2. Metabolic Enzyme/Protease
    Apoptosis
  3. E1/E2/E3 Enzyme
    Apoptosis
  4. NSC697923

NSC697923 

Cat. No.: HY-13811 Purity: 99.16%
COA Handling Instructions

NSC697923 is a potent UBE2N (ubiquitin-conjugating enzyme E2 N, Ubc13) inhibitor. NSC697923 induces neuroblastoma (NB) cell death via promoting nuclear importation of p53 in p53 wild-type NB cells. NSC697923 also induces cell death in p53 mutant NB cells by activation of JNK-mediated apoptotic pathway. NSC697923 inhibits DNA damage and NF-κB signaling. Antitumor activity.

For research use only. We do not sell to patients.

NSC697923 Chemical Structure

NSC697923 Chemical Structure

CAS No. : 343351-67-7

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Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 80 In-stock
Estimated Time of Arrival: December 31
25 mg USD 150 In-stock
Estimated Time of Arrival: December 31
50 mg USD 250 In-stock
Estimated Time of Arrival: December 31
100 mg USD 400 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE NSC697923

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

NSC697923 is a potent UBE2N (ubiquitin-conjugating enzyme E2 N, Ubc13) inhibitor. NSC697923 induces neuroblastoma (NB) cell death via promoting nuclear importation of p53 in p53 wild-type NB cells. NSC697923 also induces cell death in p53 mutant NB cells by activation of JNK-mediated apoptotic pathway. NSC697923 inhibits DNA damage and NF-κB signaling. Antitumor activity[1][2].

In Vitro

NSC697923 (0-5 μM; 24 hours) shows cytotoxic effect on NB cell lines[1].
NSC697923 (3 μM; 2 hours) can also induce apoptosis in p53 mutant NB cells by activation of JNK-mediated apoptotic pathway[1].
NSC697923 induces apoptosis in p53 wild-type NB cell lines by promoting p53 nuclear translocation and activation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Three MYCN-amplified cell lines: IMR32, NGP, NB19 and three MYCN-non-amplified cell lines: CHLA-255, SK-N-AS, and SH-SY5Y
Concentration: 0-5 μM
Incubation Time: 24 hours
Result: Significantly reduced NB cells viability in a dose-dependent manner. Also induced cell death in the p53 non-functional cell line SK-N-AS and p53 partially functional cell line NB-19.

Western Blot Analysis[1]

Cell Line: p53 wild-type SH-SY5Y and IMR32 cells
Concentration: 3 μM
Incubation Time: 2 hours
Result: Induced expression of p53-targeted gene p21 as well as the cleavage of caspase 3 in two p53 wild-type cell lines SH-SY5Y and IMR32.
In Vivo

NSC697923 (5 mg/kg; i.p.; daily for 10 days) suppresses NB tumor growth in SH-SY5Y and NGP xenografts[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5- to 6-week-old female athymic Ncr nude mice (orthotopic mouse model of NB; SH-SY5Y and NGP xenografts)[1]
Dosage: 5 mg/kg
Administration: I.p.; daily for 10 days
Result: Significant tumor regression in both SH-SY5Y and NGP xenografts.
Molecular Weight

267.26

Appearance

Solid

Formula

C11H9NO5S
CAS No.
SMILES

O=S(C1=CC=C([N+]([O-])=O)O1)(C2=CC=C(C)C=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (748.33 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7417 mL 18.7084 mL 37.4167 mL
5 mM 0.7483 mL 3.7417 mL 7.4833 mL
10 mM 0.3742 mL 1.8708 mL 3.7417 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 5 mg/mL (18.71 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 5 mg/mL (18.71 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.16%

COA (250 KB) HNMR (110 KB) RP-HPLC (198 KB) MS (109 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

NSC697923343351-67-7NSC 697923NSC-697923E1/E2/E3 EnzymeApoptosisE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligasenuclearimportationp53neuroblastomacellsantitumorDNAdamageInhibitorinhibitorinhibit

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