Atractylenolide III (Synonyms: ICodonolactone; 8β-Hydroxyasterolide)

Name: Atractylenolide III
Brand: MedChemExpress
SKU: HY-N0203
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0203 Purity: 99.91%: Data Sheet
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Atractylenolide III (ICodonolactone) is the main component of Atractylodes rhizome and has the activity of inducing apoptosis in lung cancer cells. Atractylenolide III is an orally active gastroprotective agent.

For research use only. We do not sell to patients.

CAS No. : 73030-71-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
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Based on 3 publication(s) in Google Scholar

Other Forms of Atractylenolide III:

Atractylenolide III (Standard) In-stock

3 Publications Citing Use of MCE Atractylenolide III

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Histological Imaging/Staining

: Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]; Protein expression of ASCT2 in atractylenolide III (0, 1, 2, 5, 10, 20, 40, 80, 100, 200 μM) concentration gradient-treated HSC-LX2 cells by Western blot.

: Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]; Intracellular glutamine, glutamate, α-KG, GSH and ATP levels were evaluated by kits in atractylenolide III (40 μM)-treated HSC-LX2 cells.

: Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]; Cell viability was tested in re-expression ASCT2-WT or ASCT2-N230G into atractylenolide III-treatment LX2 cells.

: Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]; Representative images of SA-β-Gal staining and α-SMA IHC in cryosections from mouse liver sections treated with Atractylenolide III (40 mg/kg).

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Biological Activity
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	100.7 μM Compound: 1	Inhibition of human SOAT2-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis Inhibition of human SOAT2-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis	[PMID: 32035699]
CHO	IC₅₀	174.9 μM Compound: 1	Inhibition of human SOAT1 mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis Inhibition of human SOAT1 mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis	[PMID: 32035699]
CHO	IC₅₀	187.3 μM Compound: 1	Inhibition of human SOAT2-mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis Inhibition of human SOAT2-mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis	[PMID: 32035699]
CHO	IC₅₀	55.1 μM Compound: 1	Inhibition of human SOAT1-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis Inhibition of human SOAT1-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis	[PMID: 32035699]
CHO-K1	IC₅₀	>195 μM Compound: 1	Inhibition of [14C]triglyceride biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method Inhibition of [14C]triglyceride biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method	[PMID: 32035699]
CHO-K1	IC₅₀	>195 μM Compound: 1	Cytotoxicity against CHOK1 cells assessed as reduction in cell viability Cytotoxicity against CHOK1 cells assessed as reduction in cell viability	[PMID: 32035699]
CHO-K1	IC₅₀	211 μM Compound: 1	Inhibition of SOAT in CHOK1 microsomes Inhibition of SOAT in CHOK1 microsomes	[PMID: 32035699]
CHO-K1	IC₅₀	73.5 μM Compound: 1	Inhibition of [14C]cholesterol ester biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method Inhibition of [14C]cholesterol ester biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method	[PMID: 32035699]
PC-12	EC₅₀	36.3 μM Compound: 16; 19	Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay	[PMID: 29775304]
RAW264.7	IC₅₀	>100 μM Compound: 8	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 24 hrs in presence of LPS by colorimetric analysis Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 24 hrs in presence of LPS by colorimetric analysis	[PMID: 27865705]

In Vitro

Atractylenolide III (1-100 μM, 48 h ) induces cell apoptosis, and induces the activation of caspase-3 and caspase-9 and cleavage of PARP in A549 cells^[1].
Atractylenolide III (1-100 μM, 72 h) inhibits proliferation and angiogenesis (tube formation) in HUVECs^[1].
Atractylenolide III (1-100 μM) inhibits thymic stromal lymphopoietin (TSLP)-induced production of proinflammatory cytokines (IL-6, IL-1b, TNF-α, and IL-8) in HMC-1 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	A549
Concentration:	1, 10, 100 μM
Incubation Time:	24 h, 48 h
Result:	Increased active form of caspase-9, caspase-3, and the cleavage of PARP at 48 h. Increased the expression of proapoptotic protein bax, and induced AIF translocation to the nucleus at 24 h.

