Tubercidin
Based on 8 publication(s) in Google Scholar
Tubercidin (7-Deazaadenosine) is an antibiotic obtained from Streptomyces tubercidicus. Tubercidin inhibits the growth of Streptococcus faecalis (8043) with an IC50 of 0.02 μM. Tubercidin inhibits polymerases by incorporating DNA or RNA, thereby inhibiting DNA replication, RNA and protein synthesis. Tubercidin is a weak inhibitor of adenosine phosphorylase, and interferes with the phosphorylation of adenosine and AMP. Tubercidin has antiviral activity.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.24%
- CAS 番号: 69-33-0
- 分子式: C11H14N4O4
- 分子量:266.26
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
MedChemExpress(MCE)の使用を引用している文献 Tubercidin
More- Elife. 2022 Dec 7:11:e79116. [Abstract]
- Microb Cell Fact. 2018 Aug 28;17(1):131. [Abstract]
- J Med Virol. 2025 Oct;97(10):e70655. [Abstract]
- Microbiol Spectr. 2024 Mar 5;12(3):e0347923. [Abstract]
- J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Jan 1:1232:123968. [Abstract]
- Res Sq. 2026 Mar 5.
- bioRxiv. 2025 July 11.
- bioRxiv. 2024 July 27.
DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示
MoreAntibiotic アイソフォーム固有の製品をすべて表示
More
生物活性
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | IC50 |
0.043 μM
Compound: Tubercidin
|
Cytotoxicity against human A-375 cells assessed as cell growth inhibition
Cytotoxicity against human A-375 cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| A549 | GI50 |
0.001 μM
Compound: Tubercidin
|
Cytostatic activity against human A549 cells after 5 days by SRB assay
Cytostatic activity against human A549 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| A549 | IC50 |
0.044 μM
Compound: Tubercidin
|
Cytotoxicity against human A549 cells assessed as cell growth inhibition
Cytotoxicity against human A549 cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| CCRF-CEM | IC50 |
0.11 μM
Compound: 1; 1a; Tub
|
Cytotoxicity against human CCRF-CEM cells assessed as cell growth inhibition
Cytotoxicity against human CCRF-CEM cells assessed as cell growth inhibition
|
[PMID: 35763869] |
| DU-145 | GI50 |
0.018 μM
Compound: Tubercidin
|
Cytostatic activity against human DU145 cells after 5 days by SRB assay
Cytostatic activity against human DU145 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| DU-145 | IC50 |
0.256 μM
Compound: Tubercidin
|
Cytotoxicity against human DU-145 cells assessed as cell growth inhibition
Cytotoxicity against human DU-145 cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| HCT-116 | GI50 |
0.001 μM
Compound: Tubercidin
|
Cytostatic activity against human HCT116 cells after 5 days by SRB assay
Cytostatic activity against human HCT116 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| HCT-116 | IC50 |
0.082 μM
Compound: Tubercidin
|
Cytotoxicity against human HCT-116 cells assessed as cell growth inhibition
Cytotoxicity against human HCT-116 cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| HCT-15 | GI50 |
0.011 μM
Compound: Tubercidin
|
Cytostatic activity against human HCT15 cells after 5 days by SRB assay
Cytostatic activity against human HCT15 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| HEK293 | IC50 |
0.88 μM
Compound: 4
|
Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay
Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay
|
[PMID: 21870828] |
| HeLa S3 | IC50 |
0.03 μM
Compound: 3
|
Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days
Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days
|
[PMID: 20964406] |
| HeLa S3 | IC50 |
0.4 μM
Compound: 3
|
Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay
Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay
|
[PMID: 20964406] |
| HeLa S3 | IC50 |
0.819 μM
Compound: 3
|
Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay
Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay
|
[PMID: 20964406] |
| HeLa S3 | IC50 |
1.44 μM
Compound: 3
|
Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay
Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay
|
[PMID: 20964406] |
| HeLa S3 | IC50 |
0.1 μM
Compound: 1; 1a; Tub
|
Cytotoxicity against human HeLa S3 cells assessed as cell growth inhibition
Cytotoxicity against human HeLa S3 cells assessed as cell growth inhibition
|
[PMID: 35763869] |
| HEp-2 | IC50 |
>3 μM
Compound: 4
|
Compound was evaluated for cytotoxicity against H.Ep.-2 (AK-) cells and concentration required to inhibit the growth of treated cells to 50% of untreated control
Compound was evaluated for cytotoxicity against H.Ep.-2 (AK-) cells and concentration required to inhibit the growth of treated cells to 50% of untreated control
|
[PMID: 6708054] |
| HEp-2 | IC50 |
0.002 μM
Compound: 4
|
Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control.
Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control.
|
[PMID: 6708054] |
| HepG2 | IC50 |
0.05 μM
Compound: 1; 1a; Tub
|
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition
|
[PMID: 35763869] |
| HFF | IC50 |
0.4 μM
Compound: 1 tubercidin
|
Cytotoxicity against uninfected human foreskin fibroblast(HFF cells)
Cytotoxicity against uninfected human foreskin fibroblast(HFF cells)
|
[PMID: 2846837] |
| HFF | IC50 |
0.3 μM
Compound: 8a
|
Cytotoxicity was evaluated against the Human diploid cells (HFF)
Cytotoxicity was evaluated against the Human diploid cells (HFF)
|
[PMID: 2913300] |
| HFF | IC50 |
0.79 μM
Compound: Tubercidin
|
Cytotoxicity against human HFF cells assessed as cell growth inhibition
Cytotoxicity against human HFF cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| HFF | IC50 |
0.4 μM
Compound: 1a
|
Tested for cytotoxicity in human foreskin fibroblasts at time of HCMV plaque enumeration
Tested for cytotoxicity in human foreskin fibroblasts at time of HCMV plaque enumeration
|
[PMID: 8254613] |
| HL-60 | IC50 |
0.14 μM
Compound: 1; 1a; Tub
|
Cytotoxicity against human HL-60 cells assessed as cell growth inhibition
Cytotoxicity against human HL-60 cells assessed as cell growth inhibition
|
[PMID: 35763869] |
| KB | IC50 |
1 μM
Compound: 1
|
Cytotoxicity of compound was determined by assaying cell growth in human neoplastic cell line(KB).
Cytotoxicity of compound was determined by assaying cell growth in human neoplastic cell line(KB).
|
[PMID: 1310744] |
| KB | IC50 |
0.6 μM
Compound: 1 tubercidin
|
Cytotoxicity against human neoplastic cell line(KB cells)
Cytotoxicity against human neoplastic cell line(KB cells)
|
[PMID: 2846837] |
| KB | IC50 |
0.7 μM
Compound: 8a
|
Cytotoxicity was evaluated in Human neoplastic cell line (KB)
Cytotoxicity was evaluated in Human neoplastic cell line (KB)
|
[PMID: 2913300] |
| KB | IC50 |
1 μM
Compound: 1a
|
Tested for the inhibition against KB cell growth
Tested for the inhibition against KB cell growth
|
[PMID: 8254613] |
| L1210 | IC50 |
0.04 μM
Compound: 1
|
Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro.
Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro.
|
[PMID: 1310744] |
| L1210 | IC50 |
4 x 10-8 M
Compound: 8a
|
In vitro cytotoxicity was evaluated against the L1210 Murine leukemic cells
In vitro cytotoxicity was evaluated against the L1210 Murine leukemic cells
|
[PMID: 2913300] |
| L1210 | IC50 |
0.018 μg/mL
Compound: 11
|
In vitro inhibition of L1210 (murine leukemia) cell growth.
In vitro inhibition of L1210 (murine leukemia) cell growth.
|
[PMID: 6538600] |
| L1210 | IC50 |
6.77 x 10-8 M
Compound: 11
|
In vitro inhibition of L1210 (murine leukemia) cell growth.
