Cat. No.: HY-N4187

Licochalcone D

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Licochalcone D, a flavonoid compound mainly existing in the root of Glycyrrhiza uralensis, is a potent and orally active inhibitor of NF-kappaB (NF-κB) p65. Licochalcone D possesses antioxidant, anti-inflammatory, anti-cancer properties.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 144506-15-0
Formula: C₂₁H₂₂O₅
Molecular Weight:354.40

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Licochalcone D Chemical Structure

CAS No.: 144506-15-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

p65

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	4.85 μM Compound: 4, LicoD	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production after 18 hrs by Griess assay	[PMID: 24316124]
RBL-2H3	IC₅₀	21 μM Compound: 4, LicoD	Antiinflammatory activity in RBL2H3 cells assessed as inhibition of degranulation Antiinflammatory activity in RBL2H3 cells assessed as inhibition of degranulation	[PMID: 24316124]

In Vitro

Licochalcone D (10 μM; 1 h) negatively regulates Lipopolysaccharides (LPS) (HY-D1056)-induced NF-κB activation by inhibiting the phosphorylation and transactivation of NF-κB p65, inhibits the LPS-induced activation of protein kinase A (PKA), and significantly inhibits the LPS-induced expression of TNFα and MCP-1^[1].
? Licochalcone D (10 μM; 24 h) significantly inhibits LPS-induced iNOS expression and NO production^[1].
? Licochalcone D (0-90 μM; 24 h) inhibits the proliferation of human melanoma cells, induces the apoptosis of A375 cells, decreases the mitochondrial membrane potential and increases ROS production in A375 cells^[2].
? Licochalcone D (0-25 μM; 24 h) decreases the migration and invasion ability of the A375 cells, decreased the protein levels of MMP-2 and MMP-9^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW264.7 cells
Concentration:

10 μM
Incubation Time:

1 h or 24 h (iNOS), following LPS stimulation (1 μg/mL) for 30 min
Result:

Inhibited the phosphorylation of NF-κB at serine 276 but not at serine 536.
Inhibited the LPS-induced expression of TNFα mRNA and MCP-1 mRNA.
Strongly inhibited the expression of iNOS.

Cell Viability Assay^[2]

Cell Line:

A375 cells or SK-MEL-5
Concentration:

0, 1, 2.5, 5, 15, 30, 45, 60, 75 and 90 μM (A375) or 0, 20, 40, 60 and 80 μM (SK-MEL-5)
Incubation Time:

24 h
Result:

Inhibited the proliferation of A375 and SK-MEL-5 in a concentration dependent manner.

Apoptosis Analysis^[2]

Cell Line:

A375
Concentration:

0, 30, 60 and 90 μM
Incubation Time:

24 h
Result:

Cells exhibited obvious apoptotic characteristics after the treatment.

RT-PCR^[2]

Cell Line:

A375
Concentration:

0, 30, 60 and 90 μM
Incubation Time:

24 h
Result:

Downregulated the mRNA level of Bcl-2 and upregulated the mRNA levels of caspase-3, caspase-9 and Bax.

Cell Invasion Assay^[2]

Cell Line:

A375
Concentration:

25 μM
Incubation Time:

24 h
Result:

Significantly suppressed cell invasion.

Cell Migration Assay ^[2]

Cell Line:

A375
Concentration:

25 μM
Incubation Time:

24 h
Result:

Significantly suppressed cell migration in the A375 cells.

In Vivo

Licochalcone D (25 and 50 mg/kg; i.g.; daily for a week) inhibits the tumor growth in a mouse xenograft model of murine melanoma B16F0 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 mice, B16F0 tumor model^[2]
Dosage:

25 and 50 mg/kg
Administration:

Intragastric administration, daily for a week
Result:

Obviously lowered the tumor growth rates, the tumor growth inhibition rates were calculated to be 32.0 and 54.1% at 25 and 50 mg/kg, respectively.

