Atractylenolide III
Based on 3 publication(s) in Google Scholar
Atractylenolide III (ICodonolactone) is the main component of Atractylodes rhizome and has the activity of inducing apoptosis in lung cancer cells. Atractylenolide III is an orally active gastroprotective agent.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 73030-71-4
- Formula: C15H20O3
- Molecular Weight:248.32
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Atractylenolide III
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WB
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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Histological Imaging/Staining
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
100.7 μM
Compound: 1
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Inhibition of human SOAT2-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis
Inhibition of human SOAT2-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis
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[PMID: 32035699] |
| CHO | IC50 |
174.9 μM
Compound: 1
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Inhibition of human SOAT1 mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis
Inhibition of human SOAT1 mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis
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[PMID: 32035699] |
| CHO | IC50 |
187.3 μM
Compound: 1
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Inhibition of human SOAT2-mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis
Inhibition of human SOAT2-mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis
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[PMID: 32035699] |
| CHO | IC50 |
55.1 μM
Compound: 1
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Inhibition of human SOAT1-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis
Inhibition of human SOAT1-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis
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[PMID: 32035699] |
| CHO-K1 | IC50 |
>195 μM
Compound: 1
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Inhibition of [14C]triglyceride biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method
Inhibition of [14C]triglyceride biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method
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[PMID: 32035699] |
| CHO-K1 | IC50 |
>195 μM
Compound: 1
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Cytotoxicity against CHOK1 cells assessed as reduction in cell viability
Cytotoxicity against CHOK1 cells assessed as reduction in cell viability
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[PMID: 32035699] |
| CHO-K1 | IC50 |
211 μM
Compound: 1
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Inhibition of SOAT in CHOK1 microsomes
Inhibition of SOAT in CHOK1 microsomes
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[PMID: 32035699] |
| CHO-K1 | IC50 |
73.5 μM
Compound: 1
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Inhibition of [14C]cholesterol ester biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method
Inhibition of [14C]cholesterol ester biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method
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[PMID: 32035699] |
| PC-12 | EC50 |
36.3 μM
Compound: 16; 19
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Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay
Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay
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[PMID: 29775304] |
| RAW264.7 | IC50 |
>100 μM
Compound: 8
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Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 24 hrs in presence of LPS by colorimetric analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 24 hrs in presence of LPS by colorimetric analysis
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[PMID: 27865705] |
Atractylenolide III (1-100 μM, 48 h ) induces cell apoptosis, and induces the activation of caspase-3 and caspase-9 and cleavage of PARP in A549 cells[1].
Atractylenolide III (1-100 μM, 72 h) inhibits proliferation and angiogenesis (tube formation) in HUVECs[1].
Atractylenolide III (1-100 μM) inhibits thymic stromal lymphopoietin (TSLP)-induced production of proinflammatory cytokines (IL-6, IL-1b, TNF-α, and IL-8) in HMC-1 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549
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Concentration:1, 10, 100 μM
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Incubation Time:24 h, 48 h
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Result:Increased active form of caspase-9, caspase-3, and the cleavage of PARP at 48 h. Increased the expression of proapoptotic protein bax, and induced AIF translocation to the nucleus at 24 h.
Atractylenolide III (30 mg/kg, oral gavage for 14 days or 28 days) reduces depressive- and anxiogenic-like behaviors in rat depression models in Lipopolysaccharide (LPS) (HY-D1056) and chronic unpredictable mild stress (CUMS) induced rat depression model[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:70% ethanol-induced gastric ulcers in rats[2]
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Dosage:5 and 10 mg/kg
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Administration:p.o.
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Result:Inhibited gastric ulcer formation and the necrotic erosion of gastric mucosa.
Downregulated the MMP-2/9 expression by activating the TIMP-2 and TIMP-1 expressions.
