Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells
- Tuberc Respir Dis (Seoul). 2014 Nov;77(5):203-8. doi: 10.4046/trd.2014.77.5.203.
- 1. Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
- 2. Department of Pharmacy, College of Pharmacy, Yeungnam University, Daegu, Korea.
- 3. Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul, Korea.
- 4. Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Daejeon, Korea.
Background: In this study, we investigated whether lobetyolin, lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells.
Methods: Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and Mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.
Results: Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5AC mucin production. However, lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion.
Conclusion: These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells.
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Cat. No.Product NameDescriptionTargetResearch Area
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Research Areas: Metabolic Disease