Formononetin (Synonyms: Biochanin B; Flavosil; Formononetol)

Name: Formononetin
Brand: MedChemExpress
SKU: HY-N0183
Rating: 5.0 (24 reviews)

Cat. No.: HY-N0183 Purity: 99.21%: Data Sheet
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Formononetin is a potent FGFR2 inhibitor with an IC₅₀ of ~4.31 μM. Formononetin potently inhibits angiogenesis and tumor growth.

For research use only. We do not sell to patients.

CAS No. : 485-72-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 24 publication(s) in Google Scholar

Other Forms of Formononetin:

Formononetin-d₃-1 In-stock
Formononetin (Standard) In-stock

24 Publications Citing Use of MCE Formononetin

Histological Imaging/Staining

Cell Imaging/Staining

: Formononetin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun 9:145:156960. [Abstract]; Formononetin (25-100 mg/kg, i.g.) administration ameliorated Ethanol-Induced liver injury in 12 weeks aged mice. Representative images of H&E staining.

: Formononetin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun 9:145:156960. [Abstract]; Formononetin (25-100 mg/kg, i.g.) administration ameliorated Ethanol-Induced liver injury in 12 weeks aged mice. Representative images of TUNEL staining.

: Formononetin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun 9:145:156960. [Abstract]; Formononetin (25-100 mg/kg, i.g.) protected against Ethanol induced aldehyde toxicity in 12 weeks aged mice. Representative images of Dihydroethidium (DHE) staining and 4-Hydroxynonenal (4-HNE) staining.

: Formononetin purchased from MedChemExpress. Usage Cited in: Mol Med. 2019 Dec;20(6):4984-4992. [Abstract]; Formononetin (10-60 mg/kg, i.p., daily for 2 weeks) suppressed Monocrotaline (MCT)-induced pulmonary vascular remodeling in male Sprague-Dawley (7-weeks-old) rats.

: Formononetin purchased from MedChemExpress. Usage Cited in: Mol Med. 2019 Dec;20(6):4984-4992. [Abstract]; Formononetin (10-60 mg/kg, i.p., daily for 2 weeks) attenuated decreases in Bax and cleaved caspase-3 expression induced by Monocrotaline (MCT).

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Biological Activity
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Description

Formononetin is a potent FGFR2 inhibitor with an IC₅₀ of ~4.31 μM. Formononetin potently inhibits angiogenesis and tumor growth^[1].

IC₅₀ & Target^[1]

FGFR2

4.31 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	2.87 μg/mL Compound: 11	Cytotoxicity against human A549 cells after 7 days Cytotoxicity against human A549 cells after 7 days	[PMID: 8201311]
A549	IC₅₀	> 100 μM Compound: 13	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 18440233]
B16-BL6	IC₅₀	> 100 μM Compound: 13	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 18440233]
BV-2	IC₅₀	13.19 μM Compound: 2	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 28911817]
BV-2	IC₅₀	> 100 μM Compound: 19	Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 25466192]
BV-2	IC₅₀	> 50 μM Compound: 9	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production	[PMID: 29482940]
DU-145	IC₅₀	> 100 μM Compound: Formononetin	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 cells after 72 hrs by MTT assay	[PMID: 22633834]
HCT-116	GI₅₀	64 μM Compound: 12e	Antiproliferative activity against human HCT116 cell line Antiproliferative activity against human HCT116 cell line	[PMID: 16789754]
HCT-116	IC₅₀	60.97 μM Compound: Formononetin	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 22633834]
HT-1080	IC₅₀	> 100 μM Compound: 13	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 18440233]
HT-29	ED₅₀	6.43 μg/mL Compound: 11	Cytotoxicity against human HT-29 cells after 7 days Cytotoxicity against human HT-29 cells after 7 days	[PMID: 8201311]
HT-29	GI₅₀	74 μM Compound: 12e	Antiproliferative activity against human HT29 cell line Antiproliferative activity against human HT29 cell line	[PMID: 16789754]
HeLa	IC₅₀	8.51 μM Compound: Formononetin	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 22633834]
HeLa	IC₅₀	> 100 μM Compound: 13	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 18440233]
HepG2	IC₅₀	60.5 μM Compound: 15	Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24974349]
MCF7	ED₅₀	1.96 μg/mL Compound: 11	Cytotoxicity against human MCF7 cells after 7 days Cytotoxicity against human MCF7 cells after 7 days	[PMID: 8201311]
MCF7	GI₅₀	81.4 μM Compound: 12e	Antiproliferative activity against human MCF7 cell line Antiproliferative activity against human MCF7 cell line	[PMID: 16789754]
MDA-MB-468	GI₅₀	27.2 μM Compound: 12e	Antiproliferative activity against human MDA-MB-468 cell line Antiproliferative activity against human MDA-MB-468 cell line	[PMID: 16789754]
MDCK	CC₅₀	123 μM Compound: 3	Cytotoxicity against MDCK cells after 72 hrs Cytotoxicity against MDCK cells after 72 hrs	[PMID: 24313801]
MDCK	IC₅₀	38.2 μM Compound: 3	Antiviral activity against Influenza A virus J/8178/09 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs Antiviral activity against Influenza A virus J/8178/09 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs	[PMID: 24313801]
MDCK	IC₅₀	42.6 μM Compound: 3	Antiviral activity against Influenza A virus (A/Hong Kong/1/1968(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs Antiviral activity against Influenza A virus (A/Hong Kong/1/1968(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs	[PMID: 24313801]
MGC-803	IC₅₀	> 100 μM Compound: 1	Cytotoxicity against human MGC803 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human MGC803 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 28038329]
N9	IC₅₀	> 100 μM Compound: 4	Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 28073678]
PC-3	IC₅₀	57.01 μM Compound: 1	Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 28038329]
Panel NCI-60 (60 carcinoma cell lines)	GI₅₀	50 μM Compound: 12e	Antiproliferative activity against human NCI60 cell line Antiproliferative activity against human NCI60 cell line	[PMID: 16789754]
RAW264.7	IC₅₀	56.1 μM Compound: 6	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23743282]
SGC-7901	IC₅₀	2.17 μM Compound: Formononetin	Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 22633834]
SH-SY5Y	IC₅₀	> 100 μM Compound: Formononetin	Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay	[PMID: 22633834]
U-937	IC₅₀	44 μM Compound: 6, formononetin	Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	45 μM Compound: 6, formononetin	Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	50 μM Compound: 6, formononetin	Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay	[PMID: 17158054]