In Vivo

Atractylenolide III (5 and 10 mg/kg, p.o.) shows gastroprotective effects and reduces 70% ethanol-induced gastric ulcer in rats^[2].
Atractylenolide III (30 mg/kg, oral gavage for 14 days or 28 days) reduces depressive- and anxiogenic-like behaviors in rat depression models in Lipopolysaccharide (LPS) (HY-D1056) and chronic unpredictable mild stress (CUMS) induced rat depression model^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	70% ethanol-induced gastric ulcers in rats^[2]
Dosage:	5 and 10 mg/kg
Administration:	p.o.
Result:	Inhibited gastric ulcer formation and the necrotic erosion of gastric mucosa. Downregulated the MMP-2/9 expression by activating the TIMP-2 and TIMP-1 expressions.

Molecular Weight

248.32

Formula

C₁₅H₂₀O₃

CAS No.

73030-71-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C(C)=C(C[C@@]23[H])[C@@](C[C@@]3(C)CCCC2=C)(O)O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (201.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0271 mL	20.1353 mL	40.2706 mL
5 mM	0.8054 mL	4.0271 mL	8.0541 mL
10 mM	0.4027 mL	2.0135 mL	4.0271 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (252 KB) HNMR (219 KB) LCMS (115 KB)

Handling Instructions (2659 KB)

References

[1]. Kang TH, et al. Atractylenolide III, a sesquiterpenoid, induces apoptosis in human lung carcinoma A549 cells via mitochondria-mediated death pathway. Food Chem Toxicol. 2011 Feb;49(2):514-9. [Content Brief]

[2]. Wang KT, et al. Gastroprotective activity of atractylenolide III from Atractylodes ovata on ethanol-induced gastric ulcer in vitro and in vivo. J Pharm Pharmacol. 2010 Mar;62(3):381-8. [Content Brief]

[3]. Kim HK, et al. Toxicity of atractylon and atractylenolide III Identified in Atractylodes ovata rhizome to Dermatophagoides farinae and Dermatophagoides pteronyssinus. J Agric Food Chem. 2007 Jul 25;55(15):6027-31. [Content Brief]

[4]. Yoou MS, et al. Ameliorative effect of atractylenolide III in the mast cell proliferation induced by TSLP. Food Chem Toxicol. 2017 Aug;106(Pt A):78-85. [Content Brief]

[5]. Zhou Y, et al. Atractylenolide III reduces depressive- and anxiogenic-like behaviors in rat depression models. Neurosci Lett. 2021 Aug 10;759:136050. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0271 mL	20.1353 mL	40.2706 mL	100.6765 mL
	5 mM	0.8054 mL	4.0271 mL	8.0541 mL	20.1353 mL
	10 mM	0.4027 mL	2.0135 mL	4.0271 mL	10.0677 mL
	15 mM	0.2685 mL	1.3424 mL	2.6847 mL	6.7118 mL
	20 mM	0.2014 mL	1.0068 mL	2.0135 mL	5.0338 mL
	25 mM	0.1611 mL	0.8054 mL	1.6108 mL	4.0271 mL
	30 mM	0.1342 mL	0.6712 mL	1.3424 mL	3.3559 mL
	40 mM	0.1007 mL	0.5034 mL	1.0068 mL	2.5169 mL
	50 mM	0.0805 mL	0.4027 mL	0.8054 mL	2.0135 mL
	60 mM	0.0671 mL	0.3356 mL	0.6712 mL	1.6779 mL
	80 mM	0.0503 mL	0.2517 mL	0.5034 mL	1.2585 mL
	100 mM	0.0403 mL	0.2014 mL	0.4027 mL	1.0068 mL

Atractylenolide III Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atractylenolide III73030-71-4ICodonolactone 8β-HydroxyasterolideApoptosisgastroprotectiveanxiogenicA549HUVECInhibitorinhibitorinhibit

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Atractylenolide III (Synonyms: ICodonolactone; 8β-Hydroxyasterolide)

Customer Review

Other Forms of Atractylenolide III:

Atractylenolide III Related Antibodies

3 Publications Citing Use of MCE Atractylenolide III

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Biological Activity

Purity & Documentation

References

Customer Review

Atractylenolide III Related Antibodies

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Complete Stock Solution Preparation Table

Atractylenolide III Related Classifications

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