iE-DAP dihydrochloride
Based on 1 Customer Validation
iE-DAP dihydrochloride is a Nod1 agonist. iE-DAP dihydrochloride activates NOD1, which in turn activates the NF-κB signaling pathway and MLCK signaling pathway, inducing cellular inflammatory responses and tight junction disruption. iE-DAP dihydrochloride downregulates the expression of ZO-1 and Occludin genes. iE-DAP dihydrochloride increases the secretion of IL-6, GRO-α, MCP-1, IL-8 and MIP-1β in term human trophoblast cell cultures. iE-DAP dihydrochloride triggers preterm birth in pregnant mice, reduces fetal body weight, and induces fetal inflammation. iE-DAP dihydrochloride can be used in studies related to mastitis and preterm birth.
For research use only. We do not sell to patients.
- Formula: C12H23Cl2N3O7
- Molecular Weight:392.23
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
iE-DAP dihydrochloride (10-10000 ng/mL; 1-24 h) upregulates the mRNA expression of IL-1β, IL-6 and IL-8 in BMECs, downregulates the mRNA expression of ZO-1 and Occludin in BMECs, reduces TEER, and increases the paracellular dextran permeability in BMECs[1].
iE-DAP dihydrochloride (1000 ng/mL; 12 h) upregulates the protein expression levels of p-MLC2 and MLCK as well as the phosphorylation level of MLC2, downregulates the mRNA and protein expression levels of ZO-1 and Occludin, reduces TEER, increases the dextran permeability via the paracellular pathway, and disrupts the distribution of ZO-1 in BMECs[1].
iE-DAP dihydrochloride (1000 ng/mL; 12 h) upregulates the mRNA expression and secretion levels of IL-1β, IL-6 and IL-8 in BMECs, and activates the NF-κB pathway by increasing the expression of p-p65 protein, the phosphorylation level of p65 and the nuclear translocation of p65[1].
iE-DAP dihydrochloride (1000 ng/mL; 12 h) upregulates the expressions of NOD1 protein and p-p65 protein, enhances p65 phosphorylation level, and induces p65 nuclear translocation to activate the NOD1/NF-κB pathway[1].
iE-DAP dihydrochloride (100 μg/mL; 72 h) potently stimulates the secretion of IL-6, GRO-α, MCP-1, IL-8 and MIP-1β by human primary third-trimester cytotrophoblast cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:bovine mammary epithelial cells (BMECs)
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Concentration:1000 ng/mL
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Incubation Time:1, 3, 6, 12, 24 h
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Result:Significantly upregulated IL-1β mRNA relative expression at 1 h.
Significantly upregulated IL-6 mRNA relative expression at 1 h.
Significantly upregulated IL-8 mRNA relative expression at 1 h, with all inflammatory cytokines increasing in a time-dependent manner.
Significantly downregulated ZO-1 mRNA relative expression at 6 h.
Significantly downregulated Occludin mRNA relative expression at 12 h, with both tight junction proteins decreasing in a time-dependent manner.
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Cell Line:bovine mammary epithelial cells (BMECs)
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Concentration:0, 1, 10, 100, 1000, 10000 ng/mL
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Incubation Time:12 h
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Result:Increased the mRNA relative expression and release of IL-1β at 10 ng/mL.
Increased the mRNA relative expression and release of IL-6 and IL-8 at 100 ng/mL.
Increased the gene expression and release of inflammatory cytokines in a concentration-dependent manne.
Reduced the relative mRNA expression of ZO-1、Occludin at 100、1000 ng/mL.
Decreased the gene expression of TJ proteins in a concentration-dependent manne.
Decreased the TEER and increased
paracellular dextran passage at 100 ng/mL.
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Cell Line:bovine mammary epithelial cells (BMECs)
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Concentration:1000 ng/mL
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Incubation Time:12 h
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Result:Increased p-MLC2 protein expression and MLC2 phosphorylation, reduced protein expression of ZO-1 and Occludin, decreases TEER.
Significantly increased p-p65 protein expression and the p-p65/p65 phosphorylation ratio.
Significantly increased NOD1 protein expression.
Significantly increased MLCK protein expression.
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Cell Line:bovine mammary epithelial cells (BMECs)
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Concentration:1000 ng/mL
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Incubation Time:12 h
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Result:Caused redistribution of ZO-1 protein.\nInduced nuclear translocation of NF-κB p65.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J mice (adult, 8-12 wk old, pregnant, preterm labor model induced at embryonic day 14.5)[2]
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Dosage:500 μg; 750 μg; 1000 μg
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Administration:i.p.; single dose
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Result:Induced preterm delivery within 24 hours in 100% of treated mice (1000 μg dose).
Reduced fetal weight, significantly increased placental Eotaxin levels, significantly decreased placental MCP-1 levels, significantly increased decidual IL-1α and RANTES levels, and triggered a significant fetal proinflammatory response with elevated levels of Eotaxin, IL-1β, IL-3, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17, G-CSF, GM-CSF, MIP-1α, MIP-1β, KC, TNF-α, and IFN-γ (750 μg dose).
Did not induce preterm delivery (500 μg dose).
Chemical Information
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Appearance Solid
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Molecular Weight 392.23
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Formula C12H23Cl2N3O7
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Color White to off-white
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Sequence
γ-d-Glu-meso-DAP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (254.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang Y, et al. iE-DAP Induced Inflammatory Response and Tight Junction Disruption in Bovine Mammary Epithelial Cells via NOD1-Dependent NF-κB and MLCK Signaling Pathway. Int J Mol Sci. 2023;24(7):6263. Published 2023 Mar 27. [Content Brief]
[2]. Cardenas I, et al. Nod1 activation by bacterial iE-DAP induces maternal-fetal inflammation and preterm labor. J Immunol. 2011;187(2):980-986. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5495 mL | 12.7476 mL | 25.4952 mL | 63.7381 mL |
| 5 mM | 0.5099 mL | 2.5495 mL | 5.0990 mL | 12.7476 mL | |
| 10 mM | 0.2550 mL | 1.2748 mL | 2.5495 mL | 6.3738 mL | |
| 15 mM | 0.1700 mL | 0.8498 mL | 1.6997 mL | 4.2492 mL | |
| 20 mM | 0.1275 mL | 0.6374 mL | 1.2748 mL | 3.1869 mL | |
| 25 mM | 0.1020 mL | 0.5099 mL | 1.0198 mL | 2.5495 mL | |
| 30 mM | 0.0850 mL | 0.4249 mL | 0.8498 mL | 2.1246 mL | |
| 40 mM | 0.0637 mL | 0.3187 mL | 0.6374 mL | 1.5935 mL | |
| 50 mM | 0.0510 mL | 0.2550 mL | 0.5099 mL | 1.2748 mL | |
| 60 mM | 0.0425 mL | 0.2125 mL | 0.4249 mL | 1.0623 mL | |
| 80 mM | 0.0319 mL | 0.1593 mL | 0.3187 mL | 0.7967 mL | |
| 100 mM | 0.0255 mL | 0.1275 mL | 0.2550 mL | 0.6374 mL |
iE-DAP dihydrochloride Related Classifications
HY-P5523A Related Classifications
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