1. Metabolic Enzyme/Protease
    GPCR/G Protein
  2. Endogenous Metabolite
    LPL Receptor
  3. Sphingosine-1-phosphate

Sphingosine-1-phosphate (Synonyms: S1P)

Cat. No.: HY-108496 Purity: ≥98.0%
Handling Instructions

Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca2+ as an extracellular ligand for G protein-coupled receptors. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids.

For research use only. We do not sell to patients.

Sphingosine-1-phosphate Chemical Structure

Sphingosine-1-phosphate Chemical Structure

CAS No. : 26993-30-6

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Description

Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca2+ as an extracellular ligand for G protein-coupled receptors[1]. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids[2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

S1P (1 μM) induces a significant Ca2+ releases in HEK293 cells under serum starvation conditions (1% FCS)[1].
In a functional Ca2+ assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca2+ release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca2+ release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells[2].
In a functional Ca2+ assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC50 values for gpr3 (EC50=29 nM), gpr6 (EC50=15 nM) and gpr12 (EC50=24 nM), rat gpr3 (EC50=68 nM),respectively in HEK293 cells[2].
S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and Gqi5, which allows for phospholipase C stimulation by Gi proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC50s = 8 and 11 nM, respectively)[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

379.47

Formula

C18H38NO5P

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (263.53 mM; ultrasonic and warming and heat to 60°C)

Ethanol : 20 mg/mL (52.71 mM; ultrasonic and adjust pH to 3 with HCl)

H2O : 4 mg/mL (10.54 mM; ultrasonic and warming and adjust pH to >7.5 with 0.1 M Na)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6353 mL 13.1763 mL 26.3525 mL
5 mM 0.5271 mL 2.6353 mL 5.2705 mL
10 mM 0.2635 mL 1.3176 mL 2.6353 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2 mg/mL (5.27 mM); Clear solution

  • 5.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2 mg/mL (5.27 mM); Clear solution

  • 6.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 2 mg/mL (5.27 mM); Clear solution

*All of the co-solvents are available by MCE.
References

Purity: ≥98.0%

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Sphingosine-1-phosphate
Cat. No.:
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