Ursodeoxycholic acid
Based on 26 publication(s) in Google Scholar
Ursodeoxycholic acid (Ursodeoxycholate) is a secondary bile acid issued from the transformation of (cheno)deoxycholic acid by intestinal bacteria, acting as a key regulator of the intestinal barrier integrity and essential for lipid metabolism. Ursodeoxycholic acid acts as signaling molecule, exerting its effects by interacting with bile acid activated receptors, including G-protein coupled bile acid receptor 5 (TGR5, GPCR19) and the farnesoid X receptor (FXR). Ursodeoxycholic acid can be used for the research of a variety of hepatic and gastrointestinal diseases. Ursodeoxycholic acid also reduces ACE2 expression and is beneficial for reducing SARS-CoV-2 infection. Orally active.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.94%
- CAS No.: 128-13-2
- 화학식: C24H40O4
- 분자량:392.57
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) Ursodeoxycholic acid
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
- Nat Commun. 2025 Aug 16;16(1):7638. [Abstract]
- Nat Commun. 2023 May 2;14(1):2523. [Abstract]
- Adv Sci (Weinh). 2025 Oct 27:e15370. [Abstract]
- Adv Sci (Weinh). 2025 Feb 3:e2411719. [Abstract]
- Phytomedicine. 2025 Apr:139:156495. [Abstract]
- Phytomedicine. 2024 Sep:132:155886. [Abstract]
- Free Radic Biol Med. 2020 May 20;152:821-837. [Abstract]
- Food Res Int. 2026 Feb 17.
- J Transl Med. 2024 Dec 20;22(1):1124. [Abstract]
- JCI Insight. 2025 Sep 23;10(18):e190716. [Abstract]
- Cell Prolif. 2023 Nov;56(11):e13488. [Abstract]
- J Ethnopharmacol. 2026 May 23:363:121421. [Abstract]
- Cells. 2022 Sep 21;11(19):2951. [Abstract]
- Commun Biol. 2025 Jul 5;8(1):1009. [Abstract]
- Int J Mol Sci. 2024 Jan 11;25(2):919. [Abstract]
- Eur J Pharmacol. 2026 Apr 10:1020:178775. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Mar;1870(3):167029. [Abstract]
- Aquaculture. 2024 Nov 15.
- Vet Microbiol. 2026 May:316:110973. [Abstract]
- Neurosci Lett. 2021 Jan 10;741:135493. [Abstract]
- Lett Drug Des Discov. 2025 Nov 26.
- Clin Neuropharmacol. 2022 Nov-Dec;45(6):168-174. [Abstract]
- Res Sq. 2025 Nov 17.
- Research Square Preprint. 2021 Aug.
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Histological Imaging/Staining
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RT-PCR
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RT-PCR
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WB
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IF
All Endogenous Metabolite Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human A549 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human A549 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| CHO | EC50 |
36.4 μM
Compound: 9, UDCA
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Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
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[PMID: 18307294] |
| COS-1 | EC50 |
>50 μM
Compound: 9, UDCA
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Agonist activity at human FXR expressed in COS1 cells by luciferase assay
Agonist activity at human FXR expressed in COS1 cells by luciferase assay
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[PMID: 18307294] |
| GBM | IC50 |
>50 μM
Compound: 1b, UDCA, ursodeoxycholic acid
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Cytotoxicity against human GBM cells after 24 hrs by neutral red uptake assay
Cytotoxicity against human GBM cells after 24 hrs by neutral red uptake assay
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[PMID: 20381215] |
| HCT-116 | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human HCT-116 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human HCT-116 cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 36439975] |
| HCT-116 | IC50 |
>50 μM
Compound: 1b, UDCA, ursodeoxycholic acid
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Cytotoxicity against human HCT116 cells after 24 hrs by neutral red uptake assay
Cytotoxicity against human HCT116 cells after 24 hrs by neutral red uptake assay
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[PMID: 20381215] |
| HEK293 | EC50 |
2.39 μM
Compound: 3, UDCA
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Agonist activity at human GPBAR1 expressed in HEK293 cells assessed as increase in intracellular cAMP level after 30 mins by cAMP-Glo assay
Agonist activity at human GPBAR1 expressed in HEK293 cells assessed as increase in intracellular cAMP level after 30 mins by cAMP-Glo assay
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[PMID: 25735208] |
| HEK-293T | EC50 |
>150 μM
Compound: UDCA
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Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
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[PMID: 26774929] |
| HEK-293T | IC50 |
>50 μM
Compound: UDCA
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Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
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[PMID: 26774929] |
| HeLa | IC50 |
3.6 μM
Compound: Ursodeoxycholate
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TP_TRANSPORTER: inhibition of Taurocholate uptake in NTCP-expressing HeLa cells
TP_TRANSPORTER: inhibition of Taurocholate uptake in NTCP-expressing HeLa cells