In vitro inhibition of L1210 (murine leukemia) cell growth.
|
[PMID: 6538600] |
| L1210 | IC50 |
0.043 μM
Compound: 1a
|
Tested for in vitro cell growth inhibition of L1210 cells
Tested for in vitro cell growth inhibition of L1210 cells
|
[PMID: 8254613] |
| L1210 | IC50 |
0.043 μM
Compound: 5 (tubercidin)
|
Cytotoxicity in L1210 cell culture.
Cytotoxicity in L1210 cell culture.
|
[PMID: 9057866] |
| Macrophage | CC50 |
0.13 μM
Compound: Tubercidin
|
Cytotoxicity against Swiss mouse peritoneal macrophages assessed as reduction in cell viability measured after 5 days by microscopic analysis
Cytotoxicity against Swiss mouse peritoneal macrophages assessed as reduction in cell viability measured after 5 days by microscopic analysis
|
[PMID: 35144125] |
| MRC5 | EC50 |
2.23 μM
Compound: 1
|
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay
|
[PMID: 30677668] |
| MRC5 | CC50 |
2.23 μM
Compound: Tubercidin
|
Cytotoxicity against human MRC5 fibroblast cells assessed as reduction in cell viability measured after 3 days by fluorometry
Cytotoxicity against human MRC5 fibroblast cells assessed as reduction in cell viability measured after 3 days by fluorometry
|
[PMID: 35144125] |
| MT4 | GI50 |
0.021 μM
Compound: Tubercidin
|
Cytostatic activity against human MT4 cells after 5 days by SRB assay
Cytostatic activity against human MT4 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| NCI-H23 | GI50 |
0.011 μM
Compound: Tubercidin
|
Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay
Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| NHDF | IC50 |
0.07 μM
Compound: 1; 1a; Tub
|
Cytotoxicity against human NHDF cells assessed as cell growth inhibition
Cytotoxicity against human NHDF cells assessed as cell growth inhibition
|
[PMID: 35763869] |
| PC-3 | GI50 |
0.048 μM
Compound: Tubercidin
|
Cytostatic activity against human PC3 cells after 5 days by SRB assay
Cytostatic activity against human PC3 cells after 5 days by SRB assay
|
[PMID: 21711054] |
| PC-3 | IC50 |
0.076 μM
Compound: Tubercidin
|
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| SK-OV-3 | IC50 |
0.131 μM
Compound: Tubercidin
|
Cytotoxicity against human SK-OV-3 cells assessed as cell growth inhibition
Cytotoxicity against human SK-OV-3 cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| T47D | IC50 |
0.013 μM
Compound: Tubercidin
|
Cytotoxicity against human T47D cells assessed as cell growth inhibition
Cytotoxicity against human T47D cells assessed as cell growth inhibition
|
[PMID: 33132173] |
| Vero | CC50 |
>357 μM
Compound: 4; 7-DMA
|
Cytotoxicity against African green monkey Vero cells by visual method
Cytotoxicity against African green monkey Vero cells by visual method
|
[PMID: 31549836] |
| Vero | EC50 |
1.3 μM
Compound: 4; 7-DMA
|
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay
|
[PMID: 31549836] |
| Vero | EC50 |
1.3 μM
Compound: 4; 7-DMA
|
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay
|
[PMID: 31549836] |
| Vero | EC50 |
1.3 μM
Compound: 4; 7-DMA
|
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield
|
[PMID: 31549836] |
| Vero | EC50 |
1.3 μM
Compound: 4; 7-DMA
|
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay
Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay
|
[PMID: 31549836] |
| Vero C1008 | EC50 |
0.59 μM
Compound: Tubercidin
|
Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in infection measured after 24 hrs by Plaque assay
Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in infection measured after 24 hrs by Plaque assay
|
[PMID: 37229831] |
Tubercidin (7-Deazaadenosine) (0-10 nM; 14 days) has a dose-dependent inhibitory effect on myeloid and erythroid human bone marrow progenitor cells, and the IC50s of tubercidin are 3.4 nM and 3.7 nM for CFU-GM and BFU-E, respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Human bone marrow progenitor cells
-
Concentration:0-10 nM
-
Incubation Time:14 days
-
Result:Had a dose-dependent inhibitory effect for CFU-GM and BFU-E.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Female CD1 mice[2]
-
Dosage:5 mg/kg
-
Administration:Intraperitoneal injection; 5 mg/kg; 10 days
-
Result:Protected the mice from the lethality of tubercidin.
