1. Protein Tyrosine Kinase/RTK
    Apoptosis
  2. FGFR
    Apoptosis
  3. Formononetin

Formononetin (Synonyms: Biochanin B; Flavosil; Formononetol)

Cat. No.: HY-N0183 Purity: 99.88%
Handling Instructions

Formononetin is a potent FGFR2 inhibitor with an IC50 of ~4.31 μM. Formononetin potently inhibits angiogenesis and tumor growth.

For research use only. We do not sell to patients.

Formononetin Chemical Structure

Formononetin Chemical Structure

CAS No. : 485-72-3

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10 mM * 1 mL in DMSO USD 55 In-stock
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Based on 7 publication(s) in Google Scholar

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Description

Formononetin is a potent FGFR2 inhibitor with an IC50 of ~4.31 μM. Formononetin potently inhibits angiogenesis and tumor growth[1].

IC50 & Target[1]

FGFR2

4.31 μM (IC50)

In Vitro

Formononetin is one of the major isoflavonoid constituents isolated from Astragalus membranaceus and has been demonstrated diverse pharmacological benefits. Formononetin possesses anti-angiogenic activity in human colon cancer cells. Formononetin also promotes cell cycle arrest via downregulation of Akt/Cyclin D1/CDK4 in human prostate cancer cells[1].
Formononetin (25 to 150 μM) markedly decreases the proliferation of endothelial cells stimulated by FGF2[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HUVECs
Concentration: 0, 10, 25, 50, 75, 100, and 150 μM
Incubation Time:
Result: Significantly decreased the proliferation of HUVECs stimulated by FGF2 in a dose-dependent manner, while had little inhibitory effects on HUVECs that were not stimulated by FGF2.
In Vivo

Formononetin dramatically suppresses tumor volumes and the Formononetin-treated group tumor weight are significantly inhibited compared with the vehicle group . Formononetin treatment is well tolerated, and there is no significant difference in weight between vehicle group and formononetin treated groups[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice bearing MDA-MB-231 xenografts[1]
Dosage: 100 mg/kg
Administration: Treated daily by intragastric administration for 25 days
Result: Inhibited breast cancer growth and angiogenesis in vivo.
Molecular Weight

268.26

Formula

C₁₆H₁₂O₄

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 35 mg/mL (130.47 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7277 mL 18.6386 mL 37.2773 mL
5 mM 0.7455 mL 3.7277 mL 7.4555 mL
10 mM 0.3728 mL 1.8639 mL 3.7277 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (9.32 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (9.32 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.32 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.88%

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Formononetin
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HY-N0183
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