2. Anti-infection
  3. Fungal Bacterial Antibiotic
  4. Nourseothricin sulfate

Nourseothricin sulfate  (Synonyms: Streptothricin sulfate)

Cat. No.: HY-129065 Purity: 99.9%
COA
SDS
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Nourseothricin sulfate (Streptothricin sulfate) is a broad-spectrum antibiotic that destroys the outer membrane of Gram-negative bacteria and is a dominant selective marker for Fonsecaea pedrosoi. Nourseothricin sulfate inhibits protein biosynthesis in prokaryotic cells and strongly inhibits the growth of eukaryotes like fungi and can also be used as a elective marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells.

For research use only. We do not sell to patients.

CAS No. : 96736-11-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Based on 2 publication(s) in Google Scholar

Nourseothricin sulfate Related Antibodies

Top Publications Citing Use of Products
Microbiological Assay

    Nourseothricin sulfate purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2025 Aug 5;13(8):e0127525.  [Abstract]

    Survival curve of the antimicrobial therapeutic group. Survival curve of Galleria mellonella infected with N. gonorrhoeae (6 McF) and treated with varying doses of Nourseothricin (0-128 μg/mL, 0-5 days), Ceftriaxone, or PBS.

    Nourseothricin sulfate purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2025 Aug 5;13(8):e0127525.  [Abstract]

    Toxicity of nourseothricin. To assess nourseothricin toxicity, the impact of different concentrations of Nourseothricin (0-128 µg/mL, 0-5 days) on larval survival was compared with that of larvae that received no injection and larvae injected with 30 µL of PBS. Survival proportions for these groups were recorded over 4 days, and the probability of survival was analyzed accordingly. NTC, nourseothricin; PBS, phosphate-buffered saline.

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    • Biological Activity

    • Purity & Documentation

    • References

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    Description

    Nourseothricin sulfate (Streptothricin sulfate) is a broad-spectrum antibiotic that destroys the outer membrane of Gram-negative bacteria and is a dominant selective marker for Fonsecaea pedrosoi[1][2]. Nourseothricin sulfate inhibits protein biosynthesis in prokaryotic cells and strongly inhibits the growth of eukaryotes like fungi and can also be used as a elective marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells[3].

    In Vitro

    Escherichia coli resistance to neurosporin can be sensitive to resistant strains by eliminating their outer membrane resistance. The polycationic antibiotic, Nourseothricin, represents a mixture of several Streptothricins, mainly D and F. Obviously, although very slowly, it can pass the outer membrane via the porin pores. It has been shown earlier that Nourseothricin is able to generate some kind of channels into the outer membrane through which it can pass the cell wall. On the other hand, there are indications that resistant strains containing a Nourseothricin-inactivating acetyl transferase possess an additional protecting system, namely a reduced penetrability of the outer membrane[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nourseothricin sulfate Related Antibodies
    In Vivo

    Nourseothricin is preferentially excreted via kidney and signs of nephrotoxicity can be observed after its administration. Renal handling of Nourseothricin is characterized in experiments on renal cortical slices under various experimental conditions. Following administration in vivo the renal tubular transport system for organic anions (p-aminohippurate, PAH) is not influenced by Nourseothricin. There is a high degree of accumulation of Nourseothricin in renal cortical slices. In contrast to PAH accumulation there is no influence of nitrogen atmosphere, simultaneous administration of PAH, probenecid or trishydroxyaminomethane on Nourseothricin accumulation. Age dependent differences in Nourseothricin accumulation does not exist[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    1359.47

    Formula

    C50H94N20O22S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(O[C@H]1[C@@H](CO)O[C@@H](NC(N2)=N[C@]([C@@]2([H])[C@H](O)CN3)([H])C3=O)[C@H](NC(C[C@@H](N)CCCN(C(C[C@@H](N)CCCNC(C[C@@H](N)CCCN)=O)=O)[H])=O)[C@@H]1O)=O.O=S(O)(O)=O.NC(O[C@H]4[C@@H](CO)O[C@@H](NC(N5)=N[C@]([C@@]5([H])[C@H](O)CN6)([H])C6=O)[C@H](NC(C[C@@H](N)CCCN)=O)[C@@H]4O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (73.56 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7356 mL 3.6779 mL 7.3558 mL
    5 mM 0.1471 mL 0.7356 mL 1.4712 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (73.56 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.9%

    SDS (396 KB)

    COA (254 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.7356 mL 3.6779 mL 7.3558 mL 18.3895 mL
    5 mM 0.1471 mL 0.7356 mL 1.4712 mL 3.6779 mL
    10 mM 0.0736 mL 0.3678 mL 0.7356 mL 1.8390 mL
    15 mM 0.0490 mL 0.2452 mL 0.4904 mL 1.2260 mL
    20 mM 0.0368 mL 0.1839 mL 0.3678 mL 0.9195 mL
    25 mM 0.0294 mL 0.1471 mL 0.2942 mL 0.7356 mL
    30 mM 0.0245 mL 0.1226 mL 0.2452 mL 0.6130 mL
    40 mM 0.0184 mL 0.0919 mL 0.1839 mL 0.4597 mL
    50 mM 0.0147 mL 0.0736 mL 0.1471 mL 0.3678 mL
    60 mM 0.0123 mL 0.0613 mL 0.1226 mL 0.3065 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Nourseothricin sulfate Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nourseothricin96736-11-7StreptothricinFungalBacterialAntibioticInhibitorinhibitorinhibit

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