Antibacterial agent 333 

Antibacterial agent 333, Tryptanthrin (HY-N6607) derivative, is a antibacterial agent with antibacterial activity against Vibrio parahaemolyticus (MIC₅₀ = 8 μg/mL) and Vibrio alginolyticus (MIC₅₀ = 16 μg/mL). Antibacterial agent 333 inhibits biofilm formation and maturation. Antibacterial agent 333 exhibits activity against drug-resistant Vibrio strains. Antibacterial agent 333 lacks cytotoxicity toward mammalian cells and has no hemolytic activity. Antibacterial agent 333 can be used for the research of infection^[1].

Antibacterial agent 333 (Compound 7h) potently inhibits the growth of Vibrio parahaemolyticus ATCC 17802 with an MIC of 8 μg/mL and inhibits Vibrio alginolyticus CICC 21664 with an MIC of 16 μg/mL^[1].
Antibacterial agent 333 (4-32 μg/mL) inhibits Vibrio parahaemolyticus biofilm formation^[1].
Antibacterial agent 333 (8-32 μg/mL; 0-12 h) exhibits rapid, concentration-dependent bactericidal activity against Vibrio parahaemolyticus, reducing bacterial counts below the detection limit within 6 h at 16 μg/mL (2×MIC) and within 4 h at 32 μg/mL (4×MIC)^[1].
Antibacterial agent 333 (16 μg/mL) disrupts the inner membrane permeability of Vibrio parahaemolyticus^[1].
Antibacterial agent 333 (4-32 μg/mL; 24-72 h) shows no cytotoxicity toward RAW 264.7 and HEK 293 T cells, with cell viability remaining above 80% even at the highest tested concentration^[1].
Antibacterial agent 333 (4-32 μg/mL) exhibits no hemolytic activity against sheep red blood cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

413.38

C₂₃H₁₅N₃O₅

O=C1C2=C(C=CC=C2)N=C3N1C(C=CC=C4)=C4C3(O)CC(C5=CC=C([N+]([O-])=O)C=C5)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yan BY, et al. Design, synthesis, and antibacterial activity evaluation of tryptanthrin derivatives. Bioorg Med Chem Lett. 2026;132:130503.  [Content Brief]