Art. -Nr.: HY-B1473

Serotonin hydrochloride

(Synonyms: 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride)

41 Cited Publications

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Based on 41 publication(s) in Google Scholar

Serotonin (5-Hydroxytryptamine) hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist, with blood-brain barrier permeability. Serotonin hydrochloride is also a catechol O-methyltransferase (COMT) inhibitor with a K_i of 44 μM.

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Reinheit: 99.97%
CAS. Nr.: 153-98-0
Formel: C₁₀H₁₃ClN₂O
Molecular Weight:212.68

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Serotonin hydrochloride Chemical Structure

CAS. Nr.: 153-98-0

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (41)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Human Endogenous Metabolite

Microbial Metabolite

serotonin

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen :

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

Serotonin hydrochloride is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin hydrochloride also inhibits catechol O-methyltransferase (COMT), an enzyme that contributes to modultion the perception of pain, via non-competitive binding to the site bound by catechol substrates with a binding affinity comparable to the binding affinity of catechol itself (K_i= 44 μM). Results show that addition of 100 μM of Serotonin hydrochloride decreases the reaction velocity of COMT^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Serotonin hydrochloride produces robust hypersensitivity compare to saline-treated controls (p<0.001)^[1].
Due to the chemical structure of serotonin hydrochloride may prevent it from crossing the blood-brain barrier, but serotonin hydrochloride may utilize serotonin transport proteins to cross the endothelial cells of the blood-brain barrier^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 153-98-0
Appearance Solid
Molecular Weight 212.68
Formel C₁₀H₁₃ClN₂O
Color Off-white to light brown
SMILES

OC1=CC2=C(NC=C2CCN)C=C1.[H]Cl
Synonyms

5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (41)

All Cell Proliferation/Viability AssayELISACell Migration/Invasion AssayRT-PCRWB

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Cell Metab

Microbial phosphoketolase promotes histone lactylation to improve anti-TNF therapy efficacy in inflammatory bowel disease. [Abstract]

2026 May 13:S1550-4131(26)00150-6. PMID: 42134325
Immunity

Spatiotemporal dynamics of CXCL10 encode contextual immune information revealed by the genetically encoded fluorescent sensor. [Abstract]

2025 Sep 9;58(9):2320-2335.e9. PMID: 40818452
Cell Res

Light sensing enhances thermotolerance and competitive fitness via serotonergic signaling in an eyeless organism. [Abstract]

2026 Apr;36(4):286-299. PMID: 41764323
Cell Stem Cell

Vagal pathway activation links chronic stress to decline in intestinal stem cell function. [Abstract]

2025 Mar 20:S1934-5909(25)00084-0. PMID: 40120585
Nat Commun

Gut microbiota modulates aversive learning and memory of honeybees (Apis mellifera). [Abstract]

2025 Dec 18;16(1):11196. PMID: 41413037
J Exp Clin Cancer Res

Mechanisms of neural infiltration-mediated tumor metabolic reprogramming impacting immunotherapy efficacy in non-small cell lung cancer. [Abstract]

2024 Oct 10;43(1):284. PMID: 39385213
Small

Acupuncture Mechanics, Stimulation, and Cellular Serotonin Detection by a Magneto-Responsive Nanomesh Sensor. [Abstract]

2026 Mar;22(14):e11914. PMID: 41537659
Cell Rep Med

5-HT reuptake blockade induces pyroptosis in BRAFV600E-mutated melanomas via remodeling histone serotonylation. [Abstract]

2026 Jan 20;7(1):102537. PMID: 41494533
J Immunother Cancer

GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer. [Abstract]

2021 Jul;9(7):e002383. PMID: 34281987

Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]

Relative IDO1 transcription levels detected by quantitative reverse transcription PCR (qRT-PCR) analysis in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-HTP (500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.

Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2021 Jul;9(7):e002383. [Abstract]

Relative IDO1 levels detected by western blot in BT549 cells treated with dimethyl sulfoxide (DMSO), tetrahydrobiopterin (BH4, 10 µM), 5-HTP (500 µM), serotonin (10 µM), NAS (100 µM), and melatonin (1 µM) for 24 hours.
Acta Biomater

Sequential dual-drug delivery biomimetic hydrogel for temporal orchestration of acute neuroprotection and chronic regeneration in spinal cord injury. [Abstract]

2026 Mar:213:214-232. PMID: 41654155
Int J Biol Macromol

Aryl hydrocarbon receptor alleviates intestinal ischemia/reperfusion-induced epithelial barrier damage by inhibiting the 5-hydroxytryptamine-mediated complement C3 cascade. [Abstract]

2026 Jun:369:152765. PMID: 42214532
Food Res Int

Polysaccharide from Aloe vera gel improves intestinal stem cells dysfunction to alleviate intestinal barrier damage via 5-HT. [Abstract]

2025 Aug:214:116675. PMID: 40467243
Curr Res Food Sci

Tryptophan metabolic pathway plays a key role in the stress-induced emotional eating. [Abstract]

2024 Apr 26:8:100754. PMID: 38736909

Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: Curr Res Food Sci. 2024 Apr 26:8:100754. [Abstract]

Dose-response and time course of cell viability after in vitro administration of 5-HT(Serotonin);
J Pineal Res

Circadian-Related Serotonin/Melatonin Level Modulates Cisplatin Ototoxicity Susceptibility Depended on NOS3-NO Pathway. [Abstract]

