Cat. No.: HY-10254G

Mirdametinib (GMP)

(Synonyms: PD0325901 (GMP); PD325901 (GMP))

1 Cited Publications

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Mirdametinib (PD0325901) (GMP) is Mirdametinib (HY-10254) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Mirdametinib is an orally active, selective and non-ATP-competitive MEK inhibitor.

For research use only. We do not sell to patients.

CAS No.: 391210-10-9
Formula: C₁₆H₁₄F₃IN₂O₄
Molecular Weight:482.19

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Mirdametinib (GMP) Chemical Structure

CAS No.: 391210-10-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Purity & Documentation
References
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Biological Activity

Description

All MEK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	13 nM Compound: PD325901	Antiproliferative activity against human A375 cells expressing BRAF V600E mutant after 72 hrs by Cell titer-glo assay Antiproliferative activity against human A375 cells expressing BRAF V600E mutant after 72 hrs by Cell titer-glo assay	[PMID: 23474388]
A-375	GI₅₀	1.28 nM Compound: 5; PD-325901	Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition incubated for 72 hrs in the absence of UV irradiation by CellTiter-Glo luminescent assay Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition incubated for 72 hrs in the absence of UV irradiation by CellTiter-Glo luminescent assay	[PMID: 36924668]
A-375	GI₅₀	1.37 nM Compound: 5; PD-325901	Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 1 min and measured 72 hrs post compound treatment by CellTiter-Glo lumi Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 1 min and measured 72 hrs post compound treatment by CellTiter-Glo lumi	[PMID: 36924668]
A-375	GI₅₀	1.49 nM Compound: 5; PD-325901	Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 5 mins and measured 72 hrs post compound treatment by CellTiter-Glo lum Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 5 mins and measured 72 hrs post compound treatment by CellTiter-Glo lum	[PMID: 36924668]
A-375	GI₅₀	1.59 nM Compound: 5; PD-325901	Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 3 mins and measured 72 hrs post compound treatment by CellTiter-Glo lum Antiproliferative activity against human A-375 cells harboring BRAF V600E mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 3 mins and measured 72 hrs post compound treatment by CellTiter-Glo lum	[PMID: 36924668]
A-431	IC₅₀	<0.014 μM Compound: PD-0325901	Inhibition of MEK/ERK signaling pathway in TNFalpha-stimulated human A431 cells transfected with COT siRNA assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by stimulation with TNFalpha for 10 mins by Western blot analysis Inhibition of MEK/ERK signaling pathway in TNFalpha-stimulated human A431 cells transfected with COT siRNA assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by stimulation with TNFalpha for 10 mins by Western blot analysis	[PMID: 27502541]
A-431	IC₅₀	0.038 μM Compound: PD-0325901	Inhibition of MEK/ERK signaling pathway in EGF-stimulated human A431 cells transfected with COT siRNA assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by stimulation with EGF for 10 mins by Western blot analysis Inhibition of MEK/ERK signaling pathway in EGF-stimulated human A431 cells transfected with COT siRNA assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by stimulation with EGF for 10 mins by Western blot analysis	[PMID: 27502541]
A549	IC₅₀	166 nM Compound: PD325901	Antiproliferative activity against human A549 cells Antiproliferative activity against human A549 cells	[PMID: 23474388]
A549	IC₅₀	166 nM Compound: PD325901	Antiproliferative activity against human A549 cells harboring K-Ras mutation Antiproliferative activity against human A549 cells harboring K-Ras mutation	[PMID: 26611920]
A549	IC₅₀	0.06 μM Compound: PD0325901	Antiproliferative activity against human A549 cells harboring KRAS G12S mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human A549 cells harboring KRAS G12S mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
A549	CC₅₀	>100 μM Compound: PD-0325901	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 33539089]
A549	GI₅₀	10.6 nM Compound: 5; PD-325901	Antiproliferative activity against human A549 cells harboring KRas G13S mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 5 mins and measured 72 hrs post compound treatment by CellTiter-Glo lumin Antiproliferative activity against human A549 cells harboring KRas G13S mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 5 mins and measured 72 hrs post compound treatment by CellTiter-Glo lumin	[PMID: 36924668]
A549	GI₅₀	4.94 nM Compound: 5; PD-325901	Antiproliferative activity against human A549 cells harboring KRAS G13S mutant assessed as cell growth inhibition incubated for 72 hrs in the absence of UV irradiation by CellTiter-Glo luminescent assay Antiproliferative activity against human A549 cells harboring KRAS G13S mutant assessed as cell growth inhibition incubated for 72 hrs in the absence of UV irradiation by CellTiter-Glo luminescent assay	[PMID: 36924668]
COLO 205	IC₅₀	5 nM Compound: PD325901	Antiproliferative activity against human COLO205 cells harboring BRAF mutation Antiproliferative activity against human COLO205 cells harboring BRAF mutation	[PMID: 26611920]
