dTAG-Fluorescein

dTAG-Fluorescein (dTAG-13) (30 minutes; 1 hour) potently engages purified CRBN-DDB1 protein in a ligand-displacement AlphaScreen assay^[5].
dTAG-Fluorescein (dTAG-13) effectively induces heterodimerization of purified CRBN-DDB1 and FKBP12^F36V proteins in a dimerization AlphaScreen assay^[5].
dTAG-Fluorescein (dTAG-13) (100 nM; 1-24 h) potently and selectively induces CRBN-dependent degradation of FKBP12^F36V-Nluc fusion protein in 293FTWT cells, with activity detectable at 100 nM within 1 hour of treatment^[5].
dTAG-Fluorescein (dTAG-13) (1 h) rapidly induces degradation of BRD4(short)-FKBP12^F36V fusion protein in MV4;11 cells without affecting endogenous FKBP12^WT[5].
dTAG-Fluorescein (dTAG-13) selectively induces degradation of endogenously tagged BRD4-FKBP12^F36V fusion protein in 293T knock-in cells without affecting BRD2, BRD3, or endogenous FKBP12^WT[5].
dTAG-Fluorescein (dTAG-13) (50 nM; 1-8 h) potently induces degradation of diverse nuclear and cytoplasmic FKBP12^F36V fusion proteins (including KRAS^G12V, EZH2, HDAC1, MYC, and PLK1) in MV4;11 cells, with activity detectable at 50 nM and varying degradation kinetics across targets^[5].
dTAG-Fluorescein (dTAG-13) (1-4 h) selectively depletes FKBP12_F36V-KRAS^G12V fusion protein in NIH/3T3 cells, achieving 58.3% depletion within 1 hour and 88.6% depletion within 4 hours, as measured by quantitative proteomics^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.