2. NF-κB Immunology/Inflammation
  3. NF-κB Interleukin Related
  4. Bengamide B

Bengamide B is an alkaloid with anti-tumor and anti-inflammatory activities. Bengamide B reduces the phosphorylation level of IκBα, thereby blocking the activation of NF-κB. Bengamide B inhibits the proliferation of tumor cells. Bengamide B can be used in research related to inflammatory diseases and cancers.

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Bengamide B

Bengamide B Chemical Structure

CAS No. : 104947-69-5

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Bengamide B Related Antibodies

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Description

Bengamide B is an alkaloid with anti-tumor and anti-inflammatory activities. Bengamide B reduces the phosphorylation level of IκBα, thereby blocking the activation of NF-κB. Bengamide B inhibits the proliferation of tumor cells. Bengamide B can be used in research related to inflammatory diseases and cancers[1][2].

IC50 & Target[1]

IL-6

 

Cellular Effect
Cell Line Type Value Description References
HCT-116 IC50
0.002 μM
Compound: 7
Cytotoxicity against human HCT116 cells after 48 hrs by SRB assay
Cytotoxicity against human HCT116 cells after 48 hrs by SRB assay
[PMID: 22705020]
MDA-MB-435 IC50
2 nM
Compound: Bengamide B
Cytotoxicity against human MDA-MB-435 cells assessed as reduction in cell viability after 72 hrs by MTS assay
Cytotoxicity against human MDA-MB-435 cells assessed as reduction in cell viability after 72 hrs by MTS assay
[PMID: 28185457]
RAW264.7 IC50
0.085 μM
Compound: 7
Inhibition of LPS-stimulated NFkappaB activation transfected in mouse RAW264.7 cells after 18 hrs by luciferase reporter gene assay
Inhibition of LPS-stimulated NFkappaB activation transfected in mouse RAW264.7 cells after 18 hrs by luciferase reporter gene assay
[PMID: 22705020]
RAW264.7 IC50
0.5 μM
Compound: 7
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated IL-6 expression up to 10 uM by quantitative RT-PCR analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated IL-6 expression up to 10 uM by quantitative RT-PCR analysis
[PMID: 22705020]
RAW264.7 IC50
0.5 μM
Compound: 7
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated MCP1 expression up to 10 uM by quantitative RT-PCR analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated MCP1 expression up to 10 uM by quantitative RT-PCR analysis
[PMID: 22705020]
RAW264.7 IC50
0.5 μM
Compound: 7
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated TNFalpha expression up to 10 uM by quantitative RT-PCR analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated TNFalpha expression up to 10 uM by quantitative RT-PCR analysis
[PMID: 22705020]
RAW264.7 IC50
> 50 μM
Compound: 7
Cytotoxicity against mouse RAW264.7 cells after 18 hrs by MTT assay
Cytotoxicity against mouse RAW264.7 cells after 18 hrs by MTT assay
[PMID: 22705020]
In Vitro

Bengamide B (18 h) potently inhibits NF-κB activity in RAW264.7 cells with an IC50 of 0.085 μM; it shows no cytotoxicity against RAW264.7 cells at concentrations up to 50 μM; it also potently inhibits the growth of HCT-116 cells with an IC50 of 0.002 μM[1].
Bengamide B (0-10 μM; 4 h) dose-dependently inhibits LPS (HY-D1056)-induced phosphorylation of IκBα in RAW264.7 cells[1].
Bengamide B (0.01-10 μM) dose-dependently inhibits LPS-induced expression of TNFα, IL-6 and MCP-1 in RAW264.7 cells[1].
Bengamide B (3 days) potently inhibits the proliferation of MDA-MB-435 human breast cancer cells in vitro, with an IC50 value of 0.0024 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bengamide B Related Antibodies

Western Blot Analysis[1]

Cell Line: murine RAW264.7 macrophage cells
Concentration: 0, 0.01, 0.1, 1, 10 μM
Incubation Time: 4 h
Result: Dose-dependently reduced the level of phosphorylated IκBα, with greater inhibitory activity observed at higher concentrations.
Maintained consistent total IκBα and β-actin levels across concentrations.
In Vivo

Bengamide B (10-33 µmol/kg; intravenous injection; three times per week; for 3 consecutive weeks) exerts statistically significant, dose-dependent inhibitory effects on the growth of MDA-MB-435 breast cancer xenografts in athymic nude mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic (nu/nu) nude mice treated MDA-MB-435 cells[2]
Dosage: 10 µmol/kg; 33 µmol/kg (20 mg/kg)
Administration: i.v.; three times per week; 3 weeks
Result: Produced a 45% T/C value, with a 0.5% change in average body weight and 0/8 mice dead at 10 µmol/kg.
Produced a 31% T/C value, with a -2.3% change in average body weight and 0/8 mice dead at 33 µmol/kg.
Induced statistically significant tumor growth inhibition at both doses.
Molecular Weight

598.81

Formula

C32H58N2O8
CAS No.
SMILES

CC(C)/C=C/[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC)C(N[C@H]1CC[C@H](OC(CCCCCCCCCCCCC)=O)CN(C)C1=O)=O
Structure Classification
Initial Source

Jaspis digonoxea
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Bengamide B Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bengamide B104947-69-5NF-κBInterleukin RelatedNuclear factor-κBNuclear factor-kappaBILHCT-116 cellsMDA-MB-435 human breast carcinoma cellsinflammatory diseasesIκBαTNFαathymic nude miceMCP-1IL-6RAW264.7 cellsInhibitorinhibitorinhibit

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