Lysosomal α-mannosidase
Lysosomal α-mannosidase (EC:3.2.1.24) is a low pH-activated exoglycosidase that hydrolyzes α-mannosidic linkages on mannan and N-linked oligosaccharides, and its activation requires Zn2+. As the major exoglycosidase in the glycoprotein degradation pathway, lysosomal α-mannosidase cleaves α1,2-, α1,3- and α1,6-mannosidic linkages during a non-random, ordered degradation process to generate Man1GlcNAc1-2 for further lysosomal processing. Lysosomal α-mannosidase can be used in studies related to α-mannosidosis.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Lysosomal α-mannosidase derived from rat, bovine and cat liver hydrolyzes α1,2-, α1,3- and α1,6-mannosidic linkages in N-linked glycan oligosaccharides in a non-random and species-consistent manner. It has an optimal acidic pH of 3.5-4.5, exhibits stability, and is activated by Zn2+ [2].
Bovine Lysosomal α-mannosidase is a single-chain precursor protein consisting of 949 amino acids, which can be cleaved into 5 subunits. Among these subunits, 3 are linked by disulfide bonds, and variable N-glycosylation leads to heterogeneity in its molecular mass[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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SMILES
[Lysosomal a-mannosidase]
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Synonyms
EC:3.2.1.24; MAN2B1; LAMAN
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Heikinheimo P, et al. The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation. J Mol Biol. 2003 Mar 28;327(3):631-44. [Content Brief]
[2]. Sun H, et al. Recent progress in lysosomal alpha-mannosidase and its deficiency. Experimental & molecular medicine. 2001 Mar 31;33(1):1-7. [Content Brief]
[3]. Malm D, et al. Alpha-mannosidosis. Orphanet J Rare Dis. 2008 Jul 23;3:21. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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