Pyronaridine tetraphosphate

Name: Pyronaridine tetraphosphate
Brand: MedChemExpress
SKU: HY-14749A
Rating: 4.5 (2 reviews)

Cat. No.: HY-14749A Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pyronaridine tetraphosphate is an orally active Mannich base anti-malarial agent. Pyronaridine tetraphosphate is active against P. falciparum and Echinococcus granulosus infection.

For research use only. We do not sell to patients.

Pyronaridine tetraphosphate Chemical Structure

CAS No. : 76748-86-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 77	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 154	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 264	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Pyronaridine tetraphosphate:

Pyronaridine Get quote
Pyronaridine-d₄ tetraphosphate Get quote

2 Publications Citing Use of MCE Pyronaridine tetraphosphate

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Pyronaridine tetraphosphate is an orally active Mannich base anti-malarial agent. Pyronaridine tetraphosphate is active against P. falciparum and Echinococcus granulosus infection^[1]^[2].

IC₅₀ & Target

Plasmodium

In Vitro

Pyronaridine tetraphosphate (24 h) shows anti-P. falciparum activity with an IC₅₀ value of 1.53-3.94 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	P. falciparum: 3D7, K1, VS1, FCB, TM90C2a, FCR3
Concentration:	0-7 nM approximately
Incubation Time:	24 h
Result:	Exhibited anti-P. falciparum activity with Mean IC₅₀ values of 2.31 nM, 3.94 nM, 1.63 nM, 1.53 nM, 2.51 nM, 2.21 nM repectively.

In Vivo

Pyronaridine (57 mg/kg, oral administration, q.d. for 30 days) tetraphosphate reduces the parasitic burden in the Echinococcus granulosus-infected mice^[2].
Pyronaridine (57 mg/kg, intraperitoneal injection, q.d. for 3 days) tetraphosphate reduces the parasitic burden in secondarily infected (cysts) mice^[2].
Pyronaridine (57 mg/kg, intraperitoneal injection, for a single dose) tetraphosphate exhibits a higher exposure in the liver than in the plasma in male ICR mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Echinococcus granulosus-infected mice model^[2]
Dosage:	57 mg/kg
Administration:	Oral administration, q.d. for 30 days
Result:	Reduced 42.4% of parasite wet weight and killed 90.7% of secondary infection (cysts) of E. granulosus ss.

Clinical Trial

Molecular Weight

910.03

Formula

C₂₉H₄₄ClN₅O₁₈P₄

CAS No.

76748-86-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=C(CN2CCCC2)C=C(NC3=C4N=C(OC)C=CC4=NC5=CC(Cl)=CC=C53)C=C1CN6CCCC6.O=P(O)(O)O.O=P(O)(O)O.O=P(O)(O)O.O=P(O)(O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (27.47 mM; ultrasonic and warming and heat to 60°C)

DMSO : 12.5 mg/mL (13.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0989 mL	5.4943 mL	10.9886 mL
5 mM	0.2198 mL	1.0989 mL	2.1977 mL
10 mM	0.1099 mL	0.5494 mL	1.0989 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (54.94 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (255 KB) HNMR (322 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Vivas L, et al. Anti-malarial efficacy of pyronaridine and artesunate in combination in vitro and in vivo. Acta Trop. 2008 Mar;105(3):222-8. [Content Brief]

[2]. Jun Li, et al. Old drug repurposing for neglected disease: Pyronaridine as a promising candidate for the treatment of Echinococcus granulosus infections. EBioMedicine. 2020 Apr;54:102711. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.0989 mL	5.4943 mL	10.9886 mL	27.4716 mL
	5 mM	0.2198 mL	1.0989 mL	2.1977 mL	5.4943 mL
	10 mM	0.1099 mL	0.5494 mL	1.0989 mL	2.7472 mL
H₂O	15 mM	0.0733 mL	0.3663 mL	0.7326 mL	1.8314 mL
	20 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3736 mL
	25 mM	0.0440 mL	0.2198 mL	0.4395 mL	1.0989 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.