2. GPCR/G Protein Neuronal Signaling
  3. Dopamine Receptor
  4. Mefexamide hydrochloride

Mefexamide hydrochloride  (Synonyms: ANP-297 hydrochloride)

Cat. No.: HY-B0950A Purity: 99.45%
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Mefexamide (ANP-297) hydrochloride is a psychostimulant psychopharmaceutical and aryloxyacetic acid derivative antidepressant with dopaminergic activity. Mefexamide hydrochloride can be used for the research of depression.

For research use only. We do not sell to patients.

Mefexamide hydrochloride

Mefexamide hydrochloride Chemical Structure

CAS No. : 3413-64-7

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Based on 1 publication(s) in Google Scholar

Other Forms of Mefexamide hydrochloride:

Mefexamide hydrochloride Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Mefexamide (ANP-297) hydrochloride is a psychostimulant psychopharmaceutical and aryloxyacetic acid derivative antidepressant with dopaminergic activity. Mefexamide hydrochloride can be used for the research of depression[1][2].

In Vitro

Mefexamide (1 g; 15 min refluxed) undergoes acid hydrolysis to yield four breakdown products, and the compound exhibits distinct spectroscopic and chromatographic profiles, including a melting point of 112 °C, UV absorption maximum at 285 nm, and characteristic EI/CI mass spectra[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mefexamide hydrochloride Related Antibodies
In Vivo

Mefexamide (i.v. perfusion) hydrochloride demonstrates sympathomimetic activity by reversing Reserpine (HY-N0480)-induced ptosis in male Fauve de Bourgogne rabbits[2].
Mefexamide hydrochloride reverses Tetrabenazine (HY-B0590)-induced ptosis in male Wistar rats[2].
Mefexamide (5-20 mg/kg; i.v.; singer dose) hydrochloride significantly potentiates LSD-25-induced hyperthermia in male rabbits[2].
Mefexamide (5-20 mg/kg; i.v.; singer dose) hydrochloride potentiates Noradrenaline (HY-13715)-induced hyperthermia in male rabbits, with less significant effects than seen with LSD-25[2].
Mefexamide (10 mg/kg; i.v.; singer dose) hydrochloride protects against Reserpine (HY-N0480)-induced lowering of the bemegride convulsion threshold in male rabbits[2].
Mefexamide (10 mg/kg; i.v.; singer dose) hydrochloride does not significantly alter the EEG of normal male rabbits[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rabbit (male)[2]
Dosage: 5.0 mg/kg; 20.0 mg/kg
Administration: i.v.; singer dose
Result: Significantly potentiated LSD-25-induced hyperthermia in rabbits, mirroring the activity of cocaine.
Animal Model: Rabbit (male)[2]
Dosage: 5.0 mg/kg; 20.0 mg/kg
Administration: i.v.; singer dose
Result: Potentiated noradrenaline-induced hyperthermia in rabbits, with a similar activity profile to that observed with LSD-25, though effects were less significant.
Animal Model: Rabbit (male)[2]
Dosage: 10 mg/kg
Administration: i.v.; singer dose
Result: Reversed the reserpine-induced reduction in bemegride convulsion threshold, demonstrating antagonist activity similar to cocaine.
Animal Model: Rabbit (male)[2]
Dosage: 10 mg/kg
Administration: i.v.; singer dose
Result: Did not significantly modify the EEG of normal male Giant of Flanders rabbits, with no notable induction of large slow waves.
Molecular Weight

316.83

Formula

C15H25ClN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCN(CC)CC)COC1=CC=C(OC)C=C1.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (157.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1563 mL 15.7813 mL 31.5627 mL
5 mM 0.6313 mL 3.1563 mL 6.3125 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1563 mL 15.7813 mL 31.5627 mL 78.9067 mL
5 mM 0.6313 mL 3.1563 mL 6.3125 mL 15.7813 mL
10 mM 0.3156 mL 1.5781 mL 3.1563 mL 7.8907 mL
15 mM 0.2104 mL 1.0521 mL 2.1042 mL 5.2604 mL
20 mM 0.1578 mL 0.7891 mL 1.5781 mL 3.9453 mL
25 mM 0.1263 mL 0.6313 mL 1.2625 mL 3.1563 mL
30 mM 0.1052 mL 0.5260 mL 1.0521 mL 2.6302 mL
40 mM 0.0789 mL 0.3945 mL 0.7891 mL 1.9727 mL
50 mM 0.0631 mL 0.3156 mL 0.6313 mL 1.5781 mL
60 mM 0.0526 mL 0.2630 mL 0.5260 mL 1.3151 mL
80 mM 0.0395 mL 0.1973 mL 0.3945 mL 0.9863 mL
100 mM 0.0316 mL 0.1578 mL 0.3156 mL 0.7891 mL
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Mefexamide hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mefexamide3413-64-7ANP-297ANP297ANP 297Dopamine Receptorrabbitspsychopharmaceuticalratsdopaminergic activityhumanscatecholaminesantidepressantaryloxyacetic acid derivativemicepsychostimulantInhibitorinhibitorinhibit

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