Zevontabart
Based on 1 Customer Validation
Zevontabart (MYTX-011 Antibody; Q-397) is a pH-dependent anti-c-MET antibody. Zevontabart regulates the trafficking process of c-MET to reduce receptor recycling, and enhances its own endocytosis and accumulation in c-MET-expressing cells. Zevontabart induces cytotoxicity in solid tumor cells and exerts anti-tumor activity in a non-small cell lung cancer xenograft mouse model. Zevontabart can be used to synthesize ADC, such as: MYTX-011. Zevontabart can be used in studies related to non-small cell lung cancer, with its corresponding isotype control being Human IgG1 kappa, Isotype Control (HY-P99001).
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- Pureté: 99.46%
- CAS No.: 3060218-90-5
- Masse moléculaire:143.999 kDa
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
In Vitro
Zevontabart (50 nM; 120 s association, 300 s dissociation) binds to recombinant human c-MET in a pH-dependent manner, with a faster dissociation rate at the endolysosomal pH of 5.4 compared to the neutral pH of 7.4[1].
Zevontabart (10 nM; 0.5-3 h) accumulates highly in lysosomes of NCI-H1975 c-MET+ non-small cell lung cancer (NSCLC) cells[1].
Zevontabart (25 nM; 30 min) shows low colocalization with Rab4+ rapidly recycling endosomes in EBC-1 c-MET+ cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NCI-H1975 (c-MET+ NSCLC) cells transfected with LAMP1-RFP (lysosomal marker)
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Concentration:10 nmol/L
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Incubation Time:30 min, 1 h, 3 h
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Result:Showed equivalent colocalization with LAMP1-RFP+ lysosomes to the parent mAb at all time points, but exhibited significantly higher fluorescence intensity within lysosomes relative to the parent mAb.
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Cell Line:EBC-1 (c-MET+ cancer) cells transfected with Rab4-mRuby (fast-recycling endosome marker)
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Concentration:25 nmol/L
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Incubation Time:30 min
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Result:Showed significantly lower colocalization with Rab4-mRuby+ fast-recycling endosomes relative to the parent mAb.
Parmacokinetics
| Species | Dose | Route | T1/2 |
|---|---|---|---|
| Cynomolgus Monkey[1] | 2.7 mg/kg | i.v. | 158 |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID mice (6-8-week-old female; non-small cell lung cancer xenograft model)[1]
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Dosage:0.5 mg/kg; 1 mg/kg; 2 mg/kg; 4 mg/kg; 6 mg/kg
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Administration:i.v.; single dose
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Result:Significantly reduced tumor size and more effectively slowed tumor growth.
Gene ID
Accession
Target
HGFR/c-Met
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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IgG1-kappa
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized HGFR Protein, Human (HEK293, HY-P78490) can bind Zevontabart. The ED50 for this effect is 88.01 ng/mL.
Chemical Information
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CAS No. 3060218-90-5
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Appearance Liquid
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Masse moléculaire 143.999 kDa
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Color Colorless to light yellow
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SMILES
[Zevontabart]
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Synonyms
MYTX-011 Antibody; Q-397
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Livraison
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)