QX77
Based on 5 publication(s) in Google Scholar
QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 can impede self-renewal and promote differentiation of ES cells.
For research use only. We do not sell to patients.
- Purity: 99.48%
- CAS No.: 1798331-92-6
- Formula: C16H13ClN2O2
- Molecular Weight:300.74
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) QX77
More
Biological Activity
CMA[1]
QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/-?MEFs[1].? In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells[1].Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane[1].QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells[1].? QX77 protects cystinotic cells from the increased susceptibility to? tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression[1].QX77 (10 μM;? 0, 3 or 6 days) activates CMA and increases LAMP2A expression in? D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ES D3 cell line; E14TG2a (E14) cell line
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Concentration:10 μM
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Incubation Time:3 or 6 days
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Result:Increased LAMP2A expression and decreased SOX2 and Oct4 protein expression.
Chemical Information
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CAS No. 1798331-92-6
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Appearance Solid
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Molecular Weight 300.74
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Formula C16H13ClN2O2
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Color Light yellow to yellow
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SMILES
CC(NC1=CC=C(C2=NC3=CC=C(Cl)C=C3OC2)C=C1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Metab
Chaperone-mediated autophagy sustains muscle stem cell regenerative functions but declines with age. [Abstract]2025 Dec;7(12):2571-2588. PMID: 41339968 -
Autophagy
Chaperone-mediated autophagy degrades SERPINA1E342K/α1-antitrypsin Z variant and alleviates cell stress. [Abstract]2025 Mar 20. PMID: 40114294 -
Adv Sci (Weinh)
HSPA8 Activates Wnt/β-Catenin Signaling to Facilitate BRAF V600E Colorectal Cancer Progression by CMA-Mediated CAV1 Degradation. [Abstract]2024 Jan;11(3):e2306535. PMID: 37973552 -
Sci Adv
Deficient chaperone-mediated autophagy facilitates LPS-induced microglial activation via regulation of the p300/NF-κB/NLRP3 pathway. [Abstract]2023 Oct 6;9(40):eadi8343. PMID: 37801503 -
J Neuroinflammation
p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease. [Abstract]2021 Dec 20;18(1):295. PMID: 34930303
Solvent & Solubility
DMSO : 15 mg/mL (49.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (33.25 mM); Suspended solution; Need ultrasonic
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Jinzhong Zhang, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017 Jun 23;292(25):10328-10346 [Content Brief]
[2]. Yi Xu, et al. Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells. Science. 2020 Jul 24;369(6502):397-403. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3251 mL | 16.6257 mL | 33.2513 mL | 83.1283 mL |
| 5 mM | 0.6650 mL | 3.3251 mL | 6.6503 mL | 16.6257 mL | |
| 10 mM | 0.3325 mL | 1.6626 mL | 3.3251 mL | 8.3128 mL | |
| 15 mM | 0.2217 mL | 1.1084 mL | 2.2168 mL | 5.5419 mL | |
| 20 mM | 0.1663 mL | 0.8313 mL | 1.6626 mL | 4.1564 mL | |
| 25 mM | 0.1330 mL | 0.6650 mL | 1.3301 mL | 3.3251 mL | |
| 30 mM | 0.1108 mL | 0.5542 mL | 1.1084 mL | 2.7709 mL | |
| 40 mM | 0.0831 mL | 0.4156 mL | 0.8313 mL | 2.0782 mL |
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