Chemical Information

CAS No. 144506-15-0
Appearance Solid
Molecular Weight 354.40
Formula C₂₁H₂₂O₅
Color Light yellow to yellow
SMILES

O=C(C1=CC=C(O)C(C/C=C(C)\C)=C1)/C=C/C2=CC=C(O)C(O)=C2OC
Structure Classification
- Flavonoids
- Chalcones
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

All Cell Proliferation/Viability AssayCell Imaging/StainingWBHistological Imaging/StainingRT-PCR

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Journal Impact Factor

Sci China Life Sci

Targeting STING oligomerization with licochalcone D ameliorates STING-driven inflammatory diseases. [Abstract]

2024 Dec;67(12):2664-2677. PMID: 39190126
Int Immunopharmacol

Licochalcone D modulates macrophage ferroptosis via suppression of VPS4A-mediated lipophagy to alleviate MRSA pneumonia. [Abstract]

2026 Jan 1;168(Pt 2):115918. PMID: 41275832
Int J Mol Sci

Licochalcone D from Glycyrrhiza uralensis Improves High-Glucose-Induced Insulin Resistance in Hepatocytes. [Abstract]

2024 Sep 19;25(18):10066. PMID: 39337550

Licochalcone D purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Sep 19;25(18):10066. [Abstract]

The cell viability under Licochalcone D (LicoD) (0.1, 0.5, 1, 2, 5, 10 μM) treatment was measured using a water-soluble tetrazolium salt-1 kit,

Licochalcone D purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Sep 19;25(18):10066. [Abstract]

Expression levels of p-IRS1 (Ser307), p-IRS1 (Ser1011), t-IRS1, p-PI3K (Tyr458/Tyr199), t-PI3K, and GAPDH were analyzed using Western blot assay treated with Licochalcone D (LicoD) (2 μM).

Licochalcone D purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Sep 19;25(18):10066. [Abstract]

mRNA expression of Pepck and G6pase treated with Licochalcone D (LicoD) (2 μM).
Biomolecules

Licochalcone E, a β-Amyloid Aggregation Inhibitor, Regulates Microglial M1/M2 Polarization via Inhibition of CTL1-Mediated Choline Uptake. [Abstract]

2023 Jan 17;13(2):191. PMID: 36830561
J Orthop Surg Res

Licochalcone D inhibits osteoclast differentiation and postmenopausal osteoporosis by inactivating the NF-κB signaling pathway. [Abstract]

2025 Jul 28;20(1):713. PMID: 40722101

Licochalcone D purchased from MedChemExpress. Usage Cited in: J Orthop Surg Res. 2025 Jul 28;20(1):713. [Abstract]

TRAP staining was performed to measure the number of osteoclasts in control, RANKL + Licochalcone D (Lico D) (2, 4, 8 μg/L), and RANKL + BAY 11-7821 groups.

Licochalcone D purchased from MedChemExpress. Usage Cited in: J Orthop Surg Res. 2025 Jul 28;20(1):713. [Abstract]

H&E staining was performed to evaluate bone loss in the sham, model, model + Licochalcone D (Lico D-L) (10 mg/kg, s.c.), and model + Lico D-H (50 mg/kg, s.c.) groups.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (282.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8217 mL

14.1084 mL

28.2167 mL

5 mM

0.5643 mL

2.8217 mL

5.6433 mL

10 mM

0.2822 mL

1.4108 mL

2.8217 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Furusawa J, et al. Glycyrrhiza inflata-derived chalcones, Licochalcone A, Licochalcone B and Licochalcone D, inhibit phosphorylation of NF-kappaB p65 in LPS signaling pathway. Int Immunopharmacol. 2009 Apr;9(4):499-507. [Content Brief]

[2]. Si L, et al. Licochalcone D induces apoptosis and inhibits migration and invasion in human melanoma A375 cells. Oncol Rep. 2018 May;39(5):2160-2170. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8217 mL	14.1084 mL	28.2167 mL	70.5418 mL
	5 mM	0.5643 mL	2.8217 mL	5.6433 mL	14.1084 mL
	10 mM	0.2822 mL	1.4108 mL	2.8217 mL	7.0542 mL
	15 mM	0.1881 mL	0.9406 mL	1.8811 mL	4.7028 mL
	20 mM	0.1411 mL	0.7054 mL	1.4108 mL	3.5271 mL
	25 mM	0.1129 mL	0.5643 mL	1.1287 mL	2.8217 mL
	30 mM	0.0941 mL	0.4703 mL	0.9406 mL	2.3514 mL
	40 mM	0.0705 mL	0.3527 mL	0.7054 mL	1.7635 mL
	50 mM	0.0564 mL	0.2822 mL	0.5643 mL	1.4108 mL
	60 mM	0.0470 mL	0.2351 mL	0.4703 mL	1.1757 mL
	80 mM	0.0353 mL	0.1764 mL	0.3527 mL	0.8818 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7054 mL

Keywords

Licochalcone D

5 Cited Publications

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Licochalcone D

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

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Publications Citing Use of MedChemExpress (MCE) Licochalcone D

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Licochalcone D Related Classifications

HY-N4187 Related Classifications