Art. -Nr.: HY-N0766

Isorhynchophylline

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

Isorhynchophylline is an alkaloid isolated from Crocus sativus with anticancer, antihypertensive, anti-inflammatory, and neuroprotective properties. Isorhynchophylline can be applied to cardiovascular and neurological disorders, and cancer studies.

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Reinheit: 99.82%
CAS. Nr.: 6859-01-4
Formel: C₂₂H₂₈N₂O₄
Molecular Weight:384.47

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Isorhynchophylline Chemical Structure

CAS. Nr.: 6859-01-4

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

Alle NF-κB Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	>40 μM Compound: 6	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 21070010]
Microglia	IC₅₀	19 μM Compound: 6	Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs	[PMID: 18588343]
SW480	IC₅₀	>40 μM Compound: 6	Cytotoxicity against human SW480 cells after 48 hrs by MTT assay Cytotoxicity against human SW480 cells after 48 hrs by MTT assay	[PMID: 21070010]

In Vitro

Isorhynchophylline (0.0625-40 µM, 48 h) shows cytotoxicity against human HL60 cells and SW480 cells (MTT assay), with IC₅₀s greater than 40 μM^[1].
Isorhynchophylline (3-15 µg/mL, 48 h) inhibits LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation^[2].
Isorhynchophylline (0.1-10.0 μM, 24 h) effectively inhibits angiotensin II-induced proliferation in rat vascular smooth muscle cells, at least in part, due to increased NO production and regulation of the cell cycle^[3].
Isorhynchophylline (0-300 μM, 48 h) exerts anticancer and anti-metastatic effects through regulation of multiple signaling cascades (abatement of phospho-p38, etc.) in hepatocellular carcinoma cells ^[4].
Isorhynchophylline (1-50 μM, 2 h) protects PC12 cells against Beta-Amyloid-induced apoptosis via PI3K/Akt signaling pathway^[5].
Isorhynchophylline (1-80 μM, 24 h) attenuates proliferation and migration of synovial fibroblasts via the FOXC1/β-catenin axis^[6].
Isorhynchophylline (5-200 μM, 48 h) alleviates cartilage degeneration in osteoarthritis by activating autophagy in chondrocytes^[7].
Isorhynchophylline (30 μM, 24 h) relieves ferroptosis-induced nerve damage after intracerebral hemorrhage via miR-122-5p/TP53/SLC7A11 pathway in hippocampal HT-22 cells^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

Rat vascular smooth muscle cells treated with Ang II (1.0 μM)
Concentration:

0.1, 1, 10 μM
Incubation Time:

24 h
Result:

Attenuated Ang II-induced cell proliferation in a concentration-dependent manner.
Abolished Ang-II induced VMSC proliferation at the highest concentration (10 μM).

Cell Cycle Analysis^[3]

Cell Line:

Rat vascular smooth muscle cells treated with Ang II (1.0 μM)
Concentration:

0.1, 1, 10 μM
Incubation Time:

24 h
Result:

Significantly decreased the percentage of cells in S and G2/M phases, and increased the percentage of cells in G0/G1 phase (1.0 μM and 10.0 μM).

Real Time qPCR^[3]

Cell Line:

Rat vascular smooth muscle cells treated with Ang II (1.0 μM)
Concentration:

0.1, 1, 10 μM
Incubation Time:

24 h
Result:

Suppressed the overexpression of c-fos mRNA in a concentration-dependent manner.
Decreased the over-expression of OPN and PCNA mRNA at 1.0 μM and 10.0 μM.

In Vivo

Isorhynchophylline (20-40 mg/kg, i.g., daily, 8 weeks) ameliorates D-galactose (HY-N0210)-induced learning and memory deficits in mice, at least in part, by enhancing the antioxidant status and anti-inflammatory effects of brain tissue through inhibition of NFκB signaling^[9].
Isorhynchophylline (50 μM, the first day of every week from the 5th to the 8th week after surgery) alleviates osteoarthritis in rat osteoarthritis models, with decreasing OARSI score and the reduction of joint space^[7].
Isorhynchophylline (10-40 mg/kg, i.g., 7 days) exerts antidepressant-like effects in mice at least in part by inhibiting monoamine oxidase^[10].
Isorhynchophylline (20-40 mg/kg, i.g., daily, 8 weeks) alleviates learning and memory impairments induced by aluminum chloride in mice^[11].
Isorhynchophylline (20 mg/kg, i.p., 14 or 42 days) alleviates silicon-dioxide-induced lung injury in mice, with reducing inflammatory responses and fibrosis^[12].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

D-galactose-induced cognitive deficits (Ten-week-old male ICR mice received a daily subcutaneous injection of D-gal at a dose of 100 mg/kg for 8 weeks)^[9]
Dosage:

20, 40 mg/kg
Administration:

Given intragastrically (i.g.), daily, 8 weeks
Result:

Shortened the mean latency to find the platform at the 4th day (20 mg/kg).
Led to a significant increase in the number of target crossings (40 mg/kg).
Attenuated oxidative stress (enhanced the level of GSH, attenuated MDA).
Decreased the production of NO and the mRNA expression of iNOS in the brain (40 mg/kg).
Decreased the production of PGE₂ and the mRNA expression of COX-2 (40 mg/kg).
Suppressed the protein level of p-IκBα (40 mg/kg).