2026 Jul;78(4):e70154. PMID: 42262169
Neurosci Bull

Targeting 5-HT to Alleviate Dose-Limiting Neurotoxicity in Nab-Paclitaxel-Based Chemotherapy. [Abstract]

2025 Jul;41(7):1229-1245. PMID: 40369268
Mar Drugs

GV-971 Ameliorates Chronic Restraint Stress-Induced Depression-like Phenotypes Accompanied by Reshaping of the Microbiota-Gut-Brain Axis. [Abstract]

2026 Jun;24(6):189. PMID: 42346775
Eur J Pharmacol

Establishment of a novel model of depression caused by olfactory dysfunction through intranasal administration of 3-methylindole. [Abstract]

2026 Jul 10:1029:178996. PMID: 42167669
iScience

5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation. [Abstract]

2025 Nov 19;28(12):114120. PMID: 41438086

Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2025 Nov 19;28(12):114120. [Abstract]

Scratch tests were used to observe the migration ability of MLE-12 cells treated with 5-HT (Serotonin, 10 μM) for 24 h.
iScience

Deficiency of Rab26 causes behavioral defects in mice through impaired trafficking of serotonin (5-HT) transporter. [Abstract]

2025 Jun 18;28(7):112931. PMID: 40687824

Serotonin hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2025 Jun 18;28(7):112931. [Abstract]

SH-SY5Y cells were treated with 10 μM Serotonin (5-HT), then the intracellular 5-HT was detected through ELISA assay for 0, 60, 120 min.
J Food Meas Charact

2026 Jan 30.
J Dent Sci

Serotonin promotes lip sensory recovery after inferior alveolar nerve transection via histone serotonylation. [Abstract]

2025 Oct;20(4):2363-2374. PMID: 41040553
Fitoterapia

The stem-derived bioactive fraction of Syringa oblata Lindl. Ameliorates insomnia via cAMP signaling-mediated regulation of neurotransmission, oxidative stress, and inflammation: A multi-omics study. [Abstract]

2026 Apr:190:107182. PMID: 41861857
Behav Brain Res

Serotonin mitigates depression in a rotenone-induced mouse Parkinson's disease model by inhibiting hippocampal neuronal pyroptosis and neuroinflammation. [Abstract]

2025 May 7:115620. PMID: 40345554
Biochem Biophys Res Commun

Glucose transports in the ileum: mechanism, regulation and physiological role of ileal glucose absorption. [Abstract]

2025 Sep 19:785:152676. PMID: 41005286
Indian J Ophthalmol

Ranitidine protects Müller cells against ferroptosis in diabetic retinopathy by regulating the AKT1/GSK3β pathway. [Abstract]

2026 Feb 1;74(2):250-258. PMID: 41581041
SSRN

2026 Jun 16.
bioRxiv

2026 May 29.
SSRN

2026 Apr 13.
SSRN

2026 Mar 6.
bioRxiv

2026 Mar 30.
bioRxiv

Quinone reductase 2 reads H3 serotonylation to support neuronal maturation. [Abstract]

2026 Mar 19:2026.03.17.712426. PMID: 41890023
bioRxiv

2025 Oct 14.
bioRxiv

2025 Sep 28.
SSRN

2025 Apr 24.
bioRxiv

2024 November 03.
bioRxiv

2024 November 03.
Res Sq

2024 Jun 11.
Research Square Preprint

2023 Dec 28.
bioRxiv

2023 Oct 13.
Authorea

September 19, 2022.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (470.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (470.19 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.7019 mL

23.5095 mL

47.0190 mL

5 mM

0.9404 mL

4.7019 mL

9.4038 mL

10 mM

0.4702 mL

2.3510 mL

4.7019 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protokoll

Cell Assay^[2]

For macrophage culture, peritoneal cavity cells are collected from WT and IL-13^-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×10⁶ cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin hydrochloride (10^-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13^-/- mice are injected subcutaneously with 100 mg/kg of Serotonin hydrochloride (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13^-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25. [Content Brief]

[2]. Mück-Šeler D, et al. Serotonin[J]. Periodicum biologorum, 2011, 113(1): 29-41.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	4.7019 mL	23.5095 mL	47.0190 mL	117.5475 mL
	5 mM	0.9404 mL	4.7019 mL	9.4038 mL	23.5095 mL
	10 mM	0.4702 mL	2.3509 mL	4.7019 mL	11.7547 mL
	15 mM	0.3135 mL	1.5673 mL	3.1346 mL	7.8365 mL
	20 mM	0.2351 mL	1.1755 mL	2.3509 mL	5.8774 mL
	25 mM	0.1881 mL	0.9404 mL	1.8808 mL	4.7019 mL
	30 mM	0.1567 mL	0.7836 mL	1.5673 mL	3.9182 mL
	40 mM	0.1175 mL	0.5877 mL	1.1755 mL	2.9387 mL
	50 mM	0.0940 mL	0.4702 mL	0.9404 mL	2.3509 mL
	60 mM	0.0784 mL	0.3918 mL	0.7836 mL	1.9591 mL
	80 mM	0.0588 mL	0.2939 mL	0.5877 mL	1.4693 mL
	100 mM	0.0470 mL	0.2351 mL	0.4702 mL	1.1755 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Serotonin hydrochloride

41 Cited Publications

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Serotonin hydrochloride

Publications Citing Use of MedChemExpress (MCE) Serotonin hydrochloride

Alle 5-HT Receptor Isoform-spezifische Produkte anzeigen

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (41)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Serotonin hydrochloride Related Classifications

HY-B1473 Related Classifications