COLO 205	GI₅₀	0.2 nM Compound: 4; PD0325901	Antiproliferative activity against human COLO205 cells assessed as reduction in cell growth after 3 days by WST8 assay Antiproliferative activity against human COLO205 cells assessed as reduction in cell growth after 3 days by WST8 assay	[PMID: 31730343]
HCT-116	IC₅₀	194 nM Compound: PD325901	Antiproliferative activity against human HCT116 cells expressing KRAS G13D mutant Antiproliferative activity against human HCT116 cells expressing KRAS G13D mutant	[PMID: 23474388]
HCT-116	IC₅₀	185 nM Compound: PD325901	Antiproliferative activity against human HCT116 cells harboring K-Ras G13D mutation Antiproliferative activity against human HCT116 cells harboring K-Ras G13D mutation	[PMID: 26611920]
HCT-116	IC₅₀	0.09 μM Compound: PD0325901	Antiproliferative activity against human HCT116 cells harboring KRAS G13D mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human HCT116 cells harboring KRAS G13D mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
HCT-116	GI₅₀	44.7 nM Compound: 5; PD-325901	Antiproliferative activity against human HCT-116 cells harboring KRAS G13D mutant assessed as cell growth inhibition incubated for 72 hrs in the absence of UV irradiation by CellTiter-Glo luminescent assay Antiproliferative activity against human HCT-116 cells harboring KRAS G13D mutant assessed as cell growth inhibition incubated for 72 hrs in the absence of UV irradiation by CellTiter-Glo luminescent assay	[PMID: 36924668]
HCT-116	GI₅₀	52.5 nM Compound: 5; PD-325901	Antiproliferative activity against human HCT-116 cells harboring KRAS G13D mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 5 mins and measured 72 hrs post compound treatment by CellTiter-Glo lu Antiproliferative activity against human HCT-116 cells harboring KRAS G13D mutant assessed as cell growth inhibition preincubated with compound for 1 hrs followed by UV irradiation for 5 mins and measured 72 hrs post compound treatment by CellTiter-Glo lu	[PMID: 36924668]
HEK-293T	GI₅₀	>10000 nM Compound: 5; PD-325901	Cytotoxicity against HEK293T cells assessed as cell growth inhibition incubated for 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity against HEK293T cells assessed as cell growth inhibition incubated for 72 hrs by CellTiter-Glo luminescent assay	[PMID: 36924668]
HepG2	IC₅₀	5 nM Compound: PD325901	Antiproliferative activity against human HepG2 cells harboring N-Ras mutation Antiproliferative activity against human HepG2 cells harboring N-Ras mutation	[PMID: 26611920]
HL-60	IC₅₀	0.0013 μM Compound: PD0325901	Antiproliferative activity against human HL60 cells harboring NRAS K61L mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human HL60 cells harboring NRAS K61L mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
HT-29	GI₅₀	3.1 nM Compound: 4; PD0325901	Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth after 3 days by MTT assay Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth after 3 days by MTT assay	[PMID: 31730343]
LoVo	IC₅₀	128 nM Compound: PD325901	Antiproliferative activity against human LoVo cells Antiproliferative activity against human LoVo cells	[PMID: 23474388]
MCF7	IC₅₀	9550 nM Compound: PD325901	Antiproliferative activity against human MCF7 cells Antiproliferative activity against human MCF7 cells	[PMID: 26611920]
MDA-MB-231	GI₅₀	1 μM Compound: PD-0325901	Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by CellTiter-Glo luminescence assay Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by CellTiter-Glo luminescence assay	[PMID: 30802730]
MDA-MB-231	IC₅₀	0.082 μM Compound: PD0325901	Antiproliferative activity against human MDA-MB-231 cells harboring KRAS G13D/BRAF G464V mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human MDA-MB-231 cells harboring KRAS G13D/BRAF G464V mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
MIA PaCa-2	IC₅₀	17 nM Compound: PD325901	Antiproliferative activity against human MIAPaCa2 cells Antiproliferative activity against human MIAPaCa2 cells	[PMID: 23474388]
Sf21	IC₅₀	>25 μM Compound: PD-0325901	Inhibition of human COT (66 to 395 residues) expressed in Sf21 cells using 5-Fluo-Ahx-AGAGSGQLIDSNleANSFVGTR-NH2 as substrate after 60 mins by caliper microfluidic mobility shift assay Inhibition of human COT (66 to 395 residues) expressed in Sf21 cells using 5-Fluo-Ahx-AGAGSGQLIDSNleANSFVGTR-NH2 as substrate after 60 mins by caliper microfluidic mobility shift assay	[PMID: 27502541]
SK-MEL-28	GI₅₀	2.9 nM Compound: 4; PD0325901	Antiproliferative activity against human SK-MEL-28 cells assessed as reduction in cell growth after 3 days by MTT assay Antiproliferative activity against human SK-MEL-28 cells assessed as reduction in cell growth after 3 days by MTT assay	[PMID: 31730343]
SU-DHL-1	GI₅₀	350 nM Compound: 1; PD0325901	Antiproliferative activity against human SU-DHL-1 cells incubated for 3 days by WST8 assay Antiproliferative activity against human SU-DHL-1 cells incubated for 3 days by WST8 assay	[PMID: 33284613]
UACC-257	GI₅₀	0.2 nM Compound: 4; PD0325901	Antiproliferative activity against human UACC257 cells assessed as reduction in cell growth after 3 days by MTT assay Antiproliferative activity against human UACC257 cells assessed as reduction in cell growth after 3 days by MTT assay	[PMID: 31730343]

