NCR247

NCR247 (~116-120 s) binds haem with nanomolar affinity, with cysteine residues, the C₁₀P₁₁ motif, and tyrosine residues contributing to high-affinity binding; the D-enantiomer and M. sativa ortholog retain equivalent nanomolar haem-binding affinity^[1].
NCR247 (5 μM; 16 min) sequesters haem and inhibits its intrinsic peroxidase activity, rendering the haem biologically inaccessible^[1].
NCR247 (2-4 μM; 30-60 min) induces an iron starvation response in S. meliloti by increasing expression of iron uptake genes and reducing expression of rirA; this response requires uptake of NCR247 into the bacterial cytoplasm via BacA and is mediated by the Irr regulatory pathway^[1].
NCR247 (2 μM; 30-60 min) drives increased iron import into S. meliloti, resulting in elevated total cellular iron content; this response is dependent on the Irr regulatory pathway^[1].
NCR247 (25-150 μM; 6 h) inhibit Toxoplasma gondii plaque formation on human foreskin fibroblasts in a concentration-dependent manner, with haem binding required for the activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.