TLR8 Antibody (YA7757)
(Synonyms: CD288)TLR8 Antibody (YA7757) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to TLR8.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:2000-2500 |
Product Details
TLR8 Antibody (YA7757) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to TLR8.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 119.6 kDa
Human recombinant protein fragment corresponding to amino acids 32-153 of human TLR8 produced in E.coli.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human lymph node using TLR8 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88073, 1/2000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen using TLR8 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88073, 1/2000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using TLR8 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88073, 1/2000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using TLR8 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88073, 1/2000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
TLR8 is an endosomal pattern-recognition receptor that detects RNA degradation products and activates innate immune signaling in human myeloid cells[1][2]. Mechanistically, RNase T2 processes single-stranded RNA into uridine and purine-2′,3′-cyclophosphate-terminated oligoribonucleotides, which engage two TLR8 ligand-binding pockets[1][2]. In monocytes, TLR8 and TLR7 activate distinct pathways during RNA virus infection, making isoform-specific analysis essential for antiviral and inflammatory research[3]. Disease studies show that TLR8 gain-of-function variants cause neutropenia, antibody deficiency, lymphoproliferation, and bone marrow failure, linking excessive TLR8 signaling to human immunopathology[4]. For experimental applications, selective synthetic agonists help distinguish TLR8 biology from TLR7 biology by separating target-cell selectivity and cytokine-induction profiles[5].- TLR8 links RNA degradation sensing to innate immune activation in human myeloid cells.- Isoform-specific assays distinguish TLR8 signaling from related TLR7 pathways.- Agonist tools support controlled studies of cytokine induction and immune-cell activation.
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Subcellular Localization
Endosome membrane
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Expression
Tissue_Specificity: Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells -
Isoforms & Post-Translational Modification
Q9NR97 has two isomers: Q9NR97-1: 119828 Da (predicted); Q9NR97-2: 121764 Da (predicted).
Ubiquitinated by RNF216; leading to degradation by the proteasome丨Proteolytic processing occurs in monocytes and monocyte-derived macrophages by both furin-like proprotein convertase and cathepsins (PubMed:25297876) -
Subunit
Homodimer (PubMed:23520111, PubMed:25599397, PubMed:26929371, PubMed:29155428)
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SwissProt ID
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Synonyms
CD288
Documentation
[1]. Greulich W, et al. TLR8 Is a Sensor of RNase T2 Degradation Products. Cell. 2019 Nov 27;179(6):1264-1275.e13. [Content Brief]
[2]. Tanji H, et al. Toll-like receptor 8 senses degradation products of single-stranded RNA. Nat Struct Mol Biol. 2015 Feb;22(2):109-15. [Content Brief]
[3]. de Marcken M, et al. TLR7 and TLR8 activate distinct pathways in monocytes during RNA virus infection. Sci Signal. 2019 Oct 29;12(605):eaaw1347. [Content Brief]
[4]. Aluri J, et al. Immunodeficiency and bone marrow failure with mosaic and germline TLR8 gain of function. Blood. 2021 May 6;137(18):2450-2462. [Content Brief]
[5]. Gorden KB, et al. Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8. J Immunol. 2005 Feb 1;174(3):1259-68. [Content Brief]
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