In Vitro

Mirdametinib (250 nM; 7 d) induces differentiation of cardiac PCs into vascular smooth muscle cell (VSMC)-like cells^[1].
Mirdametinib (10 μM; 4 d) induces oligodendrocyte precursor cells (OPC) to oligodendrocytes (OL) differentiation^[2].
Mirdametinib enhances embryonic stem cells (ESC) self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 391210-10-9
Appearance Solid
Molecular Weight 482.19
Formula C₁₆H₁₄F₃IN₂O₄
Color White to off-white
SMILES

O=C(C1=CC=C(C(F)=C1NC2=CC=C(I)C=C2F)F)NOC[C@H](O)CO
Synonyms

PD0325901 (GMP); PD325901 (GMP)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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bioRxiv

2025 Nov 12.

Purity & Documentation

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Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Avolio E, et al. Cardiac pericyte reprogramming by MEK inhibition promotes arteriologenesis and angiogenesis of the ischemic heart. J Clin Invest. 2022 May 16;132(10):e152308. [Content Brief]

[2]. Suo N, et al. Inhibition of MAPK/ERK pathway promotes oligodendrocytes generation and recovery of demyelinating diseases. Glia. 2019 Jul;67(7):1320-1332. [Content Brief]

[3]. Ai Z, et al. Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901. Stem Cells Int. 2016;2016:1792573. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Mirdametinib (GMP)

1 Cited Publications

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Mirdametinib (GMP)

Publications Citing Use of MedChemExpress (MCE) Mirdametinib (GMP)

All MEK Isoforms

Biological Activity

Chemical Information

Publications (1)

Purity & Documentation

References

Calculators

Help & FAQs

Mirdametinib (GMP) Related Classifications

HY-10254G Related Classifications