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  3. Bacterial Antibiotic
  4. Cefiderocol

Cefiderocol (S-649266) is a siderophore cephalosporin which has a potent activity against a broad range of aerobic Gram-negative bacterial species with MIC50s of 2 μg/mL or less.

For research use only. We do not sell to patients.

CAS No. : 1225208-94-5

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Customer Review

Based on 20 publication(s) in Google Scholar

Other Forms of Cefiderocol:

Cefiderocol Related Antibodies

Top Publications Citing Use of Products

    Cefiderocol purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2026 Mar 4;70(3):e0126325.  [Abstract]

    Expected in vitro PD effect with different infusion durations in the Ax114 strain and Ax130 strain. Simulations were performed using body weight-based dosing of Ceftazidime/Avibactam (50/12.5 mg/kg, capped at 2,000/500 mg) and Cefiderocol (60 mg/kg, capped at 2,000 mg) administered every 8 h. Solid lines represent the median predicted bacterial response, and shaded areas indicate the 95% confidence interval based on the MBM simulations.

    Cefiderocol purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2026 Mar 4;70(3):e0126325.  [Abstract]

    CFU reduction (log10 CFU/mL) with Ceftazidime/Avibactam and Cefiderocol as monotherapies (blue) and in combination (red), compared to the growth control (green). Data are shown for Ax114 (small circles) and Ax130 (larger circles with grid), highlighting differential responses across strains and treatments.

    Cefiderocol purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2025 Dec 5:e0084225.  [Abstract]

    Activity of Aztreonam-avibactam, Cefiderocol, Cefepime-taniborbactam, Tigecycline, and Colistin against MBL-producing Enterobacterales stratified by country, organism, and MBL type.

    Cefiderocol purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2024 Feb 7;68(2):e0133223.  [Abstract]

    Minimum inhibitory concentrations (MICs) of different β-lactams against E. coli DH10B strains harboring the pHSG298 blaNDM-1(K224X) gene. The heat map represents the doubling dilution changes in the MIC values of the K224X variants compared with that of the NDM-1 wild type. The results shown are the mode of three biological replicates. FEP, cefepime; CAZ, ceftazidime; IMI, imipenem; TBPM, tebipenem; FDC, Cefiderocol; WT, wild type.

    Cefiderocol purchased from MedChemExpress. Usage Cited in: Antibiotics (Basel). 2023 Jul 20;12(7):1212.

    Distribution of Cefiderocol MICs determined with the standard BMD method according to the acquired carbapenem-resistance marker(s).

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Cefiderocol (S-649266) is a siderophore cephalosporin which has a potent activity against a broad range of aerobic Gram-negative bacterial species with MIC50s of 2 μg/mL or less.

    IC50 & Target

    β-lactam

     

    In Vitro

    Cefiderocol (S-649266), a novel parenteral siderophore cephalosporin conjugated with a catechol moiety, has a characteristic antibacterial spectrum with a potent activity against a broad range of aerobic Gram-negative bacterial species, including carbapenem-resistant strains of Enterobacteriaceae and nonfermenting bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii. Cefiderocol has affinity mainly for PBP3 of Enterobacteriaceae and nonfermenting bacteria similar to that of GR20263. A deficiency of the iron transporter PiuA in P. aeruginosa or both CirA and Fiu in Escherichia coli can cause 16-fold increases in cefiderocol MICs, suggesting that these iron transporters contribute to the permeation of cefiderocol across the outer membrane. The deficiency of OmpK35/36 in Klebsiella pneumoniae and the overproduction of efflux pump MexA-MexB-OprM in P. aeruginosa show no significant impact on the activity of cefiderocol[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cefiderocol Related Antibodies
    Clinical Trial
    Molecular Weight

    752.21

    Formula

    C30H34ClN7O10S2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C(N12)=C(C[N+]3(CCNC(C4=CC=C(O)C(O)=C4Cl)=O)CCCC3)CS[C@]2([H])[C@H](NC(/C(C5=CSC(N)=N5)=N\OC(C)(C(O)=O)C)=O)C1=O)[O-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, stored under nitrogen

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (132.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 1.06 mg/mL (1.41 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3294 mL 6.6471 mL 13.2942 mL
    5 mM 0.2659 mL 1.3294 mL 2.6588 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (2.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (2.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *The compound is unstable in solutions, freshly prepared is recommended.

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.60%

    SDS (393 KB)

    COA (262 KB) HNMR (335 KB) RP-HPLC (254 KB) LCMS (232 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    For the determination of cefiderocol MIC, iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) is prepared, except for the cases that are required to determine MICs under specific conditions. The quality control MIC ranges of cefiderocol are 0.06 to 0.5 μg/mL for both E. coli ATCC 25922 and P. aeruginosa ATCC 27853. For anaerobic bacteria, brucella agar supplemented with hemin, vitamin K1, and laked sheep blood is used[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.3294 mL 6.6471 mL 13.2942 mL 33.2354 mL
    DMSO 5 mM 0.2659 mL 1.3294 mL 2.6588 mL 6.6471 mL
    10 mM 0.1329 mL 0.6647 mL 1.3294 mL 3.3235 mL
    15 mM 0.0886 mL 0.4431 mL 0.8863 mL 2.2157 mL
    20 mM 0.0665 mL 0.3324 mL 0.6647 mL 1.6618 mL
    25 mM 0.0532 mL 0.2659 mL 0.5318 mL 1.3294 mL
    30 mM 0.0443 mL 0.2216 mL 0.4431 mL 1.1078 mL
    40 mM 0.0332 mL 0.1662 mL 0.3324 mL 0.8309 mL
    50 mM 0.0266 mL 0.1329 mL 0.2659 mL 0.6647 mL
    60 mM 0.0222 mL 0.1108 mL 0.2216 mL 0.5539 mL
    80 mM 0.0166 mL 0.0831 mL 0.1662 mL 0.4154 mL
    100 mM 0.0133 mL 0.0665 mL 0.1329 mL 0.3324 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Cefiderocol Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cefiderocol1225208-94-5S-649266S649266S 649266BacterialAntibioticInhibitorinhibitorinhibit

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