2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. Hcyb1

Hcyb1 is a highly selective, orally active PDE2 inhibitor. Hcyb1 has a highly selective inhibition of PDE2A (IC50=0.57 μM) and over 250-fold selectivity against other recombinant PDE family members. Hcyb1 produces neuroprotective and antidepressant‐like effects most likely mediated by cAMP/cGMP-CREB-BDNF signaling.

For research use only. We do not sell to patients.

Hcyb1 Chemical Structure

Hcyb1 Chemical Structure

CAS No. : 2988566-71-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 330 In-stock
Solution
10 mM * 1 mL in DMSO USD 330 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 950 In-stock
50 mg USD 1450 In-stock
100 mg USD 2250 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Hcyb1 Related Antibodies

Top Publications Citing Use of Products

View All Phosphodiesterase (PDE) Isoform Specific Products:

View All Isoforms
PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

  • Biological Activity

  • Purity & Documentation

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Description

Hcyb1 is a highly selective, orally active PDE2 inhibitor. Hcyb1 has a highly selective inhibition of PDE2A (IC50=0.57 μM) and over 250-fold selectivity against other recombinant PDE family members. Hcyb1 produces neuroprotective and antidepressant‐like effects most likely mediated by cAMP/cGMP-CREB-BDNF signaling[1].

IC50 & Target

PDE2

 

In Vitro

Hcyb1 (1~100 nM; 10 minutes) increases cGMP levels by 1.7~2.3 folds[1].
Hcyb1 (1 nM; 24 hours) increases both cGMP and cAMP levels[1].
Hcyb1 (24 hours) treatment also increases the levels of phosphorylation of CREB and BDNF in HT-22 cells[1].
Hcyb1 promotes HT-22 cell viability and increase the cGMP and cAMP accumulation in HT-22 cells[1].
Hcyb1 exhibits the concentration- and time-dependent effects on cell viability in HT-22 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hcyb1 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: HT-22 cells
Concentration: 1 pM, 0.01 nM, 0.1 nM, 1 nM, 0.01 μM, 0.1 μM, 1 μM, 10 μM
Incubation Time: 24 hours
Result: The cell viability was significantly increased when treatment HT-22 cells with Hcyb1 at concentrations of 0.1 nM and 1 nM for 24 hours. The time-dependent effects showed that the cell viability was significantly increased from 12 to 24 hours when treatment at concentration of 1 nM. The maximal effects peaked at 24 hours after treatment.

Western Blot Analysis[1]

Cell Line: HT-22 cells
Concentration: 1 nM
Incubation Time: 24 hours
Result: Induced a significant increase in the phosphorylation of CREB. BDNF expression was also significantly upregulated at the same concentration.
In Vivo

Hcyb1 (0.5, 1, and 2 mg/kg, i.g.) decreases the immobility time in both forced swimming and tail suspension tests, without altering locomotor activity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male imprinting control region (ICR) mice, weighing between 20 and 25 g[3]
Dosage: 0.5, 1, and 2 mg/kg
Administration: Gavage (i.g.)
Result: Exhibited dose-dependent reduction in immobility time at doses of 0.5, 1, 2 mg/kg (i.g.).
Molecular Weight

380.44

Formula

C24H20N4O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1NC(CC2=CC=CC=C2)=NC3=C1N=CN3C(C)C4=C5C=CC=CC5=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (164.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6285 mL 13.1427 mL 26.2854 mL
5 mM 0.5257 mL 2.6285 mL 5.2571 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (5.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6285 mL 13.1427 mL 26.2854 mL 65.7134 mL
5 mM 0.5257 mL 2.6285 mL 5.2571 mL 13.1427 mL
10 mM 0.2629 mL 1.3143 mL 2.6285 mL 6.5713 mL
15 mM 0.1752 mL 0.8762 mL 1.7524 mL 4.3809 mL
20 mM 0.1314 mL 0.6571 mL 1.3143 mL 3.2857 mL
25 mM 0.1051 mL 0.5257 mL 1.0514 mL 2.6285 mL
30 mM 0.0876 mL 0.4381 mL 0.8762 mL 2.1904 mL
40 mM 0.0657 mL 0.3286 mL 0.6571 mL 1.6428 mL
50 mM 0.0526 mL 0.2629 mL 0.5257 mL 1.3143 mL
60 mM 0.0438 mL 0.2190 mL 0.4381 mL 1.0952 mL
80 mM 0.0329 mL 0.1643 mL 0.3286 mL 0.8214 mL
100 mM 0.0263 mL 0.1314 mL 0.2629 mL 0.6571 mL
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Hcyb1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hcyb12988566-71-6Hcyb 1Hcyb-1Phosphodiesterase (PDE)antidepressantPDE2 inhibitorDepressionCREBBDNFPKA/PKGInhibitorinhibitorinhibit

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