Chemical Information
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CAS No. 73030-71-4
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Appearance Solid
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Molecular Weight 248.32
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Formula C15H20O3
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Color Off-white to light yellow
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SMILES
O=C1C(C)=C(C[C@@]23[H])[C@@](C[C@@]3(C)CCCC2=C)(O)O1
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Synonyms
ICodonolactone; 8β-Hydroxyasterolide
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Inhibition of ASCT2 induces hepatic stellate cell senescence with modified proinflammatory secretome through an IL-1 α/NF- κ B feedback pathway to inhibit liver fibrosis. [Abstract]2022 Sep;12(9):3618-3638. PMID: 36176909
Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]
Protein expression of ASCT2 in atractylenolide III (0, 1, 2, 5, 10, 20, 40, 80, 100, 200 μM) concentration gradient-treated HSC-LX2 cells by Western blot.
Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]
Intracellular glutamine, glutamate, α-KG, GSH and ATP levels were evaluated by kits in atractylenolide III (40 μM)-treated HSC-LX2 cells.
Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]
Cell viability was tested in re-expression ASCT2-WT or ASCT2-N230G into atractylenolide III-treatment LX2 cells.
Atractylenolide III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2022 Sep;12(9):3618-3638. [Abstract]
Representative images of SA-β-Gal staining and α-SMA IHC in cryosections from mouse liver sections treated with Atractylenolide III (40 mg/kg).
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Pharmacol Res
Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]2020 May;155:104751. PMID: 32151678 -
J Cell Mol Med
Atractylodes macrocephala III suppresses EMT in cervical cancer by regulating IGF2BP3 through ETV5. [Abstract]2024 Feb;28(4):e18081. PMID: 38358034
Solvent & Solubility
DMSO : 50 mg/mL (201.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Kang TH, et al. Atractylenolide III, a sesquiterpenoid, induces apoptosis in human lung carcinoma A549 cells via mitochondria-mediated death pathway. Food Chem Toxicol. 2011 Feb;49(2):514-9. [Content Brief]
[2]. Wang KT, et al. Gastroprotective activity of atractylenolide III from Atractylodes ovata on ethanol-induced gastric ulcer in vitro and in vivo. J Pharm Pharmacol. 2010 Mar;62(3):381-8. [Content Brief]
[3]. Kim HK, et al. Toxicity of atractylon and atractylenolide III Identified in Atractylodes ovata rhizome to Dermatophagoides farinae and Dermatophagoides pteronyssinus. J Agric Food Chem. 2007 Jul 25;55(15):6027-31. [Content Brief]
[4]. Yoou MS, et al. Ameliorative effect of atractylenolide III in the mast cell proliferation induced by TSLP. Food Chem Toxicol. 2017 Aug;106(Pt A):78-85. [Content Brief]
[5]. Zhou Y, et al. Atractylenolide III reduces depressive- and anxiogenic-like behaviors in rat depression models. Neurosci Lett. 2021 Aug 10;759:136050. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0271 mL | 20.1353 mL | 40.2706 mL | 100.6765 mL |
| 5 mM | 0.8054 mL | 4.0271 mL | 8.0541 mL | 20.1353 mL | |
| 10 mM | 0.4027 mL | 2.0135 mL | 4.0271 mL | 10.0677 mL | |
| 15 mM | 0.2685 mL | 1.3424 mL | 2.6847 mL | 6.7118 mL | |
| 20 mM | 0.2014 mL | 1.0068 mL | 2.0135 mL | 5.0338 mL | |
| 25 mM | 0.1611 mL | 0.8054 mL | 1.6108 mL | 4.0271 mL | |
| 30 mM | 0.1342 mL | 0.6712 mL | 1.3424 mL | 3.3559 mL | |
| 40 mM | 0.1007 mL | 0.5034 mL | 1.0068 mL | 2.5169 mL | |
| 50 mM | 0.0805 mL | 0.4027 mL | 0.8054 mL | 2.0135 mL | |
| 60 mM | 0.0671 mL | 0.3356 mL | 0.6712 mL | 1.6779 mL | |
| 80 mM | 0.0503 mL | 0.2517 mL | 0.5034 mL | 1.2585 mL | |
| 100 mM | 0.0403 mL | 0.2014 mL | 0.4027 mL | 1.0068 mL |
Atractylenolide III Related Classifications
HY-N0203 Related Classifications
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