Aloperine
Based on 7 publication(s) in Google Scholar
Aloperine is an alkaloid in sophora plants such as Sophora alopecuroides L, which has shown anti-cancer, anti-inflammatory and anti-virus properties. Aloperine is widely used to treat patients with allergic contact dermatitis eczema and other skin inflammation in China. Aloperine induces apoptosis and autophagy in HL-60 cells.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 56293-29-9
- Formula: C15H24N2
- Molecular Weight:232.37
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Aloperine
More- Mol Biomed. 2023 Nov 17;4(1):42. [Abstract]
- Int J Mol Sci. 2024 Aug 21;25(16):9083. [Abstract]
- Int Immunopharmacol. 2025 Sep 23:166:115590. [Abstract]
- Am J Physiol Cell Physiol. 2023 Oct 1;325(4):C1058-C1072. [Abstract]
- Int Immunopharmacol. 2021 Aug:97:107720. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Res Sq. 2026 Apr 13.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Flow Cytometry
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WB
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RT-PCR
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | CC50 |
>200 μM
Compound: Aloperine
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Cytotoxicity against HEK293T cells assessed as reduction in cell viability
Cytotoxicity against HEK293T cells assessed as reduction in cell viability
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[PMID: 29494844] |
| MDCK | CC50 |
>80 μM
Compound: 1
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Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo assay
Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo assay
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[PMID: 25147619] |
| MDCK | CC50 |
>80 μM
Compound: 1
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Cytotoxicity in MDCK cells assessed as reduction of cell viability incubated for 2 days by CytoTox-Glo cytotoxicity assay
Cytotoxicity in MDCK cells assessed as reduction of cell viability incubated for 2 days by CytoTox-Glo cytotoxicity assay
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[PMID: 29152054] |
| MDCK | EC50 |
14.5 μM
Compound: 1
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Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay
Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay
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[PMID: 25147619] |
| MT4 | CC50 |
>86 μM
Compound: 1
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Cytotoxicity in human MT4 cells assessed as reduction of cell viability incubated for 3 days by CytoTox-Glo cytotoxicity assay
Cytotoxicity in human MT4 cells assessed as reduction of cell viability incubated for 3 days by CytoTox-Glo cytotoxicity assay
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[PMID: 29152054] |
| MT4 | CC50 |
>86.2 μM
Compound: 1
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Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 3 days by CellTiter-Glo luminescent assay
Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 3 days by CellTiter-Glo luminescent assay
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[PMID: 26985308] |
| MT4 | EC50 |
1.75 μM
Compound: 1
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Antiviral activity against HIV1 NL4-3 infected in human MT4 cells assessed as reduction in viral replication measured on day 3 post infection by Nano-Glo luciferase reporter gene assay
Antiviral activity against HIV1 NL4-3 infected in human MT4 cells assessed as reduction in viral replication measured on day 3 post infection by Nano-Glo luciferase reporter gene assay
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[PMID: 26985308] |
Aloperine (1-20 μM; 24 hours) gives growth-inhibitory IC50 values in cancer cells ranges from 0.04 to 1.36 mM, the IC50 values in HL-60, U937, K562, EC109, A549 and HepG2 cells are 0.04, 0.27, 0.36, 1.11, 1.18 and 1.36 mM,respectively[1]. Aloperine (1-20 μM; 24 hours) induces apoptosis and decreases bcl-2 expression in HL-60 cells[1]. Aloperine (20–100 μM; 18 hours) induces autophagy and formation of acidic vacuole in HL-60 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 56293-29-9
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Appearance Solid
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Molecular Weight 232.37
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Formula C15H24N2
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Color White to off-white
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SMILES
[H][C@]1(C2)[C@](CCCC3)([H])N3C[C@]2([H])[C@@]4([H])C(CCCN4)=C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Mol Biomed
Aloperine targets lysosomes to inhibit late autophagy and induces cell death through apoptosis and paraptosis in glioblastoma. [Abstract]2023 Nov 17;4(1):42. PMID: 37975957
Aloperine purchased from MedChemExpress. Usage Cited in: Mol Biomed. 2023 Nov 17;4(1):42. [Abstract]
Cell viability of GL261, U87, A172, and HA exposed to Aloperine (ALO) (0.25, 0.5, 0.75, 1 mM) for different concentrations and time courses by cck-8 test.
Aloperine purchased from MedChemExpress. Usage Cited in: Mol Biomed. 2023 Nov 17;4(1):42. [Abstract]
Clone forming ability of GL261, U87, and A172 single cell after Aloperine (ALO) (0.25, 0.5 mM) treatment by clone forming assay.
Aloperine purchased from MedChemExpress. Usage Cited in: Mol Biomed. 2023 Nov 17;4(1):42. [Abstract]
Cell cycle distribution of GL261 and U87 exposed to Aloperine (ALO) (0.125, 0.25 mM) by flow cytometry.
Aloperine purchased from MedChemExpress. Usage Cited in: Mol Biomed. 2023 Nov 17;4(1):42. [Abstract]
Expression of G1-phase cell cycle related proteins and cell cycle inhibitors with Aloperine (ALO) (0.25, 0.5, 1.0 mM) in GL261 and U87, tested by western blot.
Aloperine purchased from MedChemExpress. Usage Cited in: Mol Biomed. 2023 Nov 17;4(1):42. [Abstract]
Transcriptional expression of LC3B, after Aloperine (ALO) (0.125, 0.250 mM, 24 h) treatment in GL261 tested by qPCR.
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Int J Mol Sci
2024 Aug 21;25(16):9083. PMID: 39201769 -
Int Immunopharmacol
Aloperine exerts anti-tumor effect and activates the tumor cell-intrinsic STING pathway in gallbladder cancer. [Abstract]2025 Sep 23:166:115590. PMID: 40991999 -
Am J Physiol Cell Physiol
Aloperine Protects Pulmonary Hypertension via Triggering PPARγ Signaling and Inhibiting Calcium Regulatory Pathway in Pulmonary Arterial Smooth Muscle Cells. [Abstract]2023 Oct 1;325(4):C1058-C1072. PMID: 37661916 -
Int Immunopharmacol
Aloperine improves osteoporosis in ovariectomized mice by inhibiting RANKL-induced NF-κB, ERK and JNK approaches. [Abstract]2021 Aug:97:107720. PMID: 33945918 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Solvent & Solubility
DMSO : 5.56 mg/mL (23.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Lin Z, et al. In vitro anti-tumour activities of quinolizidine alkaloids derived from Sophora flavescens Ait. Basic Clin Pharmacol Toxicol. 2011 May;108(5):304-9. [Content Brief]
[2]. Yuan XY, et al. Effects and mechanisms of aloperine on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice. Eur J Pharmacol. 2010 Mar 10;629(1-3):147-52. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3035 mL | 21.5173 mL | 43.0346 mL | 107.5866 mL |
| 5 mM | 0.8607 mL | 4.3035 mL | 8.6069 mL | 21.5173 mL | |
| 10 mM | 0.4303 mL | 2.1517 mL | 4.3035 mL | 10.7587 mL | |
| 15 mM | 0.2869 mL | 1.4345 mL | 2.8690 mL | 7.1724 mL | |
| 20 mM | 0.2152 mL | 1.0759 mL | 2.1517 mL | 5.3793 mL |
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