1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme
  3. BI8622

BI8622 

Cat. No.: HY-120929
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BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 3.1 μM.

For research use only. We do not sell to patients.

BI8622 Chemical Structure

BI8622 Chemical Structure

CAS No. : 1875036-74-0

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Description

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 3.1 μM[1].

IC50 & Target

IC50: 3.1 μM (HUWE1)[1]

In Vitro

BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC50 value of 6.8 μM in HeLa cells[1].
BI8622 suppresses colony formation of Ls174T cells with estimated IC50 value of 8.4 μM[1].
BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1[1].
BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells[1].
BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells[1].

Cell Cycle Analysis[1]

Cell Line: Ls174T cells
Concentration: 0 μM, 5 μM,10 μM, 15 μM, 20 μM
Incubation Time: 0-4 days
Result: Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0 μM, 10 μM, 20 μM
Incubation Time: 16 hours
Result: Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.
Molecular Weight

426.51

Formula

C₂₅H₂₆N₆O

CAS No.

1875036-74-0

SMILES

O=C(C1=NC=NC(N2CCC(C3=CC=CC=C3)(C#N)CC2)=C1C)NC4=CC=C(CN)C=C4

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Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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BI8622
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