Chemical Information

CAS. Nr. 6859-01-4
Appearance Solid
Molecular Weight 384.47
Formel C₂₂H₂₈N₂O₄
Color White to off-white
SMILES

O=C(NC1=C2C=CC=C1)[C@@]32[C@@](C[C@H](/C(C(OC)=O)=C\OC)[C@@H](CC)C4)([H])N4CC3
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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Journal Impact Factor

Int J Biol Macromol

Isorhynchophylline-loaded silk sericin/gelatin methacrylate crosslinked hydrogel: A synergistic platform for accelerated wound healing. [Abstract]

2025 Dec;334(Pt 2):148943. PMID: 41224043
Sci Rep

Exploring the molecular mechanisms of subarachnoid hemorrhage and potential therapeutic targets: insights from bioinformatics and drug prediction. [Abstract]

2025 Apr 15;15(1):12861. PMID: 40229542

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (260.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6010 mL

13.0049 mL

26.0098 mL

5 mM

0.5202 mL

2.6010 mL

5.2020 mL

10 mM

0.2601 mL

1.3005 mL

2.6010 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang K, et al. Macrophyllionium and macrophyllines A and B, oxindole alkaloids from Uncaria macrophylla. J Nat Prod. 2011 Jan 28;74(1):12-5. [Content Brief]

[2]. Yuan D, et al. Alkaloids from the leaves of Uncaria rhynchophylla and their inhibitory activity on NO production in lipopolysaccharide-activated microglia. J Nat Prod. 2008 Jul;71(7):1271-4. [Content Brief]

[3]. Zhang F, et al. Effects of isorhynchophylline on angiotensin II-induced proliferation in rat vascular smooth muscle cells. J Pharm Pharmacol. 2008 Dec;60(12):1673-8. [Content Brief]

[4]. Lee H, et al. Isorhynchophylline, a Potent Plant Alkaloid, Induces Apoptotic and Anti-Metastatic Effects in Human Hepatocellular Carcinoma Cells through the Modulation of Diverse Cell Signaling Cascades. Int J Mol Sci. 2017 May 19;18(5):1095. [Content Brief]

[5]. Xian YF, et al. Isorhynchophylline Protects PC12 Cells Against Beta-Amyloid-Induced Apoptosis via PI3K/Akt Signaling Pathway. Evid Based Complement Alternat Med. 2013;2013:163057. [Content Brief]

[6]. Wu Y, et al. Isorhynchophylline attenuates proliferation and migration of synovial fibroblasts via the FOXC1/β-catenin axis. Autoimmunity. 2023 Dec;56(1):2289868. [Content Brief]

[7]. Jiang J, et al. Isorhynchophylline alleviates cartilage degeneration in osteoarthritis by activating autophagy of chondrocytes. J Orthop Surg Res. 2023 Mar 2;18(1):154. [Content Brief]

[8]. Zhao H, et al. Isorhynchophylline Relieves Ferroptosis-Induced Nerve Damage after Intracerebral Hemorrhage Via miR-122-5p/TP53/SLC7A11 Pathway. Neurochem Res. 2021 Aug;46(8):1981-1994. [Content Brief]

[9]. Xian YF, et al. Isorhynchophylline improves learning and memory impairments induced by D-galactose in mice. Neurochem Int. 2014 Oct;76:42-9. [Content Brief]

[10]. Xian YF, et al. Antidepressant-Like Effect of Isorhynchophylline in Mice. Neurochem Res. 2017 Feb;42(2):678-685. [Content Brief]

[11]. Li HQ, et al. Isorhynchophylline alleviates learning and memory impairments induced by aluminum chloride in mice. Chin Med. 2018 Jun 14;13:29. [Content Brief]

[12]. Qiu M, et al. Protective effects of isorhynchophylline against silicon-dioxide-induced lung injury in mice. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):1125-1134. [Content Brief]

[13]. Guo H, et al. Isorhynchophylline protects against pulmonary arterial hypertension and suppresses PASMCs proliferation. Biochem Biophys Res Commun. 2014 Jul 18;450(1):729-34. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6010 mL	13.0049 mL	26.0098 mL	65.0246 mL
	5 mM	0.5202 mL	2.6010 mL	5.2020 mL	13.0049 mL
	10 mM	0.2601 mL	1.3005 mL	2.6010 mL	6.5025 mL
	15 mM	0.1734 mL	0.8670 mL	1.7340 mL	4.3350 mL
	20 mM	0.1300 mL	0.6502 mL	1.3005 mL	3.2512 mL
	25 mM	0.1040 mL	0.5202 mL	1.0404 mL	2.6010 mL
	30 mM	0.0867 mL	0.4335 mL	0.8670 mL	2.1675 mL
	40 mM	0.0650 mL	0.3251 mL	0.6502 mL	1.6256 mL
	50 mM	0.0520 mL	0.2601 mL	0.5202 mL	1.3005 mL
	60 mM	0.0433 mL	0.2167 mL	0.4335 mL	1.0837 mL
	80 mM	0.0325 mL	0.1626 mL	0.3251 mL	0.8128 mL
	100 mM	0.0260 mL	0.1300 mL	0.2601 mL	0.6502 mL

Keywords

Isorhynchophylline

2 Cited Publications

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Isorhynchophylline

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Publications Citing Use of MedChemExpress (MCE) Isorhynchophylline

Alle NF-κB Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Isorhynchophylline Related Classifications

Art. -Nr.: HY-N0766