Nourseothricin sulfate
Based on 2 publication(s) in Google Scholar
Nourseothricin sulfate (Streptothricin sulfate) is a broad-spectrum antibiotic that destroys the outer membrane of Gram-negative bacteria and is a dominant selective marker for Fonsecaea pedrosoi. Nourseothricin sulfate inhibits protein biosynthesis in prokaryotic cells and strongly inhibits the growth of eukaryotes like fungi and can also be used as a elective marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells.
For research use only. We do not sell to patients.
- Purity: 99.9%
- CAS No.: 96736-11-7
- Formula: C50H94N20O22S
- Molecular Weight:1359.47
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Nourseothricin sulfate
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Microbiological Assay
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Microbiological Assay
All Antibiotic Isoforms
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Biological Activity
Escherichia coli resistance to neurosporin can be sensitive to resistant strains by eliminating their outer membrane resistance. The polycationic antibiotic, Nourseothricin, represents a mixture of several Streptothricins, mainly D and F. Obviously, although very slowly, it can pass the outer membrane via the porin pores. It has been shown earlier that Nourseothricin is able to generate some kind of channels into the outer membrane through which it can pass the cell wall. On the other hand, there are indications that resistant strains containing a Nourseothricin-inactivating acetyl transferase possess an additional protecting system, namely a reduced penetrability of the outer membrane[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 96736-11-7
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Appearance Solid
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Molecular Weight 1359.47
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Formula C50H94N20O22S
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Color White to off-white
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SMILES
NC(O[C@H]1[C@@H](CO)O[C@@H](NC(N2)=N[C@]([C@@]2([H])[C@H](O)CN3)([H])C3=O)[C@H](NC(C[C@@H](N)CCCN(C(C[C@@H](N)CCCNC(C[C@@H](N)CCCN)=O)=O)[H])=O)[C@@H]1O)=O.O=S(O)(O)=O.NC(O[C@H]4[C@@H](CO)O[C@@H](NC(N5)=N[C@]([C@@]5([H])[C@H](O)CN6)([H])C6=O)[C@H](NC(C[C@@H](N)CCCN)=O)[C@@H]4O)=O
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Synonyms
Streptothricin sulfate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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BMC Microbiol
P4-ATPase subunit Cdc50 plays a role in yeast budding and cell wall integrity in Candida glabrata. [Abstract]2023 Apr 13;23(1):99. PMID: 37046215 -
Microbiol Spectr
Nourseothricin as a novel therapeutic agent against Neisseria gonorrhoeae: in vitro and in vivo evaluations using Galleria mellonella-a pilot study. [Abstract]2025 Aug 5;13(8):e0127525. PMID: 40662736
Nourseothricin sulfate purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2025 Aug 5;13(8):e0127525. [Abstract]
Survival curve of the antimicrobial therapeutic group. Survival curve of Galleria mellonella infected with N. gonorrhoeae (6 McF) and treated with varying doses of Nourseothricin (0-128 μg/mL, 0-5 days), Ceftriaxone, or PBS.
Nourseothricin sulfate purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2025 Aug 5;13(8):e0127525. [Abstract]
Toxicity of nourseothricin. To assess nourseothricin toxicity, the impact of different concentrations of Nourseothricin (0-128 µg/mL, 0-5 days) on larval survival was compared with that of larvae that received no injection and larvae injected with 30 µL of PBS. Survival proportions for these groups were recorded over 4 days, and the probability of survival was analyzed accordingly. NTC, nourseothricin; PBS, phosphate-buffered saline.
Solvent & Solubility
H2O : ≥ 100 mg/mL (73.56 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (73.56 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (288 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Seltmann G. et al. Resistance of Escherichia coli to nourseothricin (streptothricin): sensitization of resistant strains by abolition of its outer membrane resistance. Zentralbl Bakteriol. 1992 Jan;276(2):143-51. [Content Brief]
[2]. Florencio CS, et al. Genetic manipulation of Fonsecaea pedrosoi using particles bombardment and Agrobacterium mediated transformation. Microbiol Res. 2018 Mar;207:269-279. [Content Brief]
[3]. Suárez-Moreno ZR, et al. Plant-Growth Promotion and Biocontrol Properties of Three Streptomyces spp. Isolates to Control Bacterial Rice Pathogens.. Front Microbiol. 2019 Feb 25;10:290. [Content Brief]
[4]. Bräunlich H, et al. Renal handling of nourseothricin. Pharmazie. 1988 Mar;43(3):200-2. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.7356 mL | 3.6779 mL | 7.3558 mL | 18.3895 mL |
| 5 mM | 0.1471 mL | 0.7356 mL | 1.4712 mL | 3.6779 mL | |
| 10 mM | 0.0736 mL | 0.3678 mL | 0.7356 mL | 1.8390 mL | |
| 15 mM | 0.0490 mL | 0.2452 mL | 0.4904 mL | 1.2260 mL | |
| 20 mM | 0.0368 mL | 0.1839 mL | 0.3678 mL | 0.9195 mL | |
| 25 mM | 0.0294 mL | 0.1471 mL | 0.2942 mL | 0.7356 mL | |
| 30 mM | 0.0245 mL | 0.1226 mL | 0.2452 mL | 0.6130 mL | |
| 40 mM | 0.0184 mL | 0.0919 mL | 0.1839 mL | 0.4597 mL | |
| 50 mM | 0.0147 mL | 0.0736 mL | 0.1471 mL | 0.3678 mL | |
| 60 mM | 0.0123 mL | 0.0613 mL | 0.1226 mL | 0.3065 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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