2. Apoptosis Metabolic Enzyme/Protease
  3. Apoptosis Xanthine Oxidase
  4. Lobetyolin

Lobetyolin 

Cat. No.: HY-N0327 Purity: 99.89%
COA Handling Instructions

Lobetyolin, a bioactive compound, is derived from Codonopsis pilosula. Lobetyolin has anti-inflammatory, anti-oxidative and xanthine oxidase inhibiting activities. Lobetyolin also induces the apoptosis via the inhibition of ASCT2-mediated glutamine metabolism.

For research use only. We do not sell to patients.

Lobetyolin Chemical Structure

Lobetyolin Chemical Structure

CAS No. : 129277-38-9

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Lobetyolin Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Lobetyolin, a bioactive compound, is derived from Codonopsis pilosula. Lobetyolin has anti-inflammatory, anti-oxidative and xanthine oxidase inhibiting activities. Lobetyolin also induces the apoptosis via the inhibition of ASCT2-mediated glutamine metabolism[1][2].

In Vitro

Lobetyolin is derived from Codonopsis pilosula and has antioxidative effect. MUC5AC gene expression induced by phorbol 12-myristate 13-acetate (PMA) from NCI-H292 cells is inhibited by pretreatment with Lobetyolin, lobetyol or methyl linoleate. Lobetyolin does not affect PMA-induced MUC5AC production from NCI-H292 cells. The amounts of mucin in the cells of Lobetyolin-treated cultures are 100±25%, 487±33%, 524±38%, 411±24%, and 402±45% for control, 10 ng/mL of PMA alone, PMA plus Lobetyolin 1 μM, PMA plus Lobetyolin 10 μM, and PMA plus Lobetyolin 100 μM, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Lobetyolin Related Antibodies
Molecular Weight

396.43

Appearance

Solid

Formula

C20H28O8
CAS No.
SMILES

O[C@H](C#CC#C/C=C/C)[C@@H](/C=C/CCCO)O[C@@H]([C@@H]([C@H]1O)O)O[C@@H]([C@H]1O)CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (126.13 mM; Need ultrasonic)

H2O : 50 mg/mL (126.13 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5225 mL 12.6126 mL 25.2251 mL
5 mM 0.5045 mL 2.5225 mL 5.0450 mL
10 mM 0.2523 mL 1.2613 mL 2.5225 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 33.33 mg/mL (84.08 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.31 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.31 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.31 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.89%

COA (248 KB) HNMR (293 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

After 24 hours of serum deprivation, cells are pretreated with Lobetyolin, lobetyol or methyl linoleate (1, 10, and 100 µM), for 30 minutes and then treated with phorbol 12-myristate 13-acetate (PMA; 10 ng/mL) for 24 hours in serum-free RPMI 1640. After 24 hours, the spent media are collected to measure the secretion of MUC5AC protein and cells are lysed with buffer solution containing 20 mM Tris, 0.5% NP-40, 250 mM NaCl, 3 mM EDTA, 3 mM EGTA, and protease inhibitor cocktail and collected to measure the production of MUC5AC protein (in 24-well culture plate)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Lobetyolin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Keywords:

Lobetyolin129277-38-9ApoptosisXanthine OxidaseXOCodonopsispilosulaanti-inflammatoryanti-oxidativexanthineoxidaseapoptosisASCT2Inhibitorinhibitorinhibit

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