EAD1
EAD1 is a Chloroquine (HY-17589A) analog with anticancer activity. EAD1 blocks autophagy, leading to the intracellular accumulation of autophagosome-related proteins LC3-II and p62. EAD1 induces cancer cell apoptosis and inhibits cancer cell proliferation. EAD1 can be used in the research of lung cancer and pancreatic cancer.
For research use only. We do not sell to patients.
- CAS No.: 1644388-26-0
- Formula: C24H27Cl2N7
- Molecular Weight:484.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BXPC-3 | IC50 |
5.8 μM
Compound: 3h, EAD1
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Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method
Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method
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[PMID: 25699157] |
| HCC827 | IC50 |
7.6 μM
Compound: 3h, EAD1
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Antiproliferative activity against human HCC827 cells after 72 hrs by SRB method
Antiproliferative activity against human HCC827 cells after 72 hrs by SRB method
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[PMID: 25699157] |
| NCI-H460 | IC50 |
11 μM
Compound: 3h, EAD1
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Antiproliferative activity against human H460 cells after 72 hrs by SRB method
Antiproliferative activity against human H460 cells after 72 hrs by SRB method
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[PMID: 25699157] |
EAD1 (compound 3h) potently inhibits the proliferation of H460, HCC827 and BxPC3 cancer cells at 72 h, with IC50 values of 11 μM, 7.6 μM and 5.8 μM, respectively[1].
EAD1 (6.5-100 μM; 24 h) potently inhibits colony formation of H460 non-small cell lung cancer cells[1].
EAD1 (5-75 μM; 24 h) induces concentration-dependent apoptosis in H460 non-small cell lung cancer (NSCLC) cells[1].
EAD1 (25-50 μM; 6 h) enhances the expression of punctate LC3 in H3122 non-small cell lung cancer cells[1].
EAD1 (1-25 μM, 10 μM; 2-72 h, 24 h) induces concentration- and time-dependent accumulation of autophagy-related proteins LC3-II and p62 in H460 non-small cell lung cancer (NSCLC) cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H460 human NSCLC cells
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Concentration:6.5, 13, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Inhibited colony formation of H460 non-small cell lung cancer cells.
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Cell Line:H460 human NSCLC cells
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Concentration:5, 25, 50, 75 μM
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Incubation Time:24 h
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Result:Induced apoptosis in H460 cells in a concentration-dependent manner.
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Cell Line:H460 human NSCLC cells
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Concentration:1, 2.5, 5, 10, 25 μM (24 h incubation); 10 μM (time-course incubation)
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Incubation Time:2, 4, 6, 24, 48, 72 h (10 μM concentration); 24 h (1-25 μM concentration)
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Result:Caused concentration- and time-dependent increases in autophagosome-bound LC3-II levels.
Showed larger increase in LC3-II levels at each tested concentration compared to HCQ.
Made the effect of 25 μM HCQ on LC3-II approximately equal to that of 5 μM EAD1.
Caused concentration-dependent accumulation of p62 (an autophagy substrate), with more potent activity than HCQ.
Chemical Information
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CAS No. 1644388-26-0
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Molecular Weight 484.42
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Formula C24H27Cl2N7
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SMILES
CN(CCNCC1=CN(CC2=CC=C(Cl)C=C2)N=N1)CCNC3=CC=NC4=CC(Cl)=CC=C34
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
EAD1 Related Classifications
HY-123056 Related Classifications
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