SHMT2 Antibody (YA7793)

Cat. No.: HY-P88109: Fiche technique User Guide for Antibodies
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SHMT2 Antibody (YA7793) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SHMT2.

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Size	Prix	Stock
20 μL		Demandez un devis et temps de livraison
50 μL		Demandez un devis et temps de livraison
100 μL		Demandez un devis et temps de livraison

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

SHMT2 Antibody (YA7793) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SHMT2.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P34897

Immunogen

Full length human recombinant protein of human SHMT2 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureté	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:25619277, PubMed:29364879, PubMed:33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29364879, PubMed:29452640). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985)

Subcellular Localization：Mitochondrion,Mitochondrion matrix, mitochondrion nucleoid,Mitochondrion inner membrane,Cytoplasm,Nucleus

Isoforms & Post-Translational Modification：P34897 has three isomers: P34897-1: 55993 Da (predicted); P34897-2: 54863 Da (predicted); P34897-3: 53455 Da (predicted).
Succinylation at Lys-280 inhibits the hydroxymethyltransferase activity

Subunit：Homotetramer; in the presence of bound pyridoxal 5'-phosphate (PubMed:25619277, PubMed:29180469)

Synonyms

GLYA; HEL-S-51e; NEDCASB; SHMT

Documentation

User Guide for Antibodies (1077 KB)