1. Metabolic Enzyme/Protease
  2. E1/E2/E3 Enzyme
  3. BI8626

BI8626 

Cat. No.: HY-120204
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BI8626 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 0.9 μM.

For research use only. We do not sell to patients.

BI8626 Chemical Structure

BI8626 Chemical Structure

CAS No. : 1875036-75-1

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Description

BI8626 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC50 of 0.9 μM[1].

IC50 & Target

IC50: 0.9 μM (HUWE1)[1]

In Vitro

BI8626 induces HUWE1 ectopically expresses to abolishe ubiquitination of MCL1 in HeLa cells[1].
BI8626 suppresses colony formation of Ls174T cells with estimated IC50 value of 0.7 μM, and BI8622 (1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1[1].
BI8626 (0-50 μM; 0-6 hours) retards the degradation of MCL1 in response to UV irradiation to the same extent as depletion of HUWE1 in U2OS cells[1].

Cell Cycle Analysis[1]

Cell Line: Ls174T cells
Concentration: 0 μM, 5 μM,10 μM, 15 μM, 20 μM
Incubation Time: 0-4 days
Result: Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.

Western Blot Analysis[1]

Cell Line: U2OS cells
Concentration: 0 μM, 20 μM, 50 μM
Incubation Time: 0 hour,1 hour,2 hours,4 hours,6 hours
Result: Retarded the degradation of MCL1 in response to UV irradiation in HeLa cells by inhibiting HUWE1 in U2OS cells.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Molecular Weight

440.54

Formula

C₂₅H₂₈N₈

CAS No.

1875036-75-1

SMILES

NCC1=CC=CC(CNC2=C(C3=NC=N2)N=CN=C3N4CCN(CC5=CC=CC=C5)CC4)=C1

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Room temperature in continental US; may vary elsewhere

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BI8626
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BI8626

Cat. No.: HY-120204