1. TGF-beta/Smad
  2. TGF-β Receptor
  3. A 77-01

A 77-01 

Cat. No.: HY-78349 Purity: 99.92%
Handling Instructions

A 77-01 is a potent inhibitor of transforming growth factor (TGF)-β type I receptor superfamily activin-like kinase ALK5 with an IC50 of 25 nM.

For research use only. We do not sell to patients.

A 77-01 Chemical Structure

A 77-01 Chemical Structure

CAS No. : 607737-87-1

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 158 In-stock
Estimated Time of Arrival: December 31
5 mg USD 144 In-stock
Estimated Time of Arrival: December 31
10 mg USD 216 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

A 77-01 is a potent inhibitor of transforming growth factor (TGF)-β type I receptor superfamily activin-like kinase ALK5 with an IC50 of 25 nM[1].

IC50 & Target

IC50: 25 nM (ALK5)[1]

In Vitro

A-77-01 (0.01-10 µM; 25 hours; Mv1Lu cells) treatment effectively inhibits TGF-β-induced transcriptional activation in concentration-dependent fashion[1].
A-77-01 (1 µM; 24-72 hours; Mv1Lu cells) treatment efficiently prevents the growth-inhibitory effects of TGF-β[1].
A-77-01 (1 µM; 30 minutes; HaCaT cells) treatment inhibits TGF-β-induced phosphorylation of Smad2[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[1]

Cell Line: Mv1Lu cells
Concentration: 0.01 µM, 0.03 µM, 0.1 µM, 0.3 µM, 1 µM, 3 µM, 10 µM
Incubation Time: 25 hours
Result: Inhibited the luciferase activity induced by TGF-β in concentration-dependent fashion.

Cell Viability Assay[1]

Cell Line: Mv1Lu cells
Concentration: 1 µM
Incubation Time: 24 hours, 48 hours, 72 hours
Result: Efficiently prevented the growth-inhibitory effects of TGF-β.

Western Blot Analysis[1]

Cell Line: HaCaT cells
Concentration: 1 µM
Incubation Time: 30 minutes
Result: Inhibited TGF-β-induced phosphorylation of Smad2.
Molecular Weight

286.33

Formula

C₁₈H₁₄N₄

CAS No.

607737-87-1

SMILES

CC1=CC=CC(C2=NNC=C2C3=CC=NC4=CC=CC=C34)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (87.31 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4925 mL 17.4624 mL 34.9247 mL
5 mM 0.6985 mL 3.4925 mL 6.9849 mL
10 mM 0.3492 mL 1.7462 mL 3.4925 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

A 77-01TGF-β ReceptorTransforming growth factor beta receptorsInhibitorinhibitorinhibit

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Product Name:
A 77-01
Cat. No.:
HY-78349
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