Acesulfame
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Acesulfame is a synthetic sweetener. Long-term use of Acesulfame can affect cognitive function. Acesulfame potassium can suppress autophagic degradation of PD-L1 in RIL-175 and SK-Hep1 cells through the ERK1/2-mTORC1-ULK1 pathway, which may be related to immune evasion in cancer cells. Acesulfame can be used in research on neurological diseases, metabolic disorders, cancer, and immune evasion.
For research use only. We do not sell to patients.
- CAS No.: 33665-90-6
- Formula: C4H5NO4S
- Molecular Weight:163.15
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Acesulfame (1 mM, 24 hours) upregulates PD-L1 protein levels in RIL-175 and SK-Hep1 cells[2].
Acesulfame (1 mM, 24 hours) increases granzyme B production in RIL-175 and SK-Hep1 cells co-cultured with T cells[2].
Acesulfame (1 mM, 24 hours) activates ERK1/2-mTORC1-ULK1 pathway to suppress autophagic degradation of PD-L1 in RIL-175 and SK-Hep1 cells[2].
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Acesulfame (5-25 mM, 24 hours) inhibites mitochondrial metabolism in SH-SY5Y cells[4].
Acesulfame (5-25 mM, 24 hours) inhibites ATP production in cells and reduced phosphorylation of neuroprotective proteins, impaires energy metabolism and neuroprotective functions in SH-SY5Y cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RIL-175 (mouse hepatocellular carcinoma), SK-Hep1 (human hepatocellular carcinoma)
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Concentration:1 mM
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Incubation Time:24 hours
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Result:Significantly increased PD-L1 protein levels.
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Cell Line:SH-SY5Y (neuroblastoma cells)
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Concentration:5-25 mM
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Incubation Time:24 hours
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Result:Reduced the extracellular acidification rate (ECAR) in SH-SY5Y cells.
Acesulfame (12.5 mM, administered via drinking water for 40 weeks) affects the neurometabolic functions in C57BL/6J mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CD-1 mice (male and female)[3]
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Dosage:37.5 mg/kg
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Administration:Oral gavage (p.o.), once daily for 4 weeks
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Result:Increased body weight in male CD-1 mice.
Significantly altered the gut microbiome composition in both male and female mice.
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Animal Model:C57BL/6J mice[3]
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Dosage:12.5 mM
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Administration:Administered via drinking water, once daily for 40 weeks
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Result:Significant increase in fasting blood glucose and insulin levels in treated mice, without altering insulin sensitivity.
Chemical Information
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CAS No. 33665-90-6
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Appearance Solid
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Molecular Weight 163.15
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Formula C4H5NO4S
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Color White to off-white
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SMILES
O=C1C=C(C)OS(=O)(N1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Cong WN, et al. Long-term artificial sweetener acesulfame potassium treatment alters neurometabolic functions in C57BL/6J mice. PLoS One. 2013 Aug 7;8(8):e70257. [Content Brief]
[2]. Kim DH, et al. Acesulfame potassium upregulates PD-L1 in HCC cells by attenuating autophagic degradation. Biochem Biophys Res Commun. 2024 Jun 4;711:149921. [Content Brief]
[3]. Bian X, et al. The artificial sweetener acesulfame potassium affects the gut microbiome and body weight gain in CD-1 mice. PLoS One. 2017 Jun 8;12(6):e0178426. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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