Art. -Nr.: HY-113592

ERK-IN-4

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

ERK-IN-4 is an ERK inhibitor binds preferentially to ERK2 with a K_d of 5 μM. ERK-IN-4 specificity inhibits ERK Rsk-1 and Elk-1 phosphorylation. ERK-IN-4 has little effect on ERK protein phosphorylation by its upstream activator MEK1/2.

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Reinheit: 98.30%
CAS. Nr.: 1049738-54-6
Formel: C₁₄H₁₇ClN₂O₃S
Molecular Weight:328.81

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

ERK-IN-4 Chemical Structure

CAS. Nr.: 1049738-54-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

ERK2

5 μM (Kd)

Alle ERK Isoform-spezifische Produkte anzeigen :

In Vitro

ERK-IN-4 (Compound 76; 10-75 µM; 10 days) completely inhibits cell proliferation, and decreases number of cell colonies^[1].
ERK-IN-4 (Compound 76; 100 µM) inhibits ERK-mediated phosphorylation of Rsk-1 on Thr573 and ERK-mediated Elk-1 phosphorylation in HeLa cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

HeLa, A549, or SUM-159 cells
Concentration:

10 µM, 20 µM, 30 µM, 40 µM, 150 µM for A549, or SUM-159 cells; 25 µM, 50 µM, 75 µM for HeLa cells
Incubation Time:

10 days
Result:

Completely inhibited cell proliferation.

Chemical Information

CAS. Nr. 1049738-54-6
Appearance Solid
Molecular Weight 328.81
Formel C₁₄H₁₇ClN₂O₃S
Color Off-white to yellow
SMILES

O=C(N(CCN)C/1=O)SC1=C/C2=CC=C(OCC)C=C2.[H]Cl
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (1)

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Adv Sci (Weinh)

ACE-mediated Glycosylation Stabilizes PSAP To Promote GPR37-dependent Macrophage-Nucleus Pulposus Cells Crosstalk and TGFβ Signaling in Alleviating Intervertebral Disc Degeneration. [Abstract]

2025 Oct 14:e10662. PMID: 41085015

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (76.03 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0413 mL

15.2064 mL

30.4127 mL

5 mM

0.6083 mL

3.0413 mL

6.0825 mL

10 mM

0.3041 mL

1.5206 mL

3.0413 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (7.60 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (7.60 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Chad N Hancock, et al. Identification of novel extracellular signal-regulated kinase docking domain inhibitors. J Med Chem. 2005 Jul 14;48(14):4586-95. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0413 mL	15.2064 mL	30.4127 mL	76.0318 mL
	5 mM	0.6083 mL	3.0413 mL	6.0825 mL	15.2064 mL
	10 mM	0.3041 mL	1.5206 mL	3.0413 mL	7.6032 mL
	15 mM	0.2028 mL	1.0138 mL	2.0275 mL	5.0688 mL
	20 mM	0.1521 mL	0.7603 mL	1.5206 mL	3.8016 mL
	25 mM	0.1217 mL	0.6083 mL	1.2165 mL	3.0413 mL
	30 mM	0.1014 mL	0.5069 mL	1.0138 mL	2.5344 mL
	40 mM	0.0760 mL	0.3802 mL	0.7603 mL	1.9008 mL
	50 mM	0.0608 mL	0.3041 mL	0.6083 mL	1.5206 mL
	60 mM	0.0507 mL	0.2534 mL	0.5069 mL	1.2672 mL

Keywords

ERK-IN-4

1 Cited Publications

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ERK-IN-4

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Publications Citing Use of MedChemExpress (MCE) ERK-IN-4

Alle ERK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ERK-IN-4 Related Classifications

HY-113592 Related Classifications