The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the Hotdog-Fold family

  • Biochemistry. 2009 Jun 23;48(24):5507-9. doi: 10.1021/bi900710w.
Hong Zhao  1 ,  Brian M Martin ,  Marco Bisoffi ,  Debra Dunaway-Mariano
Affiliations
  • 1. Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA.
PMID: 19453107 DOI: 10.1021/bi900710w
Abstract

Herein, we report on an in vitro kinetic activity analysis that demonstrates that the protein known as the Akt C-terminal modulator protein is a broad-range, high-activity acyl-CoA thioesterase. In vitro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative results. Truncation mutants confined the thioesterase activity to the C-terminal domain, consistent with our threading model. The N-terminal domain of unknown fold and function was found to contribute to solubility.