2. NF-κB TGF-beta/Smad Stem Cell/Wnt Protein Tyrosine Kinase/RTK
  3. NF-κB TGF-beta/Smad Syk
  4. Flavanomarein

Flavanomarein is a substance with cytoprotective, anti-inflammatory and antioxidant activities, with a Ka of 3.064e-5 M against human Syk. Flavanomarein enhances the phosphorylation level of AKT, regulates the expression of PKC-δ, P85α, PKC-β1, Sirt1, Bcl-2 and ICAD, and inhibits the nuclear translocation of NF-κB p65. Flavanomarein regulates EMT marker proteins, promotes the proliferation of HK-2 cells, and protects neuronal cells from 6-OHDA-induced neurotoxic damage. Flavanomarein can be used in studies related to Parkinson's disease and diabetic nephropathy.

For research use only. We do not sell to patients.

Flavanomarein

Flavanomarein Chemical Structure

CAS No. : 577-38-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

Other Forms of Flavanomarein:

Flavanomarein Related Antibodies

Top Publications Citing Use of Products

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Flavanomarein is a substance with cytoprotective, anti-inflammatory and antioxidant activities, with a Ka of 3.064e-5 M against human Syk. Flavanomarein enhances the phosphorylation level of AKT, regulates the expression of PKC-δ, P85α, PKC-β1, Sirt1, Bcl-2 and ICAD, and inhibits the nuclear translocation of NF-κB p65. Flavanomarein regulates EMT marker proteins, promotes the proliferation of HK-2 cells, and protects neuronal cells from 6-OHDA-induced neurotoxic damage. Flavanomarein can be used in studies related to Parkinson's disease and diabetic nephropathy.[1][2]

In Vitro

Flavanomarein (1.25-100 μM; 24 h preincubation followed by 24 h 6-OHDA treatment) protects PC12 cells from 6-OHDA-induced neurotoxicity by upregulating the PI3K/AKT signaling pathway, attenuating the NF-κB signaling pathway, preserving mitochondrial function, and inhibiting apoptosis, with significant effects observed at concentrations of 1.25, 25, 50, and 100 μM after 24 h preincubation[1].
Flavanomarein (1.25-100 μM; 24 h preincubation followed by 6 h 6-OHDA treatment) protects primary rat cortical neurons from 6-OHDA-induced apoptosis and cytotoxicity, with significant effects observed at concentrations of 1.25, 25, 50, and 100 μM after 24 h preincubation followed by 6 h 6-OHDA treatment[1].
Flavanomarein (200-400 μM; 24 h; 100-400 μM; 48 h) significantly promotes the proliferation of normal glucose-cultured HK-2 cells[2].
Flavanomarein (1-200 μM; 48 h) dose-dependently increases the viability of HG-stimulated HK-2 cells, reversing HG-induced cell viability reduction[2].
Flavanomarein (100 μM; 48 h) inhibits HG-induced EMT morphological changes in HK-2 cells[2].
Flavanomarein (1-80 μM; 48 h) dose-dependently enhances E-cadherin expression and reduces α-SMA, Vimentin, and FN expression in HG-stimulated HK-2 cells, exerting an anti-EMT effect[2].
Flavanomarein directly binds to Syk with high affinity, as indicated by a CDOCKER interaction energy of -75.1069 kcal/mol and specific hydrogen bonding with key Syk residues[2].
Flavanomarein (6.25-200 μM; real-time binding detection) directly binds to Syk in a concentration-dependent manner with an equilibrium dissociation constant of 3.064 × 10-5 M, confirming Syk as a direct target[2].
Flavanomarein (80 μM; 48 h) enhances E-cadherin expression and reduces α-SMA, Vimentin, and FN expression in HG-stimulated HK-2 cells, with synergistic effects when combined with a Syk inhibitor[2].
Flavanomarein (80 μM; 48 h) inhibits activation of the Syk/TGF-β1/Smad signaling pathway in HG-stimulated HK-2 cells, reducing levels of p-Syk, p-Smad2, TGF-β1, and Smad4[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Flavanomarein Related Antibodies

Cell Proliferation Assay[2]

Cell Line: human proximal tubular epithelial HK-2 cells (normal glucose conditions)
Concentration: 200-400 μM (24 h); 100-400 μM (48 h)
Incubation Time: 24 h (200-400 μM); 48 h (100-400 μM)
Result: Significantly enhanced HK-2 cell viability relative to control, indicating induction of cell proliferation.

Cell Viability Assay[2]

Cell Line: high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells
Concentration: 1-200 μM
Incubation Time: 48 h
Result: Induced a dose-dependent increase in cell viability relative to both control and HG-only groups; reversed the decrease where HG-treated cells showed ~80% viability of control.

Western Blot Analysis[2]

Cell Line: high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells
Concentration: 1-80 μM
Incubation Time: 48 h
Result: Induced a concentration-dependent increase in E-cadherin protein levels relative to the HG-only group; caused a dose-dependent decrease in α-SMA, Vimentin, and FN protein levels relative to the HG-only group.

Western Blot Analysis[2]

Cell Line: high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells (co-treatment with Syk inhibitor)
Concentration: 80 μM
Incubation Time: 48 h
Result: Markedly increased E-cadherin protein levels and significantly decreased α-SMA, Vimentin, and FN protein levels relative to the HG-only group; co-treatment with a Syk inhibitor resulted in the highest E-cadherin increase.

Western Blot Analysis[2]

Cell Line: high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells
Concentration: 80 μM
Incubation Time: 48 h
Result: Significantly decreased the relative expression of p-Syk/Syk, p-Smad2/Smad2, TGF-β1, and Smad4 protein levels relative to the HG-only group.
Molecular Weight

450.39

Formula

C21H22O11
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C[C@@H](C2=CC=C(O)C(O)=C2)OC3=C(O)C(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)=CC=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (74.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2203 mL 11.1015 mL 22.2030 mL
5 mM 0.4441 mL 2.2203 mL 4.4406 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2203 mL 11.1015 mL 22.2030 mL 55.5074 mL
5 mM 0.4441 mL 2.2203 mL 4.4406 mL 11.1015 mL
10 mM 0.2220 mL 1.1101 mL 2.2203 mL 5.5507 mL
15 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7005 mL
20 mM 0.1110 mL 0.5551 mL 1.1101 mL 2.7754 mL
25 mM 0.0888 mL 0.4441 mL 0.8881 mL 2.2203 mL
30 mM 0.0740 mL 0.3700 mL 0.7401 mL 1.8502 mL
40 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL
50 mM 0.0444 mL 0.2220 mL 0.4441 mL 1.1101 mL
60 mM 0.0370 mL 0.1850 mL 0.3700 mL 0.9251 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Flavanomarein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flavanomarein577-38-8NF-κBTGF-beta/SmadSykNuclear factor-κBNuclear factor-kappaBTransforming growth factor betaSpleen tyrosine kinasePKC-δP85αHK-2 cellsAKTPKC-β1NF-κB p65Sirt1Bcl-2ICADInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Flavanomarein
Cat. No.:
HY-N7675
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.