. [Content Brief]

[2]. Stauffer SR, et al. Pyrazole ligands: structure-affinity/activity relationships and estrogen receptor-alpha-selective agonists. J Med Chem. 2000 Dec 28;43(26):4934-47. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5877 mL	12.9386 mL	25.8772 mL	64.6931 mL
	5 mM	0.5175 mL	2.5877 mL	5.1754 mL	12.9386 mL
	10 mM	0.2588 mL	1.2939 mL	2.5877 mL	6.4693 mL
	15 mM	0.1725 mL	0.8626 mL	1.7251 mL	4.3129 mL
	20 mM	0.1294 mL	0.6469 mL	1.2939 mL	3.2347 mL
	25 mM	0.1035 mL	0.5175 mL	1.0351 mL	2.5877 mL
	30 mM	0.0863 mL	0.4313 mL	0.8626 mL	2.1564 mL
	40 mM	0.0647 mL	0.3235 mL	0.6469 mL	1.6173 mL
	50 mM	0.0518 mL	0.2588 mL	0.5175 mL	1.2939 mL
	60 mM	0.0431 mL	0.2156 mL	0.4313 mL	1.0782 mL
	80 mM	0.0323 mL	0.1617 mL	0.3235 mL	0.8087 mL
	100 mM	0.0259 mL	0.1294 mL	0.2588 mL	0.6469 mL

Propyl pyrazole triol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Propyl pyrazole triol263717-53-9PPTEstrogen Receptor/ERRInhibitorinhibitorinhibit

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Propyl pyrazole triol (Synonyms: PPT)

Customer Review

Other Forms of Propyl pyrazole triol:

Propyl pyrazole triol Related Antibodies

18 Publications Citing Use of MCE Propyl pyrazole triol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Estrogen Receptor/ERR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Propyl pyrazole triol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Propyl pyrazole triol Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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