Asiatic acid 

Asiatic acid, a pentacyclic triterpene found in Centella asiatica (Centella asiatica), has anticancer activity. Asiatic acid induces apoptosis in melanoma cells and has barrier protective effects on human aortic endothelial cells (HAEC). Asiatic acid also has anti-inflammatory activity and inhibits tumor necrosis factor (TNF)-α-induced endothelial barrier dysfunction. Asiatic acid also inhibits NLRP3 inflammasome activation and NF-κB pathway, effectively inhibits inflammation in rats, and has neuroprotective effects in rat spinal cord injury (SCI) model^{[1][2][3][4][5]}.

Asiatic acid exerts barrier stabilizing effects against TNF-α by maintaining adherens junctions (AJs) and inhibiting tight junction (TJ) redistribution^[2].
Asiatic acid (40 μM) stabilizes F-actin and diphosphate-MLC at the cell periphery and prevents their rearrangement stimulated by TNF-α (10 ng/mL; 1 h). However, Asiatic acid (10-30 μM; 6 h) cannot reduce F-actin aggregation^[2].
Asiatic acid (40 μM; 6 h) preserves the network structure of cell-cell contact areas and abolishes TNF-α-induced structural reorganization of vascular endothelial (VE)-cadherin and β-catenin^[2].
However, Asiatic acid (10-40 μM; 6 h) had no inhibitory effect on endothelial cell permeability increased by cytochalasin D (5 μM; 15 min). Asiatic acid increases the diphosphorylation of myosin light chain (MLC)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Asiatic acid (5-75 mg/kg; intragastric gavage once daily) effectively inhibited pelvic inflammatory disease (PID) in rats in a pathogen-induced disease model and exerted antioxidant and anti-inflammatory effect^[3].
Asiatic acid (30 mg/kg, 75 mg/kg; intragastric injection, once daily) has neuroprotective effects on spinal cord injury (SCI) in rats by inhibiting inflammation and oxidative stres^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

488.70

C₃₀H₄₈O₅

464-92-6

Solid

White to off-white

C[C@@]1(CO)[C@@H](O)[C@H](O)C[C@]2(C)[C@@]3([H])CC=C4[C@]5([H])[C@@H](C)[C@H](C)CC[C@@](C(O)=O)5CC[C@](C)4[C@@](C)3CC[C@@]12[H]

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Park BC, et al. Asiatic acid induces apoptosis in SK-MEL-2 human melanoma cells. Cancer Lett. 2005 Jan 31;218(1):81-90.  [Content Brief]

  [2]. Huang SS, et al. Antinociceptive activities and the mechanisms of anti-inflammation of asiatic Acid in mice. Evid Based Complement Alternat Med. 2011;2011:895857.  [Content Brief]

  [3]. Fong LY, et al. Asiatic acid stabilizes cytoskeletal proteins and prevents TNF-α-induced disorganization of cell-cell junctions in human aortic endothelial cells. Vascul Pharmacol. 2019 Jun;117:15-26.  [Content Brief]

  [4]. Jiang W, et al. Neuroprotective effect of asiatic acid against spinal cord injury in rats. Life Sci. 2016 Jul 15;157:45-51.  [Content Brief]

  [5]. Kong D, et al. Protective effects of Asiatic acid against pelvic inflammatory disease in rats. Exp Ther Med. 2019 Jun;17(6):4687-4692.  [Content Brief]