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[PMID: 10565843] |
| HET-1A | CC50 |
1313 μM
Compound: 7, UDCA
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Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 20713311] |
| HT-29 | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human HT-29 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human HT-29 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| Huh-7 | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human Huh-7 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human Huh-7 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| KMS-11 | IC50 |
>50 μM
Compound: 1b, UDCA, ursodeoxycholic acid
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Cytotoxicity against human KMS11 cells after 24 hrs by neutral red uptake assay
Cytotoxicity against human KMS11 cells after 24 hrs by neutral red uptake assay
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[PMID: 20381215] |
| LoVo | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human LoVo cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human LoVo cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| MGC-803 | IC50 |
>200 μM
Compound: UDCA
|
Anticancer activity against human MGC-803 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human MGC-803 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| RKO | IC50 |
>200 μM
Compound: UDCA
|
Anticancer activity against human RKO cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human RKO cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
| SK-HEP1 | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human SK-HEP1 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human SK-HEP1 cells assessed as inhibition of cell proliferation by MTT assay
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[PMID: 36439975] |
| SW480 | IC50 |
>200 μM
Compound: UDCA
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Anticancer activity against human SW480 cells assessed as inhibition of cell proliferation by MTT assay
Anticancer activity against human SW480 cells assessed as inhibition of cell proliferation by MTT assay
|
[PMID: 36439975] |
Ursodeoxycholic acid (10 μM; 24 h) reduces ACE2 and SHP levels in primary airway and intestinal organoids, and reduces SARS-CoV-2 infection in multiple cell types via FXR-mediated ACE2 regulation[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Ursodeoxycholic acid (1% w/w or 416 mg/kg; oral; 7 days) reduces ACE2 expression in mice and hamsters[4].
Ursodeoxycholic acid (416 mg/kg; oral; 7 days) reduces SARS-CoV-2 infection in hamsters[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5 week old C57BL/6J WT mice (male and female)[1]
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Dosage:50, 150, and 450 mg/kg dissolved in corn oil
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Administration:Oral gavage; daily for 21 days
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Result:Mice in the 50 and 450 mg/kg groups sustained significant weight loss within a week. At 50 mg/kg, this weight loss persisted over the course of the experiment. At 450 mg/kg, initially weight loss was noted during the first and third week of ursodiol administration. At 150 mg/kg, it did not have significantly different weights compared to the untreated mice.
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Animal Model:FVB/N mice and Syrian Golden Hamsters[4]
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Dosage:1% w/w for mice, 416 mg/kg for hamsters
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Administration:In chow or oral gavage, 7 days
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Result:Reduced ACE2 expression.
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Animal Model:Syrian Golden Hamsters, SARS-CoV-2 infection model[4]
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Dosage:416 mg/kg
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Administration:Oral gavage, 7 days
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Result:Prevented transmission of SARS-CoV-2 in n=6 out of 9 sentinel animals (33% infected vs. 67% uninfected).
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 128-13-2
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Appearance Solid
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분자량 392.57
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화학식 C24H40O4
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Color White to off-white
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SMILES
C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])[C@@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Synonyms
Ursodeoxycholate; Ursodiol; UDCA
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Structure Classification
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (26)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Cell Host Microbe
A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage. [Abstract]2024 Feb 14;32(2):191-208.e9. PMID: 38237593
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]
H&E-stained demonstrated Ursodeoxycholic acid (UDCA) (50 mg/kg/day, gavage, 2 weeks) and 7-keto-LCA protected against aspirin-induced intestinal epithelial damage.
