Cat. No.: HY-121833

Gambogic amide

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Based on 1 Customer Validation

Gambogic amide is a potent and selective agonist of TrkA and also induces its tyrosine phosphorylation and activation of downstream signaling, including Akt and MAPK. Gambogic amide specifically interacts with the cytoplasmic juxtamembrane domain of the TrkA receptor and triggers its dimerization, leading to activation. Gambogic amide has neuroprotective activity preventing glutamate-induced neuronal cell death. Gambogic amide has improved efficacy in a transient middle cerebral artery occlusion model of stroke and could be used to study neurodegenerative diseases and stroke.

For research use only. We do not sell to patients.

Purity: 95.76%
CAS No.: 286935-60-2
Formula: C₃₈H₄₅NO₇
Molecular Weight:627.77

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Gambogic amide Chemical Structure

CAS No.: 286935-60-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Trk Receptor Isoforms :

All Akt Isoforms :

All ERK Isoforms :

In Vitro

Gambogic amide (0.5 μM; 30 min) elicits TrkA tyrosine phosphorylation in hippocampal neurons^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Hippocampal neurons
Concentration:

500 nM
Incubation Time:

30 min
Result:

Triggered TrkA Y490 phosphorylation.

In Vivo

Gambogic amide (2 mg/kg; sc; single dose) prevents neuronal cell death and decreases infarct volume in MCAO animal model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

MCAO mice model^[1]
Dosage:

2 mg/kg
Administration:

s.c.; 5 min before the onset of reperfusion, after 2 h of MCAO followed by reperfusion; followed by 25 mg/kg Kainic acid (KA)
Result:

Diminished Kainic acid-triggered hippocampal neuronal cell death.
Reduces infarct volume in MCAO rat brain.

Chemical Information

CAS No. 286935-60-2
Appearance Solid
Molecular Weight 627.77
Formula C₃₈H₄₅NO₇
Color Light yellow to yellow
SMILES

NC(/C(C)=C\C[C@@]12[C@]34C(C(C5=C(O)C(C=C[C@](CC/C=C(C)/C)(C)O6)=C6C(C/C=C(C)\C)=C5O3)=O)=C[C@H](C[C@H]4C(C)(C)O2)C1=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 68 mg/mL (108.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5929 mL

7.9647 mL

15.9294 mL

5 mM

0.3186 mL

1.5929 mL

3.1859 mL

10 mM

0.1593 mL

0.7965 mL

1.5929 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jang SW, et al. Gambogic amide, a selective agonist for TrkA receptor that possesses robust neurotrophic activity, prevents neuronal cell death. Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16329-34. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5929 mL	7.9647 mL	15.9294 mL	39.8235 mL
	5 mM	0.3186 mL	1.5929 mL	3.1859 mL	7.9647 mL
	10 mM	0.1593 mL	0.7965 mL	1.5929 mL	3.9824 mL
	15 mM	0.1062 mL	0.5310 mL	1.0620 mL	2.6549 mL
	20 mM	0.0796 mL	0.3982 mL	0.7965 mL	1.9912 mL
	25 mM	0.0637 mL	0.3186 mL	0.6372 mL	1.5929 mL
	30 mM	0.0531 mL	0.2655 mL	0.5310 mL	1.3275 mL
	40 mM	0.0398 mL	0.1991 mL	0.3982 mL	0.9956 mL
	50 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7965 mL
	60 mM	0.0265 mL	0.1327 mL	0.2655 mL	0.6637 mL
	80 mM	0.0199 mL	0.0996 mL	0.1991 mL	0.4978 mL
	100 mM	0.0159 mL	0.0796 mL	0.1593 mL	0.3982 mL

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Gambogic amide

Bioactive Compound Library Max

Bioactive Compound Library

Immunology/Inflammation Compound Library

MAPK Compound Library

Neuronal Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Glutamine Metabolism Compound Library

Neuroprotective Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

Antidepressant Compound Library

Osteogenesis Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Activators Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Covalent Screening Library Plus

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

All Trk Receptor Isoforms

All Akt Isoforms

All ERK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Gambogic amide Related Classifications

HY-121833 Related Classifications