ATP ditromethamine

ATP ditromethamine purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11. [Abstract]

Customer Validation
Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1.

ATP ditromethamine purchased from MedChemExpress. Usage Cited in: Transl Res. 2023 May:255:1-13. [Abstract]

Customer Validation
LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

ATP ditromethamine purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450. [Abstract]

Customer Validation
Primary BMDM cells were isolated from WT and Trim21^-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

ATP ditromethamine purchased from MedChemExpress. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356. [Abstract]

Customer Validation
Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

ATP ditromethamine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.  [Abstract]

Customer Validation
KCs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

ATP ditromethamine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.  [Abstract]

Customer Validation
KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.