Pinobanksin 3-acetate
Based on 1 Customer Validation
Pinobanksin 3-acetate is a flavanone and flavonoid ester with antioxidant activity. Pinobanksin 3-acetate is naturally present in Sonoran propolis, honey, medicinal plants, and forestry coproducts of Pinus caribaea. Pinobanksin 3-acetate exerts anti-colorectal cancer activity by modulating proliferation, apoptosis, cell cycle, and Wnt signaling pathways. Pinobanksin 3-acetate can be used for the research of colon cancer.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 52117-69-8
- Formula: C17H14O6
- Molecular Weight:314.29
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Pinobanksin 3-acetate does not inhibit proliferation of murine B-cell lymphoma M12.C3.F6 cells in an MTT assay and does not induce apoptosis in these cells[1].
Pinobanksin 3-acetate does not inhibit the growth of Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, or Candida albicans[2].
Pinobanksin 3-acetate (5-150 μg/mL; 24-72 h) potently inhibits proliferation of human colon cancer SW480 cells in a time- and dose-dependent manner, with IC50 values of 163.61 μg/mL at 24 h, 40.72 μg/mL at 48 h, and 45.56 μg/mL at 72 h[3].
Pinobanksin 3-acetate (50, 100 μg/mL; 24 h) dose-dependently increases the apoptotic rate of human colon cancer SW480 cells[3].
Pinobanksin 3-acetate (100 μg/mL; 24 h) alters the expression of 4912 genes in human colon cancer SW480 cells, with differential expression enriched in pathways linked to cell proliferation, apoptosis, and colorectal carcinogenesis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:murine B-cell lymphoma M12.C3.F6 cells
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Concentration:50 μM
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Incubation Time:12 h
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Result:Did not exhibit a pro-apoptotic effect in M12.C3.F6 cells, with no statistical difference from the DMSO vehicle control.
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Cell Line:human colon cancer SW480 cells
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Concentration:5 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 150 μg/mL
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Incubation Time:24 h, 48 h, 72 h
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Result:Exerted a significant inhibitory effect on SW480 cell proliferation in a time- and dose-dependent manner.
Exhibited IC50 values of 163.61 μg/mL at 24 h, 40.72 μg/mL at 48 h, and 45.56 μg/mL at 72 h.
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Cell Line:human colon cancer SW480 cells
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Concentration:50 μg/mL, 100 μg/mL
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Incubation Time:24 h
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Result:Induced early apoptosis in 32.50% of SW480 cells and late apoptosis in 9.55% of cells at 50 μg/mL after 24 h treatment.
Induced early apoptosis in 49.60% of cells and late apoptosis in 24.10% of cells at 100 μg/mL after 24 h treatment.
Increased the apoptotic rate in a dose-dependent manner.
Chemical Information
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CAS No. 52117-69-8
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Appearance Solid
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Molecular Weight 314.29
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Formula C17H14O6
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Color White to off-white
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SMILES
O=C1[C@H](OC(C)=O)[C@@H](C2=CC=CC=C2)OC3=CC(O)=CC(O)=C13
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (318.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1818 mL | 15.9089 mL | 31.8177 mL | 79.5444 mL |
| 5 mM | 0.6364 mL | 3.1818 mL | 6.3635 mL | 15.9089 mL | |
| 10 mM | 0.3182 mL | 1.5909 mL | 3.1818 mL | 7.9544 mL | |
| 15 mM | 0.2121 mL | 1.0606 mL | 2.1212 mL | 5.3030 mL | |
| 20 mM | 0.1591 mL | 0.7954 mL | 1.5909 mL | 3.9772 mL | |
| 25 mM | 0.1273 mL | 0.6364 mL | 1.2727 mL | 3.1818 mL | |
| 30 mM | 0.1061 mL | 0.5303 mL | 1.0606 mL | 2.6515 mL | |
| 40 mM | 0.0795 mL | 0.3977 mL | 0.7954 mL | 1.9886 mL | |
| 50 mM | 0.0636 mL | 0.3182 mL | 0.6364 mL | 1.5909 mL | |
| 60 mM | 0.0530 mL | 0.2651 mL | 0.5303 mL | 1.3257 mL | |
| 80 mM | 0.0398 mL | 0.1989 mL | 0.3977 mL | 0.9943 mL | |
| 100 mM | 0.0318 mL | 0.1591 mL | 0.3182 mL | 0.7954 mL |
Pinobanksin 3-acetate Related Classifications
Cat. No.: HY-N1854
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- Pinobanksin 3-acetate
- 52117-69-8
- Drug Derivative
- Apoptosis
- Wnt
- Calcium signaling pathways
- B-cell lymphoma cells
- Pinus caribaea
- human colon cancer SW480 cells
- colorectal carcinogenesis
- Wnt signaling pathways
- apoptosis
- Staphylococcus epidermidis
- Sonoran propolis
- murine B-cell lymphoma M12.C3.F6 cells
- Inhibitor
- inhibitor
- inhibit