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Nat Commun
2025 Aug 16;16(1):7638. PMID: 40819131
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 16;16(1):7638. [Abstract]
Mice were gavaged with B. breve or R. gnavus and then treated with BSH inhibitor riboflavin or 7β-HSDH inhibitor Ursodeoxycholic acid (UDCA) (50 mg/kg, p.o.) H&E staining showed normal, dysplastic mucosae and carcinoma in the colon tissues.
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Nat Commun
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice. [Abstract]2023 May 2;14(1):2523. PMID: 37130842
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 May 2;14(1):2523. [Abstract]
Ursodeoxycholic acid (UDCA) (0.1 mM, 6 h) blocked OA-decreased PREP activity and -increased BK content.
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Adv Sci (Weinh)
Medicago Sativa L. Saponin-Driven Lactobacillus Intestinalis Restores Intestinal Stemness in Naturally Aged Mice via the Bile Acid-FXR-Wnt Signaling Axis. [Abstract]2025 Oct 27:e15370. PMID: 41144719
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 27:e15370. [Abstract]
RT‐PCR analysis showed that Ursodeoxycholic acid (UDCA) (20 μM. 24 h) supplementation significantly increased the mRNA levels of critical markers of ISCs.
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 27:e15370. [Abstract]
Protein immunoblotting analysis showed that Ursodeoxycholic acid (UDCA) (20 μM, 24 h) supplementation reduced the protein expression of the bile acids receptor FXR.
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 27:e15370. [Abstract]
Ursodeoxycholic acid (UDCA) (20 μM, 7 days) treatment increased the protein levels of active β‐catenin in organoid cultures by immunofluorescence images.
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Adv Sci (Weinh)
Targeting FDFT1 Reduces Cholesterol and Bile Acid Production and Delays Hepatocellular Carcinoma Progression Through the HNF4A/ALDOB/AKT1 Axis. [Abstract]2025 Feb 3:e2411719. PMID: 39899681
Ursodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 3:e2411719. [Abstract]
The mRNA levels of ALDOB in Huh7 or HCCLM3 cells treated with various bile acids (100 μM, 24 h). All data are presented as mean ± SD. Data were analyzed by one-way ANOVA with Bonferroni multiple-comparison correction. CA, cholic acid; TCA, taurocholic acid; GCA, glycocholic acid; TCDCA, taurochenodeoxycholic acid; GCDCA, glycochenodeoxycholic acid; LCA, lithocholic acid; TLCA, taurolithocholic acid; GLCA, glycolithocholic acid; DCA, deoxycholic acid; TDCA, taurodeoxycholic acid; GDCA, glycodeoxycholic acid; UDCA, ursodeoxycholic acid; TUDCA, tauroursodeoxycholic acid; GUDCA, glycoursodeoxycholic acid.
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Phytomedicine
Yinzhihuang formula modulates the microbe‒gut‒liver axis and bile acid excretion to attenuate cholestatic liver injury. [Abstract]2025 Apr:139:156495. PMID: 39978276 -
Phytomedicine
Investigating the potential mechanism and therapeutic effects of SLXG for cholesterol gallstone treatment. [Abstract]2024 Sep:132:155886. PMID: 39059092 -
Free Radic Biol Med
Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2. [Abstract]2020 May 20;152:821-837. PMID: 32004633 -
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J Transl Med
Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation. [Abstract]2024 Dec 20;22(1):1124. PMID: 39707318 -
JCI Insight
FXR shapes an immunosuppressive microenvironment in PD-L1lo/- non-small cell lung cancer by upregulating HVEM. [Abstract]2025 Sep 23;10(18):e190716. PMID: 40985894 -
Cell Prolif
