2. Membrane Transporter/Ion Channel
  3. Potassium Channel
  4. Dalazatide TFA

Dalazatide TFA  (Synonyms: ShK-186 TFA)

Cat. No.: HY-P3507A Purity: 98.44%
COA Handling Instructions

Dalazatide (ShK-186) TFA is a specific Kv1.3 potassium channel peptide inhibitor. Dalazatide TFA can be used in the study of autoimmune diseases such as multiple sclerosis (MS), lupus erythematosus, psoriasis, rheumatoid arthritis, type 1 diabetes and inflammatory bowel disease.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Dalazatide TFA Chemical Structure

Dalazatide TFA Chemical Structure

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1 mg USD 550 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1500 In-stock
Estimated Time of Arrival: December 31
10 mg USD 2500 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Dalazatide (ShK-186) TFA is a specific Kv1.3 potassium channel peptide inhibitor. Dalazatide TFA can be used in the study of autoimmune diseases such as multiple sclerosis (MS), lupus erythematosus, psoriasis, rheumatoid arthritis, type 1 diabetes and inflammatory bowel disease[1][2][3].

IC50 & Target

Kv1.3[1]
In Vitro

Dalazatide (ShK-186) (0-1000 pM) TFA blocks the Kv1.3 current in the Ova-specific GFP+ effector memory T (Tem) cells in a dose-dependent manner with a Kd of 65 ± 5 pM[3].
Dalazatide (0-100 nM; 3 days) TFA inhibits CCR7− T cell proliferation in a dose-dependent manner[3].
Dalazatide (100 nM; 30 min) TFA immobilizes effector memory T (Tem) cells at inflammatory sites by suppressing calcium signaling and thereby preventing β1 integrin activation[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[3]

Cell Line: CCR7− T cell[3]
Concentration: 0-100 nM
Incubation Time: 3 days
Result: Inhibited cell proliferation with an IC50 of 180 ± 37 pM.
In Vivo

Dalazatide (ShK-186) (100 μg/kg; s.c.; once) TFA inhibits delayed-type hypersensitivity and suppresses the in vivo motility and activation of Tem cells in rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Lewis rats rats, delayed-type hypersensitivity (DTH) model[3]
Dosage: 100 μg/kg
Administration: Subcutaneous injection, once
Result: Reduced DTH at all time points compared to rats given saline injections. Suppressed the proliferation of the Tem cells.
Molecular Weight

4556.10

Appearance

Solid

Formula

C186H297F3N57PS7
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

DalazatideShK-186ShK186ShK 186Potassium ChannelKcsAmultiple sclerosis (MS)lupus erythematosuspsoriasisrheumatoid arthritistype 1 diabetesinflammatory bowel diseaseInhibitorinhibitorinhibit

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