EAD1 (trihydrochloride)
Based on 1 Customer Validation
EAD1 trihydrochloride is a Chloroquine (HY-17589A) analog with anticancer activity. EAD1 trihydrochloride blocks autophagy, leading to the intracellular accumulation of autophagosome-related proteins LC3-II and p62. EAD1 trihydrochloride induces cancer cell apoptosis and inhibits cancer cell proliferation. EAD1 trihydrochloride can be used in the research of lung cancer and pancreatic cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C24H30Cl5N7
- 分子量:593.81
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
EAD1 (compound 3h) (72 h) trihydrochloride potently inhibits the proliferation of H460, HCC827 and BxPC3 cancer cells at 72 h, with IC50 values of 11 μM, 7.6 μM and 5.8 μM, respectively[1].
EAD1 (6.5-100 μM; 24 h) trihydrochloride potently inhibits colony formation of H460 non-small cell lung cancer cells[1].
EAD1 (5-75 μM; 24 h) trihydrochloride induces concentration-dependent apoptosis in H460 non-small cell lung cancer (NSCLC) cells[1].
EAD1 (25-50 μM; 6 h) trihydrochloride enhances the expression of punctate LC3 in H3122 non-small cell lung cancer cells[1].
EAD1 (1-25 μM, 10 μM; 2-72 h, 24 h) trihydrochloride induces concentration- and time-dependent accumulation of autophagy-related proteins LC3-II and p62 in H460 non-small cell lung cancer (NSCLC) cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Autophagy
- Apoptosis Solutions
- Lysosome and acidic-vesicle live-cell staining
- MTT Cell Proliferation Assay
- Macroautophagy Solutions
化学情報
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性状 Solid
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分子量 593.81
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分子式 C24H30Cl5N7
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SMILES
CN(CCNC1=CC=NC2=CC(Cl)=CC=C12)CCNCC3=CN(N=N3)CC4=CC=C(C=C4)Cl.Cl.Cl.Cl
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
EAD1 (trihydrochloride) 関連分類
HY-123056B 関連分類
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