2. Autophagy Apoptosis
  3. p62 Autophagy Apoptosis
  4. EAD1

EAD1 is a Chloroquine (HY-17589A) analog with anticancer activity. EAD1 blocks autophagy, leading to the intracellular accumulation of autophagosome-related proteins LC3-II and p62. EAD1 induces cancer cell apoptosis and inhibits cancer cell proliferation. EAD1 can be used in the research of lung cancer and pancreatic cancer.

For research use only. We do not sell to patients.

EAD1

EAD1 Chemical Structure

CAS No. : 1644388-26-0

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Description

EAD1 is a Chloroquine (HY-17589A) analog with anticancer activity. EAD1 blocks autophagy, leading to the intracellular accumulation of autophagosome-related proteins LC3-II and p62. EAD1 induces cancer cell apoptosis and inhibits cancer cell proliferation. EAD1 can be used in the research of lung cancer and pancreatic cancer[1].

Cellular Effect
Cell Line Type Value Description References
BXPC-3 IC50
5.8 μM
Compound: 3h, EAD1
Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method
Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method
[PMID: 25699157]
HCC827 IC50
7.6 μM
Compound: 3h, EAD1
Antiproliferative activity against human HCC827 cells after 72 hrs by SRB method
Antiproliferative activity against human HCC827 cells after 72 hrs by SRB method
[PMID: 25699157]
NCI-H460 IC50
11 μM
Compound: 3h, EAD1
Antiproliferative activity against human H460 cells after 72 hrs by SRB method
Antiproliferative activity against human H460 cells after 72 hrs by SRB method
[PMID: 25699157]
In Vitro

EAD1 (compound 3h) potently inhibits the proliferation of H460, HCC827 and BxPC3 cancer cells at 72 h, with IC50 values of 11 μM, 7.6 μM and 5.8 μM, respectively[1].
EAD1 (6.5-100 μM; 24 h) potently inhibits colony formation of H460 non-small cell lung cancer cells[1].
EAD1 (5-75 μM; 24 h) induces concentration-dependent apoptosis in H460 non-small cell lung cancer (NSCLC) cells[1].
EAD1 (25-50 μM; 6 h) enhances the expression of punctate LC3 in H3122 non-small cell lung cancer cells[1].
EAD1 (1-25 μM, 10 μM; 2-72 h, 24 h) induces concentration- and time-dependent accumulation of autophagy-related proteins LC3-II and p62 in H460 non-small cell lung cancer (NSCLC) cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EAD1 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: H460 human NSCLC cells
Concentration: 6.5, 13, 25, 50, 100 μM
Incubation Time: 24 h
Result: Inhibited colony formation of H460 non-small cell lung cancer cells.

Apoptosis Analysis[1]

Cell Line: H460 human NSCLC cells
Concentration: 5, 25, 50, 75 μM
Incubation Time: 24 h
Result: Induced apoptosis in H460 cells in a concentration-dependent manner.

Western Blot Analysis[1]

Cell Line: H460 human NSCLC cells
Concentration: 1, 2.5, 5, 10, 25 μM (24 h incubation); 10 μM (time-course incubation)
Incubation Time: 2, 4, 6, 24, 48, 72 h (10 μM concentration); 24 h (1-25 μM concentration)
Result: Caused concentration- and time-dependent increases in autophagosome-bound LC3-II levels.
Showed larger increase in LC3-II levels at each tested concentration compared to HCQ.
Made the effect of 25 μM HCQ on LC3-II approximately equal to that of 5 μM EAD1.
Caused concentration-dependent accumulation of p62 (an autophagy substrate), with more potent activity than HCQ.
Molecular Weight

484.42

Formula

C24H27Cl2N7
CAS No.
SMILES

CN(CCNCC1=CN(CC2=CC=C(Cl)C=C2)N=N1)CCNC3=CC=NC4=CC(Cl)=CC=C34
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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EAD1 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EAD11644388-26-0EAD 1EAD-1p62AutophagyApoptosisSequestosome-1SQSTM1antiproliferativelungpancreaticcancerInhibitorinhibitorinhibit

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