ERI1 Antibody (YA8261)
(Synonyms: 3 HEXO; 3'HEXO; HEXO; THEX1)ERI1 Antibody (YA8261) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ERI1.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
IHC-P
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Reactivity :
Human, Rat, Monkey
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:150-500 |
Product Details
ERI1 Antibody (YA8261) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ERI1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat, Monkey
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Calculated Molecular Weight Predicted band size: 39.9 kDa
Full length human recombinant protein of human ERI1 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ERI1 is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient 3'-end histone mRNA exonuclease activity and degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Required for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs. In vitro, does not have sequence specificity. In vitro, has weak DNA exonuclease activity. In vitro, shows biphasic kinetics such that there is rapid hydrolysis of the last three unpaired RNA nucleotides in the 39 flanking sequence followed by a much slower cleavage through the stem that occurs over a longer incubation period in the order of hours. ERI1-mediated RNA metabolism plays a key role in chondrogenesis[1][2][3][4][5].
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Subcellular Localization
Cytoplasm,Nucleus,Nucleus, nucleolus
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Isoforms & Post-Translational Modification
Q8IV48: 349 amino acids, molecular weight 40064 Da.
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Subunit
Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1
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SwissProt ID
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Synonyms
3 HEXO; 3'HEXO; HEXO; THEX1
Documentation
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