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  3. Prazosin hydrochloride

Prazosin hydrochloride 

Cat. No.: HY-B0193A Purity: 99.93%
Handling Instructions

Prazosin hydrochloride is a well-tolerated, CNS-active α1-adrenergic receptor antagonist for the research of high blood pressure and alcohol use disorders. Prazosin hydrochloride potently inhibits Norepinephrine (NE)-stimulated 45Ca efflux with an IC50 of 0.15 nM.Prazosin hydrochloride inhibits organic cation transporters OCT-1 and OCT-3 with IC50s of 1.8, and 13 μM, respectively.

For research use only. We do not sell to patients.

Prazosin hydrochloride Chemical Structure

Prazosin hydrochloride Chemical Structure

CAS No. : 19237-84-4

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10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Prazosin hydrochloride:

Top Publications Citing Use of Products

    Prazosin hydrochloride purchased from MCE. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731.

    The expression of TNS1 in U937 and HL60 cells treated with different concentration of Prazosin is detected by Western blot analysis
    • Biological Activity

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    • Customer Review

    Description

    Prazosin hydrochloride is a well-tolerated, CNS-active α1-adrenergic receptor antagonist for the research of high blood pressure and alcohol use disorders[1]. Prazosin hydrochloride potently inhibits Norepinephrine (NE)-stimulated 45Ca efflux with an IC50 of 0.15 nM[2].Prazosin hydrochloride inhibits organic cation transporters OCT-1 and OCT-3 with IC50s of 1.8, and 13 μM, respectively[3].

    In Vitro

    Prazosin (0, 2.5, 5, 7.5, 10, 15, 20, 30, 40 and 50 µM) effectively inhibits the proliferation of U251 and U87 cells[4].
    Prazosin inhibits the migration and invasion of U251 and U87 cells[4].
    Prazosin treatment decreases the protein expression of components of the PI3K/AKT/mTOR signaling pathway. Prazosin (13.16 and 11.57 µM for U251 and U87 cells, 48 hours) decreases the expression levels of P70 and cyclin D1, which are downstream target genes of the PI3K/AKT/mTOR signaling pathway[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Proliferation Assay

    Cell Line: U251 and U87 cells
    Concentration: 0, 2.5, 5, 7.5, 10, 15, 20, 30, 40 and 50 µM.
    Incubation Time: 48 hours
    Result: The IC50s were 13.16±0.95 and 11.57±0.79 µM for U251 and U87 cells, respectively.

    Western Blot Analysis

    Cell Line: U251 and U87 cells
    Concentration: 13.16 and 11.57 µM for U251 and U87 cells, respectively
    Incubation Time: 48 hours
    Result: Protein expression levels of Bax and active Caspase-3 were increased.
    Bcl-2 levels were also decreased after prazosin treatment (P<0.05).
    The expression of p-AKT and p-mTOR, P70 and cyclin D1 were decreased.
    In Vivo

    Peripheral administration of Prazosin (0, 0.5, 1.0, 1.5 or 2.0 mg/kg; i.p.) can suppress not only central α1-adrenergic-mediated hyperexcitability but also stress-induced anxiety[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Fifty-five alcohol-naive male rats from the 60th generation of selective breeding for alcohol preference[1]
    Dosage: 0.5, 1.0, 1.5, or 2.0 mg/kg
    Administration: Injected intraperitoneally (IP); 0.5 mg/mL; once a day at 15 min prior to onset of the daily two hour two-bottle choice, alcohol versus water, access period for two consecutive days and then three weeks later for five consecutive days.
    Result: Significantly reduced alcohol intake during the initial two daily administrations, and this reduction of alcohol intake was maintained for five consecutive days by daily prazosin treatment in the subsequent more prolonged trial.
    Clinical Trial
    Molecular Weight

    419.86

    Formula

    C19H22ClN5O4

    CAS No.
    SMILES

    O=C(N1CCN(C2=NC(N)=C3C=C(OC)C(OC)=CC3=N2)CC1)C4=CC=CO4.Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 14.29 mg/mL (34.04 mM; Need ultrasonic)

    H2O : 0.59 mg/mL (1.41 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3817 mL 11.9087 mL 23.8175 mL
    5 mM 0.4763 mL 2.3817 mL 4.7635 mL
    10 mM 0.2382 mL 1.1909 mL 2.3817 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 1.43 mg/mL (3.41 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 1.43 mg/mL (3.41 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1.43 mg/mL (3.41 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
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    Product Name:
    Prazosin hydrochloride
    Cat. No.:
    HY-B0193A
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