In Vitro

Formononetin is one of the major isoflavonoid constituents isolated from Astragalus membranaceus and has been demonstrated diverse pharmacological benefits. Formononetin possesses anti-angiogenic activity in human colon cancer cells. Formononetin also promotes cell cycle arrest via downregulation of Akt/Cyclin D1/CDK4 in human prostate cancer cells^[1].
Formononetin (25 to 150 μM) markedly decreases the proliferation of endothelial cells stimulated by FGF2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HUVECs
Concentration:	0, 10, 25, 50, 75, 100, and 150 μM
Incubation Time:
Result:	Significantly decreased the proliferation of HUVECs stimulated by FGF2 in a dose-dependent manner, while had little inhibitory effects on HUVECs that were not stimulated by FGF2.

In Vivo

Formononetin dramatically suppresses tumor volumes and the Formononetin-treated group tumor weight are significantly inhibited compared with the vehicle group . Formononetin treatment is well tolerated, and there is no significant difference in weight between vehicle group and formononetin treated groups^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice bearing MDA-MB-231 xenografts^[1]
Dosage:	100 mg/kg
Administration:	Treated daily by intragastric administration for 25 days
Result:	Inhibited breast cancer growth and angiogenesis in vivo.

Molecular Weight

268.26

Formula

C₁₆H₁₂O₄

CAS No.

485-72-3

Appearance

Solid

Color

White to off-white

SMILES

O=C1C(C2=CC=C(OC)C=C2)=COC3=CC(O)=CC=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : ≥ 35 mg/mL (130.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7277 mL	18.6386 mL	37.2773 mL
5 mM	0.7455 mL	3.7277 mL	7.4555 mL
10 mM	0.3728 mL	1.8639 mL	3.7277 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.32 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.32 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (18.64 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 25 mg/mL (93.19 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.21%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (246 KB) HNMR (134 KB) RP-HPLC (257 KB) MS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Xiao Yu Wu,et al. Formononetin, a novel FGFR2 inhibitor, potently inhibits angiogenesis and tumor growth in preclinical models. Oncotarget. 2015 Dec 29;6(42):44563-78. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7277 mL	18.6386 mL	37.2773 mL	93.1932 mL
	5 mM	0.7455 mL	3.7277 mL	7.4555 mL	18.6386 mL
	10 mM	0.3728 mL	1.8639 mL	3.7277 mL	9.3193 mL
	15 mM	0.2485 mL	1.2426 mL	2.4852 mL	6.2129 mL
	20 mM	0.1864 mL	0.9319 mL	1.8639 mL	4.6597 mL
	25 mM	0.1491 mL	0.7455 mL	1.4911 mL	3.7277 mL
	30 mM	0.1243 mL	0.6213 mL	1.2426 mL	3.1064 mL
	40 mM	0.0932 mL	0.4660 mL	0.9319 mL	2.3298 mL
	50 mM	0.0746 mL	0.3728 mL	0.7455 mL	1.8639 mL
	60 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5532 mL
	80 mM	0.0466 mL	0.2330 mL	0.4660 mL	1.1649 mL
	100 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9319 mL

Formononetin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Formononetin485-72-3Biochanin B Flavosil FormononetolFGFRApoptosisFibroblast growth factor receptorAnti-angiogenicanti-cancerangiogenesisbreastcancerFGFR2AktInhibitorinhibitorinhibit

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Formononetin (Synonyms: Biochanin B; Flavosil; Formononetol)

Customer Review

Other Forms of Formononetin:

Formononetin Related Antibodies

24 Publications Citing Use of MCE Formononetin

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

Formononetin Related Antibodies

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Complete Stock Solution Preparation Table

Formononetin Related Classifications

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