Zevontabart
Based on 1 Customer Validation
Zevontabart (MYTX-011 Antibody; Q-397) is a pH-dependent anti-c-MET antibody. Zevontabart regulates the trafficking process of c-MET to reduce receptor recycling, and enhances its own endocytosis and accumulation in c-MET-expressing cells. Zevontabart induces cytotoxicity in solid tumor cells and exerts anti-tumor activity in a non-small cell lung cancer xenograft mouse model. Zevontabart can be used to synthesize ADC, such as: MYTX-011. Zevontabart can be used in studies related to non-small cell lung cancer, with its corresponding isotype control being Human IgG1 kappa, Isotype Control (HY-P99001).
For research use only. We do not sell to patients.
- Purity: 99.46%
- CAS No.: 3060218-90-5
- Molecular Weight:143.999 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG1 kappa
Human
Zevontabart (50 nM; 120 s association, 300 s dissociation) binds to recombinant human c-MET in a pH-dependent manner, with a faster dissociation rate at the endolysosomal pH of 5.4 compared to the neutral pH of 7.4[1].
Zevontabart (10 nM; 0.5-3 h) accumulates highly in lysosomes of NCI-H1975 c-MET+ non-small cell lung cancer (NSCLC) cells[1].
Zevontabart (25 nM; 30 min) shows low colocalization with Rab4+ rapidly recycling endosomes in EBC-1 c-MET+ cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NCI-H1975 (c-MET+ NSCLC) cells transfected with LAMP1-RFP (lysosomal marker)
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Concentration:10 nmol/L
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Incubation Time:30 min, 1 h, 3 h
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Result:Showed equivalent colocalization with LAMP1-RFP+ lysosomes to the parent mAb at all time points, but exhibited significantly higher fluorescence intensity within lysosomes relative to the parent mAb.
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Cell Line:EBC-1 (c-MET+ cancer) cells transfected with Rab4-mRuby (fast-recycling endosome marker)
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Concentration:25 nmol/L
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Incubation Time:30 min
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Result:Showed significantly lower colocalization with Rab4-mRuby+ fast-recycling endosomes relative to the parent mAb.
| Species | Dose | Route | T1/2 |
|---|---|---|---|
| Cynomolgus Monkey[1] | 2.7 mg/kg | i.v. | 158 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID mice (6-8-week-old female; non-small cell lung cancer xenograft model)[1]
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Dosage:0.5 mg/kg; 1 mg/kg; 2 mg/kg; 4 mg/kg; 6 mg/kg
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Administration:i.v.; single dose
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Result:Significantly reduced tumor size and more effectively slowed tumor growth.
HGFR/c-Met
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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IgG1-kappa
ELISA, FACS, Functional assay
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Immobilized HGFR Protein, Human (HEK293, HY-P78490) can bind Zevontabart. The ED50 for this effect is 88.01 ng/mL.
Chemical Information
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CAS No. 3060218-90-5
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Appearance Liquid
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Molecular Weight 143.999 kDa
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Color Colorless to light yellow
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SMILES
[Zevontabart]
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Synonyms
MYTX-011 Antibody; Q-397
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Zevontabart Related Classifications
HY-P992146 Related Classifications
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