化学情報
-
CAS 番号 69-33-0
-
性状 Solid
-
分子量 266.26
-
分子式 C11H14N4O4
-
Color White to off-white
-
SMILES
O[C@H]1[C@@H](O)[C@H](N2C=CC3=C2N=CN=C3N)O[C@@H]1CO
-
別名
7-Deazaadenosine; ツベルシジン
-
Structure Classification
-
Initial Source
Streptomyces tubercidicus
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
Elife
Overcoming the cytoplasmic retention of GDOWN1 modulates global transcription and facilitates stress adaptation. [Abstract]2022 Dec 7:11:e79116. PMID: 36476745 -
Microb Cell Fact
Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090. [Abstract]2018 Aug 28;17(1):131. PMID: 30153835 -
J Med Virol
Drug Repurposing: In Vitro Evaluation of Simeprevir as a Novel Antiviral Drug Against Severe Fever With Thrombocytopenia Syndrome Virus. [Abstract]2025 Oct;97(10):e70655. PMID: 41117261 -
Microbiol Spectr
Tubercidin inhibits PRRSV replication via RIG-I/NF-κB pathways and interrupting viral nsp2 synthesis. [Abstract]2024 Mar 5;12(3):e0347923. PMID: 38299833 -
J Chromatogr B Analyt Technol Biomed Life Sci
Exploring urinary modified nucleosides as biomarkers for diabetic retinopathy: Development and validation of a ultra performance liquid chromatography-tandem mass spectrometry method. [Abstract]2024 Jan 1:1232:123968. PMID: 38150972 -
-
-
溶剤 & 溶解度
DMSO : ≥ 30 mg/mL (112.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
-
データシート (280 KB)
-
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Portuguese - PT (480 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Bloch A, et al. On the mode of action of 7-deaza-adenosine (Tubercidin). Biochim Biophys Acta. 1967 Mar 29;138(1):10-25. [Content Brief]
[2]. Bergstrom DE, et al. Antiviral activity of C-5 substituted tubercidin analogues. J Med Chem. 1984 Mar;27(3):285-92. [Content Brief]
[3]. Kouni MH, et al. Prevention of tubercidin host toxicity by nitrobenzylthioinosine 5'-monophosphate for the treatment of schistosomiasis. Antimicrob Agents Chemother. 1989 Jun;33(6):824-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7557 mL | 18.7786 mL | 37.5573 mL | 93.8932 mL |
| 5 mM | 0.7511 mL | 3.7557 mL | 7.5115 mL | 18.7786 mL | |
| 10 mM | 0.3756 mL | 1.8779 mL | 3.7557 mL | 9.3893 mL | |
| 15 mM | 0.2504 mL | 1.2519 mL | 2.5038 mL | 6.2595 mL | |
| 20 mM | 0.1878 mL | 0.9389 mL | 1.8779 mL | 4.6947 mL | |
| 25 mM | 0.1502 mL | 0.7511 mL | 1.5023 mL | 3.7557 mL | |
| 30 mM | 0.1252 mL | 0.6260 mL | 1.2519 mL | 3.1298 mL | |
| 40 mM | 0.0939 mL | 0.4695 mL | 0.9389 mL | 2.3473 mL | |
| 50 mM | 0.0751 mL | 0.3756 mL | 0.7511 mL | 1.8779 mL | |
| 60 mM | 0.0626 mL | 0.3130 mL | 0.6260 mL | 1.5649 mL | |
| 80 mM | 0.0469 mL | 0.2347 mL | 0.4695 mL | 1.1737 mL | |
| 100 mM | 0.0376 mL | 0.1878 mL | 0.3756 mL | 0.9389 mL |