1. Autophagy
  2. Autophagy
  3. Autophinib

Autophinib 

Cat. No.: HY-101920 Purity: 99.55%
Handling Instructions

Autophinib is a potent, selective autophagy inhibitor with IC50s of 90 nM and 40 nM for starvation- and Rapamycin-induced autophagy, respectively. Autophinib is also an ATP competitive Vacuolar Protein Sorting 34 (VPS34) inhibitor with an IC50 of 19 nM. Autophinib inhibits autophagy induced by starvation or Rapamycin by targeting VPS34.

For research use only. We do not sell to patients.

Autophinib Chemical Structure

Autophinib Chemical Structure

CAS No. : 1644443-47-9

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10 mM * 1  mL in DMSO USD 95 In-stock
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10 mg USD 120 In-stock
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50 mg USD 350 In-stock
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100 mg USD 550 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Autophinib purchased from MCE. Usage Cited in: Cancer Sci. 2020 May; 111(5): 1542-1554.

    Western blotting shows that the decreased protein expression of P62 in Per1‐OE SCC15 cells is remarkably increased after the addition of Autophinib, while the increased LC3BII/LC3BI ratio and Beclin1 expression are remarkably decreased.
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    Description

    Autophinib is a potent, selective autophagy inhibitor with IC50s of 90 nM and 40 nM for starvation- and Rapamycin-induced autophagy, respectively. Autophinib is also an ATP competitive Vacuolar Protein Sorting 34 (VPS34) inhibitor with an IC50 of 19 nM. Autophinib inhibits autophagy induced by starvation or Rapamycin by targeting VPS34[1].

    IC50 & Target

    IC50: 19 nM (VPS34), 40 nM (Rapamycin-induced autophagy), 90 nM (starvation-induced autophagy)[1]

    In Vitro

    Autophinib (0.01-1 μM) inhibits LC3 lipidation to form LC3-II in a dose-dependent manner in starved MCF7-LC3 cells. Consistent with inhibition of autophagic flux, Autophinib also inhibits p62 degradation by autophagy dose-dependently in MCF7-LC3 cells[1].
    Autophinib enhances cell death (EC50 of 264 nM) of starved cells as compared to fed cells, which occurred via the induction of apoptosis (EC50 of 234 nM) in MCF7 cells[1].

    Molecular Weight

    346.73

    Formula

    C₁₄H₁₁ClN₆O₃

    CAS No.

    1644443-47-9

    SMILES

    CC1=CC(NC2=NC(OC3=CC=C([N+]([O-])=O)C=C3)=NC(Cl)=C2)=NN1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (86.52 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8841 mL 14.4204 mL 28.8409 mL
    5 mM 0.5768 mL 2.8841 mL 5.7682 mL
    10 mM 0.2884 mL 1.4420 mL 2.8841 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 0.5 mg/mL (1.44 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 0.5 mg/mL (1.44 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    AutophinibAutophagyInhibitorinhibitorinhibit

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    Product Name:
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    Cat. No.:
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