1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme
  3. BI8622

BI8622 

Cat. No.: HY-120929 Purity: 99.35%
Handling Instructions

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 3.1 μM.

For research use only. We do not sell to patients.

BI8622 Chemical Structure

BI8622 Chemical Structure

CAS No. : 1875036-74-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 385 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 550 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1650 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2250 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    BI8622 purchased from MCE. Usage Cited in: Cell Death Differ. 2020 Dec;27(12):3273-3288.

    Protein expression of Myst2 in Huwe1 inhibits mESCs is analyzed by Western blot using Huwe1 inhibitor (BI8622). Indicated amounts of BI8622 is treated for 24 h to inhibit Huwe1.

    BI8622 purchased from MCE. Usage Cited in: J Clin Invest. 2020 Dec 1;130(12):6301-6316.

    Immunoblot analysis of pro–caspase-1 and its subunit p20. Caspase-1 activation in NLRP3, AIM2, and NLRC4 inflammasomes is substantially reduced in BMDMs treated with BI8622 (10 μM) compared with untreated BMDMs.
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    Description

    BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 3.1 μM[1].

    IC50 & Target

    IC50: 3.1 μM (HUWE1)[1]

    In Vitro

    BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC50 value of 6.8 μM in HeLa cells[1].
    BI8622 suppresses colony formation of Ls174T cells with estimated IC50 value of 8.4 μM[1].
    BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1[1].
    BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells[1].
    BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Cycle Analysis[1]

    Cell Line: Ls174T cells
    Concentration: 0 μM, 5 μM,10 μM, 15 μM, 20 μM
    Incubation Time: 0-4 days
    Result: Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.

    Western Blot Analysis[1]

    Cell Line: HeLa cells
    Concentration: 0 μM, 10 μM, 20 μM
    Incubation Time: 16 hours
    Result: Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.
    Molecular Weight

    426.51

    Formula

    C₂₅H₂₆N₆O

    CAS No.

    1875036-74-0

    SMILES

    O=C(C1=NC=NC(N2CCC(C3=CC=CC=C3)(C#N)CC2)=C1C)NC4=CC=C(CN)C=C4

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (293.08 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3446 mL 11.7231 mL 23.4461 mL
    5 mM 0.4689 mL 2.3446 mL 4.6892 mL
    10 mM 0.2345 mL 1.1723 mL 2.3446 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (4.88 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (4.88 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (4.88 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    BI8622BI 8622BI-8622E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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    Product Name:
    BI8622
    Cat. No.:
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