Suppression of the gut microbiota-bile acid-FGF19 axis in patients with atrial fibrillation. [Abstract]2023 Nov;56(11):e13488. PMID: 37186335 -
J Ethnopharmacol
Eupalinolide B ameliorates liver fibrosis by targeting PKCα to suppress the AKT/mTOR axis in hepatic stellate cells. [Abstract]2026 May 23:363:121421. PMID: 41759558 -
Cells
Cartilaginous Metabolomics Reveals the Biochemical-Niche Fate Control of Bone Marrow-Derived Stem Cells. [Abstract]2022 Sep 21;11(19):2951. PMID: 36230915 -
Commun Biol
Ursodeoxycholic acid alleviates aortic aneurysm and dissection through the intestinal farnesoid X receptor/ceramide synthase 2 axis. [Abstract]2025 Jul 5;8(1):1009. PMID: 40617906 -
Int J Mol Sci
Establishment and Validation of Novel Prognostic Subtypes in Hepatocellular Carcinoma Based on Bile Acid Metabolism Gene Signatures Using Bulk and Single-Cell RNA-Seq Data. [Abstract]2024 Jan 11;25(2):919. PMID: 38255993 -
Eur J Pharmacol
Integrative Mendelian randomization and experimental validation unveil novel druggable targets in primary biliary cholangitis. [Abstract]2026 Apr 10:1020:178775. PMID: 41819524 -
Biochim Biophys Acta Mol Basis Dis
2024 Mar;1870(3):167029. PMID: 38325224 -
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Vet Microbiol
2026 May:316:110973. PMID: 41806593 -
Neurosci Lett
Ursodeoxycholic acid protects dopaminergic neurons from oxidative stress via regulating mitochondrial function, autophagy, and apoptosis in MPTP/MPP+-induced Parkinson's disease. [Abstract]2021 Jan 10;741:135493. PMID: 33181233 -
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Clin Neuropharmacol
Protective Effects of Ursodeoxycholic Acid Against Oxidative Stress and Neuroinflammation Through Mitogen-Activated Protein Kinases Pathway in MPTP-Induced Parkinson Disease. [Abstract]2022 Nov-Dec;45(6):168-174. PMID: 36383915 -
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용액&용해도
DMSO : ≥ 100 mg/mL (254.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (280 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Winston JA, et al. Secondary bile acid ursodeoxycholic acid alters weight, the gut microbiota, and the bile acid pool in conventional mice. PLoS One. 2021;16(2):e0246161. Published 2021 Feb 18. [Content Brief]
[2]. Jackson H, et al. Influence of ursodeoxycholic acid on the mortality and malignancy associated with primary biliary cirrhosis: a population-based cohort study. Hepatology. 2007 Oct;46(4):1131-7. [Content Brief]
[3]. Kumar D, et al. Use of ursodeoxycholic acid in liver diseases. J Gastroenterol Hepatol. 2001 Jan;16(1):3-14. [Content Brief]
[4]. Brevini T, et al. FXR inhibition may protect from SARS-CoV-2 infection by reducing ACE2. Nature. 2022 Dec 5. [Content Brief]
[5]. Biao Nie, et al. Specific Bile Acids Inhibit Hepatic Fatty Acid Uptake in Mice. Hepatology. 2012 Oct;56(4):1300-10. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5473 mL | 12.7366 mL | 25.4732 mL | 63.6829 mL |
| 5 mM | 0.5095 mL | 2.5473 mL | 5.0946 mL | 12.7366 mL | |
| 10 mM | 0.2547 mL | 1.2737 mL | 2.5473 mL | 6.3683 mL | |
| 15 mM | 0.1698 mL | 0.8491 mL | 1.6982 mL | 4.2455 mL | |
| 20 mM | 0.1274 mL | 0.6368 mL | 1.2737 mL | 3.1841 mL | |
| 25 mM | 0.1019 mL | 0.5095 mL | 1.0189 mL | 2.5473 mL | |
| 30 mM | 0.0849 mL | 0.4246 mL | 0.8491 mL | 2.1228 mL | |
| 40 mM | 0.0637 mL | 0.3184 mL | 0.6368 mL | 1.5921 mL | |
| 50 mM | 0.0509 mL | 0.2547 mL | 0.5095 mL | 1.2737 mL | |
| 60 mM | 0.0425 mL | 0.2123 mL | 0.4246 mL | 1.0614 mL | |
| 80 mM | 0.0318 mL | 0.1592 mL | 0.3184 mL | 0.7960 mL | |
| 100 mM | 0.0255 mL | 0.1274 mL | 0.2547 mL | 0.6368 mL |