Cat. No.: HY-N7675

Flavanomarein

Customer Review

Based on 1 Customer Validation

Flavanomarein is a substance with cytoprotective, anti-inflammatory and antioxidant activities, with a K_a of 3.064e-5 M against human Syk. Flavanomarein enhances the phosphorylation level of AKT, regulates the expression of PKC-δ, P85α, PKC-β1, Sirt1, Bcl-2 and ICAD, and inhibits the nuclear translocation of NF-κB p65. Flavanomarein regulates EMT marker proteins, promotes the proliferation of HK-2 cells, and protects neuronal cells from 6-OHDA-induced neurotoxic damage. Flavanomarein can be used in studies related to Parkinson's disease and diabetic nephropathy.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 577-38-8
Formula: C₂₁H₂₂O₁₁
Molecular Weight:450.39

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Flavanomarein Chemical Structure

CAS No.: 577-38-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All NF-κB Isoforms :

All TGF-beta/Smad Isoforms :

Smad1
Smad3

In Vitro

Flavanomarein (1.25-100 μM; 24 h preincubation followed by 24 h 6-OHDA treatment) protects PC12 cells from 6-OHDA-induced neurotoxicity by upregulating the PI3K/AKT signaling pathway, attenuating the NF-κB signaling pathway, preserving mitochondrial function, and inhibiting apoptosis, with significant effects observed at concentrations of 1.25, 25, 50, and 100 μM after 24 h preincubation^[1].
Flavanomarein (1.25-100 μM; 24 h preincubation followed by 6 h 6-OHDA treatment) protects primary rat cortical neurons from 6-OHDA-induced apoptosis and cytotoxicity, with significant effects observed at concentrations of 1.25, 25, 50, and 100 μM after 24 h preincubation followed by 6 h 6-OHDA treatment^[1].
Flavanomarein (200-400 μM; 24 h; 100-400 μM; 48 h) significantly promotes the proliferation of normal glucose-cultured HK-2 cells^[2].
Flavanomarein (1-200 μM; 48 h) dose-dependently increases the viability of HG-stimulated HK-2 cells, reversing HG-induced cell viability reduction^[2].
Flavanomarein (100 μM; 48 h) inhibits HG-induced EMT morphological changes in HK-2 cells^[2].
Flavanomarein (1-80 μM; 48 h) dose-dependently enhances E-cadherin expression and reduces α-SMA, Vimentin, and FN expression in HG-stimulated HK-2 cells, exerting an anti-EMT effect^[2].
Flavanomarein directly binds to Syk with high affinity, as indicated by a CDOCKER interaction energy of -75.1069 kcal/mol and specific hydrogen bonding with key Syk residues^[2].
Flavanomarein (6.25-200 μM; real-time binding detection) directly binds to Syk in a concentration-dependent manner with an equilibrium dissociation constant of 3.064 × 10^-5 M, confirming Syk as a direct target^[2].
Flavanomarein (80 μM; 48 h) enhances E-cadherin expression and reduces α-SMA, Vimentin, and FN expression in HG-stimulated HK-2 cells, with synergistic effects when combined with a Syk inhibitor^[2].
Flavanomarein (80 μM; 48 h) inhibits activation of the Syk/TGF-β1/Smad signaling pathway in HG-stimulated HK-2 cells, reducing levels of p-Syk, p-Smad2, TGF-β1, and Smad4^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

human proximal tubular epithelial HK-2 cells (normal glucose conditions)
Concentration:

200-400 μM (24 h); 100-400 μM (48 h)
Incubation Time:

24 h (200-400 μM); 48 h (100-400 μM)
Result:

Significantly enhanced HK-2 cell viability relative to control, indicating induction of cell proliferation.

Cell Viability Assay^[2]

Cell Line:

high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells
Concentration:

1-200 μM
Incubation Time:

48 h
Result:

Induced a dose-dependent increase in cell viability relative to both control and HG-only groups; reversed the decrease where HG-treated cells showed ~80% viability of control.

Western Blot Analysis^[2]

Cell Line:

high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells
Concentration:

1-80 μM
Incubation Time:

48 h
Result:

Induced a concentration-dependent increase in E-cadherin protein levels relative to the HG-only group; caused a dose-dependent decrease in α-SMA, Vimentin, and FN protein levels relative to the HG-only group.

Western Blot Analysis^[2]

Cell Line:

high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells (co-treatment with Syk inhibitor)
Concentration:

80 μM
Incubation Time:

48 h
Result:

Markedly increased E-cadherin protein levels and significantly decreased α-SMA, Vimentin, and FN protein levels relative to the HG-only group; co-treatment with a Syk inhibitor resulted in the highest E-cadherin increase.

Western Blot Analysis^[2]

Cell Line:

high glucose (HG)-stimulated human proximal tubular epithelial HK-2 cells
Concentration:

80 μM
Incubation Time:

48 h
Result:

Significantly decreased the relative expression of p-Syk/Syk, p-Smad2/Smad2, TGF-β1, and Smad4 protein levels relative to the HG-only group.

Chemical Information

CAS No. 577-38-8
Appearance Solid
Molecular Weight 450.39
Formula C₂₁H₂₂O₁₁
Color White to off-white
SMILES

O=C1C[C@@H](C2=CC=C(O)C(O)=C2)OC3=C(O)C(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)=CC=C13
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Polyphenols
Initial Source
- Plants
- Compositae
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (74.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2203 mL

11.1015 mL

22.2030 mL

5 mM

0.4441 mL

2.2203 mL

4.4406 mL

10 mM

0.2220 mL

1.1101 mL

2.2203 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Le L, et al. The protective effects of the native flavanone flavanomarein on neuronal cells damaged by 6-OHDA. Phytomedicine. 2019;53:193-204. [Content Brief]

[2]. Zhang NN, et al. Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase. Sci Rep. 2020;10(1):439. Published 2020 Jan 16. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2203 mL	11.1015 mL	22.2030 mL	55.5074 mL
	5 mM	0.4441 mL	2.2203 mL	4.4406 mL	11.1015 mL
	10 mM	0.2220 mL	1.1101 mL	2.2203 mL	5.5507 mL
	15 mM	0.1480 mL	0.7401 mL	1.4802 mL	3.7005 mL
	20 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7754 mL
	25 mM	0.0888 mL	0.4441 mL	0.8881 mL	2.2203 mL
	30 mM	0.0740 mL	0.3700 mL	0.7401 mL	1.8502 mL
	40 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL
	50 mM	0.0444 mL	0.2220 mL	0.4441 mL	1.1101 mL
	60 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9251 mL

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Flavanomarein

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

TGF-beta/Smad Compound Library

Wnt/Hedgehog/Notch Compound Library

Natural Product Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Anti-diabetic Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Phenols Library

Pyroptosis Compound Library

Flavonoids Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Plant-Sourced Natural Product Library

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Anti-Pulmonary Fibrosis Compound Library

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Cancer Stem Cells Compound Library

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Bioactive Compound Library Max

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

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Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

All NF-κB Isoforms

All TGF-beta/Smad Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Flavanomarein Related Classifications

Cat. No.: HY-N7675